Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Production and cytogenetics of Brassica campestris-alboglabra chromosome addition lines

Abstract

 Four different Brassica campestris-alboglabra monosomic addition lines (AA+1 chromosome from C, 2n=21) were obtained after consecutive backcrosses between resynthesized B. napus (AACC, 2n=38) and the parental B. campestris (AA, 2n=20) accession. The alien chromosomes of B. alboglabra (CC, 2n=18) in the addition lines were distinguished by random amplified polymorphic DNA (RAPD) marker analysis and morphology of mitotic chromosomes. Four RAPD marker synteny groups were established, which represented the four different alien chromosomes of B. alboglabra in the four addition lines. Three of the four addition lines were identified to harbour chromosomes 4, 8 or 9 of B. alboglabra. Studies on meiotic pairing in the addition lines revealed intergenomic homoeology relationships among specific chromosome arms between the A- and C-genomes. The long arm of B. campestris chromosome 9 was homoeologous with the long arm of B. alboglabra chromosome 4, while its short arm with the short arms of B. alboglabra chromosomes 8 and 9. Such an intergenomic homoeology relationship supports the hypothesis that B. campestris and B. alboglabra share a common ancestor but that chromosomal rearrangements have occurred during the evolution of the two species. Intergenomic introgression was observed in the progenies of the addition lines. The introgression of an entire B. alboglabra marker synteny group into the B. campestris genome implied the possible occurrence of interspecific chromosomal substitution.

This is a preview of subscription content, log in to check access.

Author information

Additional information

Received: 30 May 1996 / Accepted: 18 October 1996

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chen, B., Cheng, B., Jørgensen, R. et al. Production and cytogenetics of Brassica campestris-alboglabra chromosome addition lines. Theor Appl Genet 94, 633–640 (1997). https://doi.org/10.1007/s001220050460

Download citation

  • Key words Brassica species
  • Addition line
  • RAPD marker
  • Meiotic pairing
  • Intergenomic homoeology