Theoretical and Applied Genetics

, Volume 94, Issue 6–7, pp 947–955 | Cite as

Phylogenetic relationships of coffee-tree species (Coffea L.) as inferred from ITS sequences of nuclear ribosomal DNA

  • P. Lashermes
  • M. C. Combes
  • P. Trouslot
  • A. Charrier

Abstract

 Phylogenetic relationships of Coffea species were estimated from the sequences of the internal transcribed spacer (ITS 2) region of nuclear ribosomal DNA. The ITS 2 region of 37 accessions belonging to 26 Coffea taxa and to three Psilanthus species was directly sequenced from polymerase chain reaction (PCR)-amplified DNA fragments. The level of variation was high enough to make the ITS 2 a useful tool for phylogenetic reconstruction. However, an unusual level of intraspecific variation was observed leading to some difficulty in interpreting rDNA sequence divergences. Sequences were analysed using Wagner parsimony as well as the neighbour-joining distance method. Coffea taxa were divided into several major groups which present a strong geographical correspondence (i.e. Madagascar, East Africa, Central Africa and West Africa). This organisation is well supported by cytogenetic evidence. On the other hand, the results were in contradiction with the present classification of coffee-tree taxa into two genera, namely Coffea and Psilanthus. Furthermore, additivity of parental rDNA types was not observed in the allotetraploid species C. arabica.

Key words Coffea Coffee-tree Internal transcribed spacer region Nuclear ribosomal DNA Molecular phylogeny 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • P. Lashermes
    • 1
  • M. C. Combes
    • 1
  • P. Trouslot
    • 1
  • A. Charrier
    • 2
  1. 1.ORSTOM, Laboratoire de ressources génétiques et d’amélioration des plantes tropicales, BP 5045, F-34032, Montpellier, FranceFR
  2. 2.ENSAM, place Viala, F-34060, Montpellier, FranceFR

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