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Mapping versatile QTL for soybean downy mildew resistance

  • Fumio Taguchi-Shiobara
  • Kenichiro Fujii
  • Takashi Sayama
  • Kaori Hirata
  • Shin Kato
  • Akio Kikuchi
  • Koji Takahashi
  • Masao Iwahashi
  • Chiaki Ikeda
  • Kazuma Kosuge
  • Katsunori Okano
  • Masahiro Hayasaka
  • Yasutaka Tsubokura
  • Masao Ishimoto
Original Article

Abstract

Key message

Three versatile QTL for soybean downy mildew resistance in Japan were detected using five RIL populations and confirmed using recombinant fixed pairs or a backcrossed line.

Abstract

Downy mildew reduces soybean seed quality and size. It is a problem in Japan, where 90% of soybean grown is used as food. In the USA, 33 downy mildew races have been reported, but race differentiation in Japan is unclear. To identify quantitative trait loci (QTL) for downy mildew resistance effective in the Kanto and Tohoku regions, we performed QTL analysis using five populations of recombinant inbred lines (RILs) originated from ‘Natto-shoryu’ × ‘Tachinagaha’ (NT), ‘Natto-shoryu’ × ‘Suzumaru’, ‘Satonohohoemi’ × ‘Fukuibuki’ (SF), ‘Kinusayaka’ × ‘COL/Akita/2009/TARC/1,’ and ‘YR-82’ × ‘Harosoy’ over a 4-year period (2014–2017). We evaluated spontaneously developed symptoms of the RILs and applied 112–233 polymorphic markers to each population. Out of 31 QTL detected, we found five on chromosome 3 in three populations and another five on chromosome 7 in three populations. Other QTL were detected in one population, nine of them in different years. In the NT population, two QTL were detected in a 3.0-Mb region on chromosome 7 and in an 8.1-Mb region on chromosome 18 by evaluating nine recombinant fixed pairs in both Kanto and Tohoku regions. In the SF population, a QTL on chromosome 8 was detected in both regions. This QTL was introduced into the ‘Satonohohoemi’ background by backcrossing, and its effect was confirmed in both regions. In summary, two QTL on chromosomes 7 and 18 from the NT population and one QTL on chromosome 8 from the SF population were confirmed to be effective in both Tohoku and Kanto regions.

Notes

Acknowledgements

This study was supported by Grant-in-Aid for Scientific Research (C) from Japan Society for the Promotion of Science (JSPS KAKENHI Grant Number JP16K07565).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 1 (PDF 40 kb)
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Supplementary material 2 (PDF 106 kb)
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Supplementary material 3 (PDF 57 kb)
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Supplementary material 4 (PDF 61 kb)
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Supplementary material 5 (PDF 64 kb)
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Supplementary material 6 (PDF 75 kb)
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Supplementary material 7 (PDF 63 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Fumio Taguchi-Shiobara
    • 1
  • Kenichiro Fujii
    • 1
  • Takashi Sayama
    • 1
    • 8
  • Kaori Hirata
    • 2
  • Shin Kato
    • 2
  • Akio Kikuchi
    • 2
  • Koji Takahashi
    • 3
  • Masao Iwahashi
    • 4
  • Chiaki Ikeda
    • 4
  • Kazuma Kosuge
    • 4
    • 9
  • Katsunori Okano
    • 4
    • 10
  • Masahiro Hayasaka
    • 5
  • Yasutaka Tsubokura
    • 6
  • Masao Ishimoto
    • 7
  1. 1.Soybean and Field Crop Applied Genomics Research Unit, Institute of Crop ScienceNational Agricultural and Food Research Organization (NARO)TsukubaJapan
  2. 2.Soybean Breeding Group, Tohoku Agricultural Research CenterNational Agricultural and Food Research Organization (NARO)DaisenJapan
  3. 3.Soybean Breeding Group, Institute of Crop ScienceNational Agricultural and Food Research Organization (NARO)TsukubaJapan
  4. 4.Ibaraki Agricultural CenterPlant Biotechnology InstituteMitoJapan
  5. 5.Hokkaido Research FarmSnow Brand Seed Co., Ltd.YubariJapan
  6. 6.Chiba Research FarmSnow Brand Seed Co., Ltd.ChibaJapan
  7. 7.Division of Basic Research, Institute of Crop ScienceNational Agricultural and Food Research Organization (NARO)TsukubaJapan
  8. 8.Shikoku Research Station, Western Region Agricultural Research CenterNational Agricultural and Food Research Organization (NARO)ZentsujiJapan
  9. 9.Namegata District Agricultural Management Guidance CenterRokko Agricultural and Forestry Management OfficeNamegataJapan
  10. 10.Agricultural Management Division, Department of Agriculture, Forestry and FisheriesIbaraki Prefectural governmentMitoJapan

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