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Theoretical and Applied Genetics

, Volume 100, Issue 1, pp 17–26 | Cite as

Introgression of Allium fistulosum into A. cepa mediated by A. roylei

  • L. I. Khrustaleva
  • C. Kik
Original Article

Abstract 

Introgression of Allium fistulosum into the genome of A. cepa using A. roylei as a bridging species was studied by means of genomic in situ hybridization (GISH). Here we demonstrate for the first time that A. fistulosum can be stably introgressed into A. cepa with a bridge-cross. The first and second bridge-cross generations were fertile, although pollen was sterile in some individuals. Only occasionally were there translocations in the second generation bridge-cross. Recombination between the three genomes was frequently seen in meiotic anaphase 1 and prophase 2 chromosomes of the first generation bridge cross and in mitotic chromosomes of the second generation bridge-cross. The number of observed recombination points in anaphase 1 and prophase 2 significantly exceeded the value expected from chiasma frequency in metaphase 1. Recombination points were randomly distributed, thus the A. cepa or A. roylei type of random distribution prevails over the A. fistulosum type of proximally localised chiasmata.

Key words Onion Wild species Multi-colour genomic in situ hybridisation Chiasmata Meiotic recombination 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • L. I. Khrustaleva
    • 1
  • C. Kik
    • 1
  1. 1.DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Vegetable and Fruit Crops, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: c.kik@cpro.dlo.nl, Fax: 31 317 418094NL

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