Theoretical and Applied Genetics

, Volume 94, Issue 5, pp 564–568 | Cite as

Linkage mapping of starch branching enzyme III in rice (Oryza sativa L.) and prediction of location of orthologous genes in other grasses

  • S. E. Harrington
  • H. F. J. Bligh
  • W. D. Park
  • C. A. Jones
  • S. R. McCouch

Abstract

 The chromosomal position of Starch Branching Enzyme III (SBEIII) was determined via linkage to RFLP markers on an existing molecular map of rice (Oryza sativa L.). A cDNA of 890 bp was generated using specific PCR primers designed from available SBEIII sequence data and used as a probe in Southern analysis. The SBEIII cDNA hybridized to multiple restriction fragments, but these fragments mapped to a single locus on rice chromosome 2, flanked by CDO718 and RG157. The detection of a multiple-copy hybridization pattern suggested the possibility of a tandemly duplicated gene at this locus. The map location of orthologous SBE genes in maize, wheat, and oat were predicted based on previously published genetic studies and comparative maps of the grass family.

Key words Rice Molecular mapping Grain quality Starch branching enzyme (SBE) Amylose extender (ae) 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • S. E. Harrington
    • 1
  • H. F. J. Bligh
    • 2
  • W. D. Park
    • 3
  • C. A. Jones
    • 2
  • S. R. McCouch
    • 1
  1. 1.Department of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1902, USAUS
  2. 2.Department of Biochemistry, Floor D, Medical School, Queen’s Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UKGB
  3. 3.Department of Biochemistry, Texas A&M University, College Station, TX 77843XX

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