Eight environmentally stable QTL for grain yield-related traits were detected by four RIL populations, and two of them were validated by a natural wheat population containing 580 diverse varieties or lines.
Yield and yield-related traits are important factors in wheat breeding. In this study, four RIL populations derived from the cross of one common parent Yanzhan 1 (a Chinese domesticated cultivar) and four donor parents including Hussar (a British domesticated cultivar) and three semi-wild wheat varieties in China were phenotyped for 11 yield-related traits in eight environments. An integrated genetic map containing 2009 single-nucleotide polymorphism (SNP) markers generated from a 90 K SNP array was constructed to conduct quantitative trait loci (QTL) analysis. A total of 161 QTL were identified, including ten QTL for grain yield per plant (GYP) and yield components, 49 QTL for spike-related traits, 43 QTL for flag leaf-related traits, 22 QTL for plant height (PH), and 37 QTL for heading date and flowering date. Eight environmentally stable QTL were validated in individual RIL population where the target QTL was notably detected, and six of them had a significant effect on GYP. Furthermore, Two QTL, QSPS-2A.4 and QSL-4A.1, were also validated in a natural wheat population containing 580 diverse varieties or lines, which provided valuable resources for further fine mapping and genetic improvement in yield in wheat.
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Recombinant inbred line
Quantitative trait locus/loci
Best linear unbiased prediction
Joint inclusive composite interval mapping
Analysis of variance
Phenotypic variation explained
Grain yield per plant
Spike number per plant
Kernel number per spike
Spikelet number per spike
Flag leaf length
Flag leaf width
Flag leaf area
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This work was supported by the Transgenic Special Item of China (2016ZX08002003-002 and 2016ZX08009-003), the National Natural Science Foundation of China (31520103911, 31871610, 31901491 and 31901492), and the Agricultural Variety Improvement Project of Shandong Province (2019LZGC010).
Conflict of interests
The authors declare that they have no competing interests.
I declare on behalf of my co-authors that the work described is original, previously unpublished research, and not under consideration for publication elsewhere. The experiments in this study comply with the current laws of China.
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Communicated by Gary Muehlbauer.
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Hu, J., Wang, X., Zhang, G. et al. QTL mapping for yield-related traits in wheat based on four RIL populations. Theor Appl Genet 133, 917–933 (2020). https://doi.org/10.1007/s00122-019-03515-w