Theoretical and Applied Genetics

, Volume 95, Issue 4, pp 553–567 | Cite as

Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.)

  • X. Chen
  • S. Temnykh
  • Y. Xu
  • Y. G. Cho
  • S. R. McCouch

Abstract

 Ninety-four newly developed microsatellite markers were integrated into existing RFLP framework maps of four rice populations, including two doubled haploid, a recombinant inbred, and an interspecific backcross population. These simple sequence repeats (SSR) were predominantly poly(GA) motifs, targetted because of their abundance in rice. They were isolated from a previously described sheared library and a newly constructed enzyme-digested library. Differences in the average length of poly(GA) tracts were observed for clones isolated from the two libraries. The length of GA motifs averaged 21 repeat units for clones isolated from the Tsp-509-digested library, while motifs averaged 17 units for clones from the sheared library. There was no evidence of clustering of microsatellite markers near centromeres or telomeres. Mapping of the 94 newly developed markers as well as of 27 previously reported microsatellites provided genome-wide coverage of the 12 chromosomes, with an average distance of 1 SSLP (simple sequence repeat polymorphism) per 16–20 cM.

Key words Microsatellite Simple sequence repeats (SSR) Simple sequence length polymorphism(SSLP) Linkage map Molecular map Rice (Oryza sativa) 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • X. Chen
    • 1
  • S. Temnykh
    • 1
  • Y. Xu
    • 1
  • Y. G. Cho
    • 2
  • S. R. McCouch
    • 1
  1. 1.Department of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USAUS
  2. 2.National Institute of Agricultural Science and Technology, Suweon, KoreaKR

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