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Theoretical and Applied Genetics

, Volume 132, Issue 1, pp 217–225 | Cite as

Development of tightly linked markers and identification of candidate genes for Fusarium crown rot resistance in barley by exploiting a near-isogenic line-derived population

  • Yunfeng Jiang
  • Ahsan Habib
  • Zhi Zheng
  • Meixue Zhou
  • Yuming Wei
  • You-Liang Zheng
  • Chunji LiuEmail author
Original Article

Abstract

Key message

This study demonstrates the feasibility of developing co-segregating markers and identifying candidate genes for Fusarium crown rot resistance in barley based on the generation and exploitation of a near-isogenic line-derived large population.

Abstract

Fusarium crown rot (FCR) is a chronic and severe disease in cereals in semi-arid regions worldwide. Previous studies showed that FCR assessment could be affected by many factors including plant height, growth rate as well as drought stress. Thus, accurate assessment, which is essential for detailed mapping of any locus conferring FCR resistance, is difficult. Targeting one of the resistance loci reported earlier, we developed a near-isogenic line-derived population consisting of 1820 F9 lines. By analysing this population, the Qcrs.cpi-4H locus was mapped to an interval of 0.09 cM covering a physical distance of about 637 kb and 13 markers co-segregating with the targeted locus were developed. Candidate genes underlying the resistance locus were identified by analysing the expression and sequence variation of genes in the targeted interval. The accurate localization and the development of co-segregating markers should facilitate the incorporation of this large-effect QTL into breeding programmes as well as the cloning of gene(s) underlying the locus.

Notes

Acknowledgements

The work reported here was supported by the Commonwealth Scientific and Industrial Organization (CSIRO), Australia (Project code: R-10191-01). YJ is grateful to the Sichuan Agricultural University and the China Scholarship Council for funding his visit to CSIRO. AH is grateful to University of Tasmania, Australia, and Khulna University, Bangladesh, for financial supports during the tenure of his PhD studentship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2018_3209_MOESM1_ESM.pdf (424 kb)
Supplementary material 1 (PDF 423 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Commonwealth Scientific and Industrial Research Organisation, Agriculture and FoodSt. LuciaAustralia
  2. 2.Triticeae Research InstituteSichuan Agricultural UniversityWenjiang, ChengduChina
  3. 3.School of Land and Food and Tasmanian Institute of AgricultureUniversity of TasmaniaHobartAustralia
  4. 4.Biotechnology and Genetic Engineering DisciplineKhulna UniversityKhulnaBangladesh
  5. 5.National Foxtail Millet Improvement Centre, Institute of Millet CropsHebei Academy of Agricultural and Forestry SciencesShijiazhuangChina

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