Identification of a Sidwf1 gene controlling short internode length trait in the sesame dwarf mutant dw607
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SiDWF1 encodes a gibberellin receptor GID1B-like protein controlling the internode length and plant height in sesame.
Sesame is a high-height crop. Here we systematically analyzed the morphological and genetic characters of the sesame dwarf mutant dw607 (dwf1 type). The plant height and the internode length of dw607 significantly declined, while the thousand seed weight (TSW) significantly increased (P < 0.01). The cell size of stem parenchyma and pith tissue reduced, and vascular bundle cells and parenchyma tissue arranged much tighter in the dwarf mutant. Based on the cross-population association mapping of a RIL population of the cross ‘dw607 (dwf1) × 15N41 (wt type),’ the target interval linked to the short internode length was located on C9.scaffold2 of SiChr.4 in sesame. We further screened the 58 variants using the genomic variant data of 824 germplasm and BSA DNA pools and determined the target gene Sidwf1. The SNP mutation of C1057 to T1057 resulted in the amino acid change of P150 (proline) to S150 (serine) in SiDWF1. SiDWF1 gene allele is 1,638 bp and encodes a gibberellin receptor GID1B-like protein. Transcription profile assay reflected that Sidwf1 is highly expressed in leaf, stem, bud, and capsule tissues. The dynamic variation in endogenous GA3 content in dw607 and the wild type was also monitored in this study. The results revealed the molecular genetic mechanism of the internode length and plant height trait in sesame for the first time. The findings supply the theoretical and material basis for developing the marker-assisted selection (MAS) breeding in sesame.
Bulked segregant analysis
Days after sowing
Enzyme-linked immunosorbent assay
Formalin–glacial acetic acid–alcohol
Gibberellin-insensitive dwarf 1
General linear model
Genome-wide association studies
Kyoto Encyclopedia of Genes and Genomes
Next-generation sequencing technology
Quantitative real-time PCR
Quantitative trait loci
Recombinant inbred line
Thousand seed weight
Vascular bundle cells
This work was supported by the Key Laboratory of Specific Oilseed Crops Genomics of Henan Province. This work was financially supported by the earmarked fund for China Agriculture Research System (CARS-14), the Plan for Scientific Innovation Talent of Henan Province (184200510002), the Key Project of Science and Technology in Henan Province (151100111200), the Henan Province Specific Professor Position Program (SPPP2016), the Distinguished Professor Program of Institutions of Higher Learning in Henan Province (DPPIHL2017), the Innovation Scientists and Technicians Troop Construction Projects of Henan Province (ISTTCPHP2016), the Henan Natural Science Foundation (162300410159), and the International Cooperation and Exchanges Project of Henan Province (182102410040).
ZH conceived the technical route and guided the manuscript for publishing. MH guided the experiments, performed the data analysis, and drafted the manuscript. LC and DY conducted the main data analysis and experiments. WL and JM performed the genetic experiments and participated in result validation. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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