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Theoretical and Applied Genetics

, Volume 132, Issue 1, pp 205–216 | Cite as

Organelle DNA contents and starch accumulation in potato tubers

  • Suyan Niu
  • Guodong Zhang
  • Xiubao Li
  • Muhammad Haroon
  • Huaijun Si
  • Guoqiang Fan
  • Xiu-Qing Li
Original Article

Abstract

Key message

Starch contents were found to be positively correlated with organelle/nuclear DNA ratios, suggesting that these ratios are involved in starch accumulation and may serve as a target trait in genetic engineering and a biomarker in breeding for improving the dry matter and starch production in potato.

Abstract

Starch is the main dry matter component of various staple food crops, including potato. Starch synthesis and accumulation is in plastids, uses sugar, consumes cellular energy, and requires active expression of starch synthesis genes. We hypothesized that the plastid/nuclear DNA ratios and mitochondrial/nuclear DNA ratios are involved in this accumulation. We analyzed the dry mater, starch, plastid DNA, mitochondrial DNA, and nuclear DNA in tuber stem ends and tuber bud ends in two potato cultivars and verified the results using whole tubers in nine potato cultivars. Dry matter contents (DMC) and organelle/nuclear DNA ratios increased rapidly during tuber bulking. DMC and starch contents were greater at the tuber stem ends than at the tuber bud ends. Both the comparisons between tuber ends and among whole tubers indicated that DMC and starch contents were positively correlated with both plastid/nuclear DNA ratios and mitochondrial/nuclear DNA ratios. The results suggest that pt/nuc and mt/nuc DNA ratios are important and may serve as a biomarker in selection, genetic engineering, and cytoplasm manipulation, for dry matter and starch accumulation in potato.

Notes

Acknowledgements

We thank the China Scholarship Council for its support to GDZ, the greenhouse management team of the Fredericton Research and Development Centre of Agriculture and Agri-Food Canada for the support in growing the potato plants. The research was supported by A-base funding from Agriculture and Agri-Food Canada to XQL.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Crown 2018

Authors and Affiliations

  1. 1.Institute of BioengineeringZhengzhou Normal UniversityZhengzhouChina
  2. 2.Fredericton Research and Development CentreAgriculture and Agri-Food CanadaFrederictonCanada
  3. 3.College of ForestHenan Agricultural UniversityZhengzhouChina
  4. 4.Gansu Provincial Key Laboratory of Arid land Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm EnhancementGansu Agricultural UniversityLanzhouChina
  5. 5.College of AgronomyGansu Agricultural UniversityLanzhouChina
  6. 6.Rizhao Academy of Agricultural SciencesRizhaoChina
  7. 7.College of Life Science and TechnologyGansu Agricultural UniversityLanzhouChina

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