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Theoretical and Applied Genetics

, Volume 100, Issue 3–4, pp 461–470 | Cite as

Evidence of multiple complex patterns of T-DNA integration into the rice genome

  • Z. Yin
  • G.-L. Wang
Original Article

Abstract 

The transfer of the long T-DNA (T-DNA and non-T-DNA) of a binary plasmid from Agrobacterium into the rice genome was investigated at both molecular and genetic levels. Out of 226 independent transgenic plants, 33% of the transformants contained non-T-DNA sequences. There was no major difference in the frequency of non-T-DNA transfer among three Agrobacterium tumefaciens strains.Four T1 plants containing a single putative long T-DNA insertion were selected for Southern analysis. Three of them were confirmed to have a long T-DNA insertion with a size of greater-than-unit-length of the binary plasmid. This was further confirmed by rescuing the intact binary plasmid from these plants. Our results suggest that long T-DNA transfer by rolling-circle replication from Agrobacterium to rice occurs frequently, and that the high frequency of non-T-DNA transfer should be considered when producing transgenic rice for commercial production.

Key words Agrobacterium tumefaciens T-DNA Non-T-DNA Long T-DNA transfer Rice transformation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Z. Yin
    • 1
  • G.-L. Wang
    • 1
  1. 1.Institute of Molecular Agrobiology, 1 Research Link, The National University of Singapore, Singapore 117604, Republic of Singapore e-mail: wanggl@ima.org.sg Fax: 65-872 7007SG

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