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Theoretical and Applied Genetics

, Volume 100, Issue 1, pp 49–56 | Cite as

A combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for root-penetration ability

  • A. H. Price
  • K. A. Steele
  • B. J. Moore
  • P. P. Barraclough
  • L. J. Clark
Original paper

Abstract 

Acombined RFLP and AFLP linkage map of an F6 recombinant inbred population, which was derived from a previously mapped F2 of a cross between the two drought resistant upland rice varieties Bala and Azucena, is presented. The map contains 101 RFLP and 34 AFLP markers on 17 linkage groups covering 1680 cM. Also presented is the approximate mapping position of a further four RFLP and 75 AFLP markers, which either could not be given a unique place on the map or for which the available data is not sufficient to allow confident positioning, and the result of quantitative trait locus (QTL) mapping of traits related to root-penetration ability. Root penetration was assessed by counting the number of root axes that penetrated a 3 mm-thick layer consisting of 80% wax and 20% white soft paraffin. Good root penetration would be expected to increase drought resistance where soil strength is high. Single-marker analysis revealed seven QTLs for the number of roots which penetrate the wax layer. In identical locations were seven QTLs for the ratio of penetrated to the total number of roots. Transgressive inheritance of positive alleles from Bala explained four of these QTLs. Comparison of the QTLs identified here with previous reports of QTLs for root morphology suggest that alleles which improve root penetration ability may also either make the roots longer or thicker.

Key words Drought resistance Oryza sativa QTL Rice Root penetration 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • A. H. Price
    • 1
  • K. A. Steele
    • 2
  • B. J. Moore
    • 2
  • P. P. Barraclough
    • 3
  • L. J. Clark
    • 3
  1. 1.Department of Plant and Soil Science, University of Aberdeen, Aberdeen AB24 3UU, UK e-mail: a.price@abdn.ac.uk, Fax: +44 (0)1224 272703GB
  2. 2.Centre for Arid Zone Studies, University of Wales, Bangor, Gwynedd LL57 2UW, UKGB
  3. 3.IACR-Rothamsted, Harpenden, Hertfordshire, AL5 2JQ, UKGB

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