The Mendelian locus conferring resistance to powdery mildew in soybean was precisely mapped using a combination of phenotypic screening, genetic analyses, and high-throughput genome-wide sequencing.
Powdery mildew (PMD), caused by the fungus Microsphaera diffusa Cooke & Peck, leads to considerable yield losses in soybean [Glycine max (L.) Merr.] under favourable environmental conditions and can be controlled by identifying germplasm resources with resistance genes. In this study, resistance to M. diffusa among resistant varieties B3, Fudou234, and B13 is mapped as a single Mendelian locus using three mapping populations derived from crossing susceptible with resistant cultivars. The position of the PMD resistance locus in B3 is located between simple sequence repeat (SSR) markers GMES6959 and Satt_393 on chromosome 16, at genetic distances of 7.1 cM and 4.6 cM, respectively. To more finely map the PMD resistance gene, a high-density genetic map was constructed using 248 F8 recombinant inbred lines derived from a cross of Guizao1 × B13. The final map includes 3748 bins and is 3031.9 cM in length, with an average distance of 0.81 cM between adjacent markers. This genotypic analysis resulted in the precise delineation of the B13 PMD resistance locus to a 188.06-kb genomic region on chromosome 16 that harbours 28 genes, including 17 disease resistance (R)-like genes in the reference Williams 82 genome. Quantitative real-time PCR assays of possible candidate genes revealed differences in the expression levels of 9 R-like genes between the resistant and susceptible parents. These results provide useful information for marker-assisted breeding and gene cloning for PMD resistance.
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Bulk segregation analysis
Composite interval mapping method
Multiplexed shotgun genotyping
- M. diffusa :
Quantitative trait locus
Recombinant inbred line
Specific length amplified fragment sequencing
Single nucleotide polymorphism
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This work was supported by the National Key R&D Program of China (2017YFD0101500); the Science and Technology Projects of Guangzhou City (201804020015); the China Agricultural Research System (CARS-04-PS09); and the Research Project of the State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics (4100-M13024).
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Communicated by Brian Diers.
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Supplementary Fig. 4: Twenty linkage groups of the soybean high-density genetic map. The high-density bin linkage map was constructed covering 3032 cM, with an average distance of 0.81 cM between adjacent bins. The bin markers and their locations are shown on the right and left sides, respectively (PDF 256 kb)
Supplementary Fig. 6: Results of Rmd-B13 locus analysis using composite Interval Mapping (CIM) method in the F8-derived RILs from a cross of Guizao1 × B13. a The LOD value distribution on the whole genome of the RIL population. b The LOD value distribution on Chr. 16 of the RIL population. c Rmd_B13 is amplified at the site of bin203 on Chr. 16, which explained 78.4% of the phenotypic variance (EPS 115853 kb)
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Jiang, B., Li, M., Cheng, Y. et al. Genetic mapping of powdery mildew resistance genes in soybean by high-throughput genome-wide sequencing. Theor Appl Genet 132, 1833–1845 (2019). https://doi.org/10.1007/s00122-019-03319-y