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Theoretical and Applied Genetics

, Volume 132, Issue 1, pp 257–272 | Cite as

Development and molecular cytogenetic identification of a new wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust and sharp eyespot

  • Diaoguo AnEmail author
  • Pengtao Ma
  • Qi Zheng
  • Shulan Fu
  • Lihui Li
  • Fangpu Han
  • Guohao Han
  • Jing Wang
  • Yunfeng Xu
  • Yuli Jin
  • Qiaoling Luo
  • Xiaotian Zhang
Original Article

Abstract

Key message

A wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust, sharp eyespot and high kernel number per spike was developed and characterized by molecular cytogenetic method as novel resistant germplasm.

Abstract

Rye (Secale cereale L.), a close relative of common wheat, is an important and valuable gene donor with multiple disease resistance for wheat improvement. However, resistance genes derived from rye have successively lost resistance to pathogens due to the coevolution of pathogen virulence and host resistance. Development and identification of new effective resistance gene sources from rye therefore are of special importance and urgency. In the present study, a wheat-rye line WR35 was produced through distant hybridization, embryo rescue culture, chromosome doubling and backcrossing. WR35 was then proven to be a new wheat-rye 4R disomic addition line using sequential GISH (genomic in situ hybridization), mc-FISH (multicolor fluorescence in situ hybridization) and ND-FISH (non-denaturing FISH) with multiple probes, mc-GISH (multicolor GISH), rye chromosome arm-specific marker analysis and SLAF-seq (specific-locus amplified fragment sequencing) analysis. At the adult stage, WR35 exhibited high levels of resistance to the powdery mildew (Blumeria graminis f. sp. tritici, Bgt) and stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens prevalent in China, and a highly virulent isolate of Rhizoctonia cerealis, the cause of wheat sharp eyespot. At the seedling stage, it was highly resistant to 22 of 23 Bgt isolates and four Pst races. Based on its disease responses to different pathogen isolates, WR35 may possess resistance gene(s) for powdery mildew, stripe rust and sharp eyespot, which differed from the known resistance genes from rye. In addition, WR35 was cytologically stable and produced high kernel number per spike. Therefore, WR35 with multi-disease resistances and desirable agronomic traits should serve as a promising bridging parent for wheat chromosome engineering breeding.

Abbreviations

Bgt

Blumeria graminis f. sp. tritici

CS

Chinese Spring wheat

DAPI

4,6-Diamidino-2-phenylindole

EST-SSR

Expressed sequence tag-simple sequence repeat

FISH

Fluorescence in situ hybridization

GISH

Genomic in situ hybridization

IT

Infection type

mc-FISH

Multicolor FISH

ND-FISH

Non-denaturing FISH

SLAF

Specific-locus amplified fragment sequencing

PCR

Polymerase chain reaction

Pst

Puccinia striiformis f. sp. tritici

Notes

Acknowledgements

The authors thank Dr. Yilin Zhou and Dr. Shichang Xu from the State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, and Dr. Shibin Cai from Institute of Food Crops, Jiangsu Academy of Agricultural Science, Nanjing, China, for conducting assessment of the reactions to powdery mildew, stripe rust and sharp eyespot. This research was supported by the National Key Research and Development Program of China (Nos. 2016YFD0102002 and 2016YFD0100102) and the National Natural Science Foundation of China (No. 31771793).

Compliance with ethical standards

Conflict of interest

The authors declare that our experiments comply with the current laws of China and we have no conflicts of interest.

Ethical approval

This article does not contain any studies that were performed with human participants or animals by any of the authors.

Supplementary material

122_2018_3214_MOESM1_ESM.pdf (150 kb)
Supplementary material 1 (PDF 149 kb)
122_2018_3214_MOESM2_ESM.pdf (20 kb)
Supplementary material 2 (PDF 19 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Center for Agricultural Resources Research, Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuangChina
  2. 2.The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  3. 3.The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina
  4. 4.Province Key Laboratory of Plant Breeding and GeneticsSichuan Agriculture UniversityChengduChina

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