Theoretical and Applied Genetics

, Volume 100, Issue 3–4, pp 498–505 | Cite as

Microsatellite variability in grapevine cultivars from different European regions and evaluation of assignment testing to assess the geographic origin of cultivars

  • K. M. Sefc
  • M. S. Lopes
  • F. Lefort
  • R. Botta
  • K. A. Roubelakis-Angelakis
  • J. Ibáñez
  • I. Pejić
  • H. W. Wagner
  • J. Glössl
  • H. Steinkellner
Original Article

Abstract 

Nine microsatellite markers (VVMD5, VVMD7, VVS2, ssrVrZAG21, ssrVrZAG47, ssrVrZAG62, ssrVrZAG64, ssrVrZAG79 and ssrVrZAG83) were chosen for the analysis of marker information content, the genetic structure of grapevine cultivar gene pools, and differentiation among grapevines sampled from seven European vine-growing regions (Greece, Croatia, North Italy, Austria and Germany, France, Spain and Portugal). The markers were found to be highly informative in all cultivar groups and therefore constitute a useful set for the genetic characterization of European grapevines. Similar and high levels of genetic variability were detected in all investigated grapevine gene pools. Genetic differentiation among cultivars from different regions was significant, even in the case of adjacent groups such as the Spanish and Portuguese cultivars. No genetic differentiation could be detected between vines with blue and white grapes, indicating that they have undergone the processes of cultivar development jointly. The observed genetic differentiation among vine-growing regions suggested that cultivars could possibly be assigned to their regions of origin according to their genotypes. This might allow one to determine the geographical origin of cultivars with an unknown background. The assignment procedure proved to work for cultivars from the higher differentiated regions, as for example from Austria and Portugal.

Key words Vitis Microsatellites Genetic variation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • K. M. Sefc
    • 1
  • M. S. Lopes
    • 2
  • F. Lefort
    • 3
  • R. Botta
    • 4
  • K. A. Roubelakis-Angelakis
    • 3
  • J. Ibáñez
    • 5
  • I. Pejić
    • 6
  • H. W. Wagner
    • 7
  • J. Glössl
    • 1
  • H. Steinkellner
    • 1
  1. 1.Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: steink@mail.boku.ac.atAT
  2. 2.Universidade dos Açores, Departamento de Ciências Agrárias, Terra Chã, 9700 Angra do Heroísmo, Açores, PortugalPT
  3. 3.Laboratory of Plant Physiology and Biotechnology, Department of Biology, University of Crete, Vassilika Vouton, P.O. Box 2208, 71409 Heraklion, CreteXX
  4. 4.Università degli Studi di Torino, Dipartimento di Colture Arboree, Via Leonardo da Vinci 44, 10095 Grugliasco (TO), ItalyIT
  5. 5.Finca ”El Encín” Km 38,200 Crta. N-II, Abdo. 127 Alcalá de Henares, 28800 Madrid, SpainES
  6. 6.Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, HR-10000 Zagreb, Svetosimunska 25, CroatiaXX
  7. 7.Institut für Allgemeine Physik, Technische Universität Wien, Wiedner Hauptstraße 8–10, A-1040 Vienna, AustriaAT

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