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Theoretical and Applied Genetics

, Volume 132, Issue 4, pp 895–906 | Cite as

Independent activation of the BoMYB2 gene leading to purple traits in Brassica oleracea

  • Chenghuan Yan
  • Guanghui An
  • Ting Zhu
  • Weiyi Zhang
  • Lei Zhang
  • Liying Peng
  • Jiongjiong Chen
  • Hanhui KuangEmail author
Original Article
  • 298 Downloads

Abstract

Key message

Transposon insertion and point mutation independently activated the BoMYB2 gene in three purple cultivars of Brassica oleracea including kale, kohlrabi, and cabbage.

Abstract

Several varieties of B. oleracea have both green and purple cultivars. In this study, the causal genes for the purple traits in kale, kohlrabi and cabbage were cloned using map-based cloning approach. The purple traits in all three varieties were mapped to the same locus as the BoMYB2 gene in cauliflower. Surprisingly, the insertion of Harbinger transposon of BoMYB2 in cauliflower was not found in purple kale, kohlrabi and cabbage. Sequencing of the BoMYB2 gene in purple kale and purple kohlrabi discovered a 7606 bp CACTA-like transposon in its promoter region. Transient assay and promoter activity study showed that the insertion upregulated the expression of the BoMYB2 gene. On the other hand, the activation of the BoMYB2 gene in purple cabbage was caused by point mutation and/or 1-bp insertion in its promoter region. Sequence analysis of the BoMYB2 gene in different varieties suggested that the activating events most likely occurred independently after the divergence of cabbage, cauliflower, and kale/kohlrabi. Our results not only contribute to a better understanding of anthocyanin inheritance in B. oleracea, but also provide useful information for future hybrid breeding of purple cultivars through combination of different functional alleles of the BoMYB2 gene.

Notes

Acknowledgements

We thank Dr. Xiuxin Deng (Huazhong Agricultural University) for providing the vector of pGreenII0800 and pCAMBIA1301.

Funding information

This work was supported by National Natural Science Foundation of China [31471162] and [31572131] and the Fundamental Research Funds for the Central Universities [2662015PY084].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2018_3245_MOESM1_ESM.docx (2.6 mb)
Supplementary material 1 (DOCX 2654 kb)
122_2018_3245_MOESM2_ESM.xlsx (30 kb)
Supplementary material 2 (XLSX 30 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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