Theoretical and Applied Genetics

, Volume 102, Issue 6–7, pp 885–891 | Cite as

Detection of in vitro culture-induced instability through inter-simple sequence repeat analysis

  • X. J. Leroy
  • K. Leon
  • J.M. Hily
  • P. Chaumeil
  • M. Branchard
Original Article

Abstract 

This paper reports on investigations focused on trinucleotide and tetranucleotide repeats in cauliflower calli (Brassica oleracea var. botrytis L.) and carried out to determine their utility in the detection of genetic variations induced by tissue culture. Out of 224 calli 6 exhibited original patterns; in one of these, PCR patterns differed at four polymorphic loci. The observed tetranucleotide-repeat classes were polymorphic, whereas fingerprinting patterns were stable with (CAG)5. The most frequent polymorphic and useful primer for detecting genetic variation appeared to be (CAA)5. We also characterised an Inter-Simple Sequence Repeat (ISSR) marker homologous to a gene involved in cellular proliferation, and modifications of this gene on callogenesis and/or differentiation are examined.

Keywords Calli Cauliflower Genetic instability Microsatellite primers Somaclonal variation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • X. J. Leroy
    • 1
  • K. Leon
    • 1
  • J.M. Hily
    • 1
  • P. Chaumeil
    • 1
  • M. Branchard
    • 1
  1. 1.Biotechnology and Plant Physiology Laboratory, Isamor – Brittany University, Technopôle Brest-Iroise, 29280 Plouzane, France, Present address: K. Leon, RhoBio Génomique, Genopole Evry, 2, Rue G. Crémieux, CP 5707, 91057 Evry Cedex, France, Present address: X.J. Leroy, Syngenta Crop Protection, WRO 1060-4.08, 4002 Basel, Switzerland e-mail: xavier.leroy@syngenta.com Fax: +41 61 3235 423FR

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