Theoretical and Applied Genetics

, Volume 94, Issue 1, pp 34–38 | Cite as

Identifying different types of de-differentiated microspores from indica-japonica F1 hybrids with subspecies-differentiating RFLP probes in rice

  • J. -H. Xie
  • M. -W. Gao
  • J. Lu
  • J. -Y. Zhuang
  • H. -X. Lin
  • H. -R. Qian
  • K. -L. Zheng
Article
Received:
Accepted:

Abstract

The indica, japonica and intermediary types of de-differentiated microspores from indica-japonica F1 hybrids were identified with 11 subspecies-differentiating RELP probes in rice (Oryza sativa L.). The results showed that the distribution of indica, japonica and intermediary types of de-differentiated microspores could be easily detected in a simple and quick way using the RFLP method. Moreover, the microspores from the same F1 hybrid but inoculated onto different media, or microspores from different F1 hybrids when inoculated onto the same medium, often displayed distinctive distribution curves of de-differentiated microspores types, indicating that the media employed in this experiment had high selectivity for the de-differentiation of certain types of microspores. The application of the RELP method to de-differentiated microspore identification is of great theoretical and practical significance in rice doubled-haploid breeding.

Key words

De-differentiated microspore Indica-japonica F1 hybrid Restriction fragment length polymorphism (RFLP) Subspecies-differentiating probe Oryza sativa L. 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arnison PG, Donaldson P, Jackson A, Semple C, Keller W (1990) Genotype-specific responce of cultured broccoli (Brassica oleracea var. italica) anthers to cytokinins. Plant Cell Tissue Org Cult 20:217–222Google Scholar
  2. Cai Y, Hu Z, Huang S (1983) Applications to rice anther intersub-species improvement. In: Shen J, Zhang Z, Shi S (eds) Rice anther culture breeding. Agricultural Science Press, Beijing pp 201–202Google Scholar
  3. Chen Y, Tian W, Zheng S, Li L (1991) Influence of some factors on induction frequency and effect of genotype in anther culture of Oryza sativa subsp. indica. Acta Gene Sinica 18:358–365Google Scholar
  4. Cheng KS (1985) A statistical evaluation of the classification of rice cultivars into hsien and keng subspecies. R G N 2:46–48Google Scholar
  5. Cho MS, Zapata FJ (1990) Plant regeneration from isolated microspores of indica rice. Plant Cell Physiol 31:881–885Google Scholar
  6. Glaszmann JC (1987) Isozymes and classification of Asian varieties. Theor Appl Genet 74:21–30CrossRefGoogle Scholar
  7. Li MF (1991) Rice anther culture for breeding, In: Yan C J (ed) Tissue culture of field crops. Shanghai Press, Shanghai, pp 135–152Google Scholar
  8. Lu Y, Zheng KL (1992) A simple method for the extraction of rice DNA. Chinese Rice Sci 6:47–48Google Scholar
  9. McCouch SR, Tanksley SD (1991) Development and use of restriction fragment length polymorphism in rice breeding and generics. In: Khush GS, Toenniessen GH (eds) Rice biotechnology. C.A.B. International, UK, pp 109–133Google Scholar
  10. McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829CrossRefGoogle Scholar
  11. Morishima H, Oka HI (1981) Phylogenetic differentiation of cultivated rice. XXII. Numeral evaluation of the indica-japonica differentiation. Japan J Breed 31:402–414Google Scholar
  12. Oba S, Hanzawa M, Uchimiya H (1991) Estimates of chromosome recombination by RFLPs and search for indica-specific DNA probes. In: Proc 3rd Int Workshop Rice Mol Biol, Hokkaido University, Sapporo, Japan, pp 42Google Scholar
  13. Oka HI (1992) Genetic diversity of wild and cultivated rice, In: Khush GS, Toenniessen GH (eds) Rice biotechnology. C.A.B. International, UK, pp 55–81Google Scholar
  14. Pescitelli SM, Mitchell JC, Jones AM, Pareddy DR, Petolino JF (1989) High frequency androgenesis from isolated microspores of maize. Plant Cell Rep 7:673–676Google Scholar
  15. Qian H R, Zhuang J Y, Lin H X, Lu J, Zheng K L (1995) Identification of a set of RFLP probes for subspecies differentiation in Oryza sativa L. Theor Appl Genet 90:878–884CrossRefGoogle Scholar
  16. Saghai Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mandelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018CrossRefGoogle Scholar
  17. Shen ZT, Xue Q, Liu J, Guo Q, Wang Z, Liang Z (1978) Genetic performance of rice pollen plants, In: Anther culture. Science press, Beijing, pp 255–257Google Scholar
  18. Tanksley SD, Young ND, Paterson AH, Bonierabale MW (1989) RFLP mapping in plant breeding — new tools for an old science. Biotechnology 7:257–264CrossRefGoogle Scholar
  19. Xie JH, Gao MW, Cai QH, Cheng XY, Shen YW, Liang ZQ (1995) Improved isolated microspore culture effeciency in medium with maltose and optimized growth regulator combination in japonica rice (Oryza sativa), Plant Cell Tissue Org Cult 42:245–250CrossRefGoogle Scholar
  20. Xue QZ (1991) Genetic variation of pollen plants in rice. In: Yan C J (ed) Tissue culture of field crops. Shanghai Press, Shanghai, pp 170–176Google Scholar
  21. Zheng KL, Shen B, Yu F, Zhao CZ, Qi XF, Xu XM (1990) Restriction fragment length polymorphisms in rice. Chinese J Rice Sci 4:145–149Google Scholar
  22. Zheng KL, Qian HR, Shen B, Zhaung JY, Lin HX, Lu J (1994) RFLP-based phylogenetic analysis of wide compatibility varieties in Oryza sativa L. Theor Appl Genet 88:65–69CrossRefGoogle Scholar
  23. Zhu DY, Ding XH, Yi JH (1991) Anther culture and breeding in indica rice. In: Yan C J (ed) Tissue culture of field crops. Shanghai Press, Shanghai, pp 153–169Google Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • J. -H. Xie
    • 1
  • M. -W. Gao
    • 1
  • J. Lu
    • 2
  • J. -Y. Zhuang
    • 2
  • H. -X. Lin
    • 2
  • H. -R. Qian
    • 2
  • K. -L. Zheng
    • 2
  1. 1.Institute of Nuclear Agriculture SciencesZhejiang Agricultural UniversityHangzhouChina
  2. 2.Biotechnology DepartmentChina National Rice Research InstituteHangzhouChina

Personalised recommendations