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Theoretical and Applied Genetics

, Volume 132, Issue 12, pp 3265–3276 | Cite as

Recombination between homoeologous chromosomes induced in durum wheat by the Aegilops speltoides Su1-Ph1 suppressor

  • Hao Li
  • Le Wang
  • Ming-Cheng Luo
  • Fang Nie
  • Yun Zhou
  • Patrick E. McGuire
  • Assaf Distelfeld
  • Xiongtao Dai
  • Chun-Peng Song
  • Jan DvorakEmail author
Original Article

Abstract

Key message

Su1-Ph1, which we previously introgressed into wheat from Aegilops speltoides, is a potent suppressor of Ph1 and a valuable tool for gene introgression in tetraploid wheat.

Abstract

We previously introgressed Su1-Ph1, a suppressor of the wheat Ph1 gene, from Aegilops speltoides into durum wheat cv Langdon (LDN). Here, we evaluated the utility of the introgressed suppressor for inducing introgression of alien germplasm into durum wheat. We built LDN plants heterozygous for Su1-Ph1 that simultaneously contained a single LDN chromosome 5B and a single Ae. searsii chromosome 5Sse, which targeted them for recombination. We genotyped 28 BC1F1 and 84 F2 progeny with the wheat 90-K Illumina single-nucleotide polymorphism assay and detected extensive recombination between the two chromosomes, which we confirmed by non-denaturing fluorescence in situ hybridization (ND-FISH). We constructed BC1F1 and F2 genetic maps that were 65.31 and 63.71 cM long, respectively. Recombination rates between the 5B and 5Sse chromosomes were double the expected rate computed from their meiotic pairing, which we attributed to selection against aneuploid gametes. Recombination rate between 5B and 5Sse was depressed compared to that between 5B chromosomes in the proximal region of the long arm. We integrated ND-FISH signals into the genetic map and constructed a physical map, which we used to map a 172,188,453-bp Ph1 region. Despite the location of the region in a low-recombination region of the 5B chromosome, we detected three crossovers in it. Our data show that Su1-Ph1 is a valuable tool for gene introgression and gene mapping based on recombination between homoeologous chromosomes in wheat.

Notes

Acknowledgments

We thank Moshe Feldman (Weizmann Institute of Science, Rehovot Area, Israel) for providing Ae. searsii accession TE10. We also thank anonymous reviewers for reading the manuscript and valuable suggestions.

Author Contribution statement

HL, JD, PEM, and AD conceived and designed the experiments. HL, LW, M-CL, constructed genetic maps, HL, FN, YZ, and CS performed HD-FISH and analyzed results, and JD, HL, M-CL, PEM, and AD discussed the findings and interpreted the results. JD and HL wrote the first draft of the paper. All authors have read and approved the final draft.

Funding

This work was supported in part by the China Scholarship Council, the US National Science Foundation (Grants IOS1212591 and IOS1238231), the USDA Grant 2006-01161, the USDA NIFA Hatch Program 1002302, and the US-Israel BARD Project (Grant IS-4829-15).

Complianace with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2019_3423_MOESM1_ESM.xlsx (41 kb)
Supplementary file1 (XLSX 41 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019
corrected publication 2019

Authors and Affiliations

  • Hao Li
    • 1
    • 2
  • Le Wang
    • 2
  • Ming-Cheng Luo
    • 2
  • Fang Nie
    • 1
  • Yun Zhou
    • 1
  • Patrick E. McGuire
    • 2
  • Assaf Distelfeld
    • 3
  • Xiongtao Dai
    • 4
  • Chun-Peng Song
    • 1
  • Jan Dvorak
    • 2
    Email author
  1. 1.Key Laboratory of Plant Stress Biology, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life SciencesHenan UniversityKaifengChina
  2. 2.Department of Plant SciencesUniversity of CaliforniaDavisUSA
  3. 3.School of Plant Sciences and Food SecurityTel Aviv UniversityTel AvivIsrael
  4. 4.Department of StatisticsIowa State UniversityAmesUSA

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