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Effects of Rht-B1 and Ppd-D1 loci on pollinator traits in wheat

  • Takashi OkadaEmail author
  • J. E. A. Ridma M. Jayasinghe
  • Paul Eckermann
  • Nathan S. Watson-Haigh
  • Patricia Warner
  • Yonina Hendrikse
  • Mathieu Baes
  • Elise J. Tucker
  • Hamid Laga
  • Kenji Kato
  • Marc Albertsen
  • Petra Wolters
  • Delphine Fleury
  • Ute Baumann
  • Ryan Whitford
Original Article

Abstract

Key message

Elite wheat pollinators are critical for successful hybrid breeding. We identified Rht-B1 and Ppd-D1 loci affecting multiple pollinator traits and therefore represent major targets for improving hybrid seed production.

Abstract

Hybrid breeding has a great potential to significantly boost wheat yields. Ideal male pollinators would be taller in stature, contain many spikelets well-spaced along the spike and exhibit high extrusion of large anthers. Most importantly, flowering time would match with that of the female parent. Available genetic resources for developing an elite wheat pollinator are limited, and the genetic basis for many of these traits is largely unknown. Here, we report on the genetic analysis of pollinator traits using biparental mapping populations. We identified two anther extrusion QTLs of medium effect, one on chromosome 1BL and the other on 4BS coinciding with the semi-dwarfing Rht-B1 locus. The effect of Rht-B1 alleles on anther extrusion is genotype dependent, while tall plant Rht-B1a allele is consistently associated with large anthers. Multiple QTLs were identified at the Ppd-D1 locus for anther length, spikelet number and spike length, with the photoperiod-sensitive Ppd-D1b allele associated with favourable pollinator traits in the populations studied. We also demonstrated that homeoloci, Rht-D1 and Ppd-B1, influence anther length among other traits. These results suggest that combinations of Rht-B1 and Ppd-D1 alleles control multiple pollinator traits and should be major targets of hybrid wheat breeding programs.

Notes

Acknowledgements

This research was supported by DuPont—Pioneer Hi-Bred International. We thank David Correia, Yuriy Onyskiv, Vy Nguyen, Alex Kovalchuk and Dr Ursula Langridge for assisting with glasshouse work. We also thank Drs Radoslaw Suchecki and Beata Sznajder for data analysis and Dr. Ajay Sandhu for critical advice for the project. We also thank Margaret Pallotta for technical advice and critical reading and editing of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The authors declare that this study complies with the current laws of the countries in which the experiments were performed.

Supplementary material

122_2019_3329_MOESM1_ESM.pdf (545 kb)
Effect of severe dwarf (SD) on pollinator traits and data analysis including SD plants (PDF 546 kb)
122_2019_3329_MOESM2_ESM.pdf (1.2 mb)
Supplemental Figures S1–S9 (PDF 1251 kb)
122_2019_3329_MOESM3_ESM.xlsx (483 kb)
Table S1. KASPTM markers used for developing genetic linkage map for populations #1 and #2. Table S2. GBS markers used for developing genetic linkage map for population #1. Table S3. GBS markers used for developing genetic linkage map for population #2. Table S4. Summary information for genetic linkage map and QTL analysis for population #1 and #2. Table S5. A list of genetic loci associated with anther extrusion reported in the previous publications. Table S6. Phenology genes mapped on Chinese Spring reference sequence IWGSC RefSeq v1.0 to compare physical location of genetic loci associated with pollinator traits. Table S7. Summary statistics for evaluated traits in Rht-1 and Ppd-1 NILs used in this study. Table S8. Physical location of AE loci and flowering time (FT) or plant/floral architecture (FA) genes/loci in CS reference map IWGSC RefSeq v1.0 (XLSX 483 kb)

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Authors and Affiliations

  1. 1.School of Agriculture, Food and Wine, Plant Genomics CentreUniversity of AdelaideUrrbraeAustralia
  2. 2.College of Science, Health, Engineering and EducationMurdoch UniversityMurdochAustralia
  3. 3.Phenomics and Bioinformatics Research CentreUniversity of South AustraliaMawson LakesAustralia
  4. 4.Graduate School of Environmental and Life ScienceOkayama UniversityOkayamaJapan
  5. 5.DuPont-Pioneer Hi-Bred International Inc.JohnstonUSA

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