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A major yellow-seed QTL on chromosome A09 significantly increases the oil content and reduces the fiber content of seed in Brassica napus

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Abstract

Key message

A major yellow-seed QTL on chromosome A09 significantly increases the oil content and reduces the fiber content of seed in Brassica napus.

Abstract

The yellow-seed trait (YST) has always been a main breeding objective for rapeseed because yellow-seeded B. napus generally contains higher oil contents, fewer pigments and polyphenols and lower fiber content than black-seeded B. napus, although the mechanism controlling this correlation remains unclear. In this study, QTL mapping was implemented for YST based on a KN double haploid population derived from the hybridization of yellow-seeded B. napus N53-2 with a high oil content and black-seeded Ken-C8 with a relatively low oil content. Ten QTLs were identified, including four stable QTLs that could be detected in multiple environments. A major QTL, cqSC-A09, on chromosome A09 was identified by both QTL mapping and BSR-Seq technology, and explained more than 41% of the phenotypic variance. The major QTL cqSC-A09 for YST not only controls the seed color but also affects the oil and fiber contents in seeds. More importantly, the advantageous allele could increase the oil content and reduce the pigment and fiber content at the same time. This is the first QTL reported to control seed color, oil content and fiber content simultaneously with a large effect and has great application value for breeding high oil varieties with high seed quality. Important candidate genes, including BnaA09. JAZ1, BnaA09. GH3.3 and BnaA09. LOX3, were identified for cqSC-A09 by combining sequence variation annotation, expression differences and an interaction network, which lays a foundation for further cloning and breeding applications in the future.

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The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was financially supported by the National Natural Science Foundation of China (31871656, 32001583 and 32072098), and the Key Research Plan Project of Shaanxi Province (2020ZDLNY04-01).

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Authors and Affiliations

  1. School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China

    Hongbo Chao

  2. Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    Hongbo Chao, Liangxing Guo, Weiguo Zhao, Huaixin Li & Maoteng Li

  3. Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement, Yangling, 712100, China

    Weiguo Zhao

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  1. Hongbo Chao
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  2. Liangxing Guo
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  3. Weiguo Zhao
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  4. Huaixin Li
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  5. Maoteng Li
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Contributions

HC carried out QTL mapping and BSR analysis and wrote the manuscript. LG, WZ and HL participated in the field experiment and surveyed and analyzed the phenotypic data. ML designed the overall study and provided guidelines for writing the paper.

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Correspondence to Maoteng Li.

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Communicated by Jacqueline Batley.

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Chao, H., Guo, L., Zhao, W. et al. A major yellow-seed QTL on chromosome A09 significantly increases the oil content and reduces the fiber content of seed in Brassica napus. Theor Appl Genet 135, 1293–1305 (2022). https://doi.org/10.1007/s00122-022-04031-0

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