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Theoretical and Applied Genetics

, Volume 132, Issue 1, pp 163–175 | Cite as

Development and characterization of wheat–sea wheatgrass (Thinopyrum junceiforme) amphiploids for biotic stress resistance and abiotic stress tolerance

  • Wanlong LiEmail author
  • Qijun Zhang
  • Shuwen Wang
  • Marie A. Langham
  • Dilkaran Singh
  • Robert L. Bowden
  • Steven S. Xu
Original Article

Abstract

Key message

Development of a complete wheat–Thinopyrum junceiforme amphiploid facilitated identification of resistance to multiple pests and abiotic stress derived from the wild species and shed new light on its genome composition.

Abstract

Wheat production is facing numerous challenges from biotic and abiotic stresses. Alien gene transfer has been an effective approach for wheat germplasm enhancement. Thinopyrum junceiforme, also known as sea wheatgrass (SWG), is a distant relative of wheat and a relatively untapped source for wheat improvement. In the present study, we developed a complete amphiploid, 13G819, between emmer wheat and SWG for the first time. Analysis of the chromosome constitution of the wheat–SWG amphiploid by multiple-color genomic in situ hybridization indicated that SWG is an allotetraploid with its J1 genome closely related to Th. bessarabicum and Th. elongatum, and its J2 genome was derived from an unknown source. Two SWG-derived perennial wheat lines, 14F3516 and 14F3536, are partial amphiploids and carry 13 SWG chromosomes of mixed J1 and J2 genome composition, suggesting cytological instability. We challenged the amphiploid 13G819 with various abiotic and biotic stress treatments together with its emmer wheat parent. Compared to its emmer wheat parent, the amphiploid showed high tolerance to waterlogging, manganese toxicity and salinity, low nitrogen and possibly to heat as well. The amphiploid 13G819 is also highly resistant to the wheat streak mosaic virus (temperature insensitive) and Fusarium head blight. All three amphiploids had solid stems, which confer resistance to wheat stem sawflies. All these traits make SWG an excellent source for improving wheat resistance to diseases and insects and tolerance to abiotic stress.

Notes

Acknowledgements

We thank Drs. Harold Bockelman and David Stout for supplying the seeds and Dr. Ghana Challa for technical assistance in setting up the hydroponic system. This work is supported by a Grant from the USDA AFRI Plant Breeding Program (2017-67014-26210).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2018_3205_MOESM1_ESM.pptx (26.3 mb)
Supplementary material 1 (PPTX 26941 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biology and MicrobiologySouth Dakota State UniversityBrookingsUSA
  2. 2.Department of Agronomy, Horticulture and Plant ScienceSouth Dakota State UniversityBrookingsUSA
  3. 3.Department of Plant ScienceNorth Dakota State UniversityFargoUSA
  4. 4.The Land InstituteSalinaUSA
  5. 5.USDA-ARS, Hard Winter Wheat Genetics Research UnitManhattanUSA
  6. 6.Cereal Crops Research Unit, Red River Valley Agricultural Research CenterUSDA-ARSFargoUSA

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