Theoretical and Applied Genetics

, Volume 94, Issue 2, pp 249–254 | Cite as

Microsatellites in Malus X domestica (apple): abundance, polymorphism and cultivar identification

  • P. Guilford
  • S. Prakash
  • J. M. Zhu
  • E. Rikkerink
  • S. Gardiner
  • H. Bassett
  • R. Forster

Abstract

Screening of an apple genomic library with (GA)15 and (GT)15 probes demonstrated that these repeats are abundant, occurring about every 120 and 190 kb, respectively. Microsatellites isolated from a small insert library enriched for (GA) repeats contained numbers of repeats ranging from 7 to 39. Primers to these microsatellite loci were able to direct the amplification of the repeats in 21 different cultivars. The majority of markers were highly polymorphic, diploid, and showed simple Mendelian inheritance, although about 25% of markers generated complex banding patterns consistent with the amplification of more than one locus. As few as three microsatellite markers were sufficient to differentiate between all 21 cultivars.

Key words Microsatellites Apple Identification 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • P. Guilford
    • 1
  • S. Prakash
    • 1
  • J. M. Zhu
    • 1
  • E. Rikkerink
    • 1
  • S. Gardiner
    • 2
  • H. Bassett
    • 2
  • R. Forster
    • 1
  1. 1.Horticulture and Food Research Institute of New Zealand, Mt Albert Research Centre, Private Bag 92 169, Auckland, New ZealandNZ
  2. 2.Bachelar Research Centre, Private Bag 11 030, Palmerston North, New ZealandNZ

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