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Identification of a polymorphism within the Rosa multiflora muRdr1A gene linked to resistance to multiple races of Diplocarpon rosae W. in tetraploid garden roses (Rosa × hybrida)

Abstract

Key message

A QTL for resistance to several races of black spot co-located with the known Rrd1 locus in Rosa. A polymorphism in muRdr1A linked to black spot resistance was identified and molecular markers were designed.

Abstract

Black spot, caused by Diplocarpon rosae, is one of the most serious foliar diseases of landscape roses that reduces the marketability and weakens the plants against winter survival. Genetic resistance to black spot (BS) exists and race-specific resistance is a good target to implement marker-assisted selection. High-density single nucleotide polymorphism-based genetic maps were created for the female parent of a tetraploid cross between ‘CA60’ and ‘Singing in the Rain’ using genotyping-by-sequencing following a two-way pseudo-testcross strategy. The female linkage map was generated based on 227 individuals and included 31 linkage groups, 1055 markers, with a length of 1980 cM. Race-specific resistance to four D. rosae races (5, 7, 10, 14) was evaluated using a detached leaf assay. BS resistance was also evaluated under natural infection in the field. Resistance to races 5, 10 and 14 of D. rosae and field resistance co-located on chromosome 1. A unique sequence of 32 bp in exon 4 of the muRdr1A gene was identified in ‘CA60’ that co-segregates with D. rosae resistance. Two diagnostic markers, a presence/absence marker and an INDEL marker, specific to this sequence were designed and validated in the mapping population and a backcross population derived from ‘CA60.’ Resistance to D. rosae race 7 mapped to a different location on chromosome 1.

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Acknowledgments

This work was supported by funding from the Vineland Research and Innovation Centre, Agriculture and Agri-Food Canada Growing Forward 2 project #AIP-P013, the Canadian Nursery Landscape Association, Landscape Manitoba and Landscape Alberta.

Author information

CR and DS designed the experiments. CR implemented the experiments, conducted the phenotyping, mapping, and data analysis. TB and JO contributed to GBS library preparation and bioinformatics support. RL contributed to marker development. CR authored the manuscript. DS, TB and EL edited the manuscript. All authors read and approved the final manuscript.

Correspondence to Cindy Rouet.

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Communicated by Herman J. van Eck.

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Rouet, C., Lee, E.A., Banks, T. et al. Identification of a polymorphism within the Rosa multiflora muRdr1A gene linked to resistance to multiple races of Diplocarpon rosae W. in tetraploid garden roses (Rosa × hybrida). Theor Appl Genet 133, 103–117 (2020). https://doi.org/10.1007/s00122-019-03443-9

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Keywords

  • Tetraploid
  • Genotyping-by-sequencing (GBS)
  • Single nucleotide polymorphism (SNP)
  • High-density genetic map
  • Marker-assisted breeding
  • Disease resistance