Advertisement

Theoretical and Applied Genetics

, Volume 95, Issue 1–2, pp 59–65 | Cite as

The α-gliadin gene family. II. DNA and protein sequence variation, subfamily structure, and origins of pseudogenes

II. DNA and protein sequence variation, subfamily structure, and origins of pseudogenes
  • O. D. Anderson
  • F. C. Greene

Abstract

 The derived amino-acid sequences of all reported α-gliadin clones are compared and analyzed, and the patterns of sequence change within the α-gliadin family are examined. The most variable sequences are two polyglutamine domains. These two domains are characteristic features of the α-gliadin storage proteins and account for most of the variation in protein size of this otherwise highly conserved protein family. In addition, their encoding DNA sequences form microsatellites. Single-base substitutions in the α-gliadin genes show a preponderance of transitions, including the C to T substitution which contributes to the generation of stop codons, and consequently to the observation that approximately 50% of the α-gliadin genes are pseudogenes. In one unusual gene, a microsatellite has expanded to 321 bp as compared to the normal 36–72 bp, and may result from similar mechanisms that produce polyglutamine-associated genetic diseases in humans. A comparison of the 27 reported sequences show several α-gliadin gene subfamilies, at least some of which are genome specific.

Key words α-Gliadin Microsatellites Sequence variation Pseudogenes Polyglutamine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • O. D. Anderson
    • 1
  • F. C. Greene
    • 1
  1. 1.USDA, ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USAUS

Personalised recommendations