Construction of a high-density linkage map and QTL detection of downy mildew resistance in Vitis aestivalis-derived ‘Norton’
- 539 Downloads
A major QTL for downy mildew resistance was detected on chromosome 18 (Rpv27) in Vitis aestivalis-derived ‘Norton’ based on a high-resolution linkage map with SNP and SSR markers as well as 2 years of field and laboratory phenotyping data.
Grapevine downy mildew caused by the oomycete Plasmopara viticola is one of the most widespread and destructive diseases, particularly in humid viticultural areas where it damages green tissues and defoliates vines. Traditional Vitis vinifera wine grape cultivars are susceptible to downy mildew whereas several North American and a few Asian cultivars possess various levels of resistance to this disease. To identify genetic determinants of downy mildew resistance in V. aestivalis-derived ‘Norton,’ a mapping population with 182 genotypes was developed from a cross between ‘Norton’ and V. vinifera ‘Cabernet Sauvignon’ from which a consensus map was constructed via 411 simple sequence repeat (SSR) markers. Using genotyping-by-sequencing, 3825 single nucleotide polymorphism (SNP) markers were also generated. Of these, 1665 SNP and 407 SSR markers were clustered into 19 linkage groups in 159 genotypes, spanning a genetic distance of 2203.5 cM. Disease progression in response to P. viticola was studied in this population for 2 years under both laboratory and field conditions, and strong correlations were observed among data sets (Spearman correlation coefficient = 0.57–0.79). A quantitative trait loci (QTL) analysis indicated a resistance locus on chromosome 18, here named Rpv27, explaining 33.8% of the total phenotypic variation. Flanking markers closely linked with the trait can be further used for marker-assisted selection in the development of new cultivars with resistance to downy mildew.
The authors thank S. Jacob Schneider, Marilyn Odneal and Kevin Fort for valuable discussions and constructive comments on the manuscript. This project was supported by Agriculture and Food Research Initiative Competitive Grant, Award No. 2013-67014-21360, and Specialty Crop Research Initiative Competitive Grant, Award No. 2011-51181-30635, of the USDA National Institute of Food and Agriculture.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Bellin D, Peressotti E, Merdinoglu D, Wiedemann-Merdinoglu S, Adam-Blondon A-F, Cipriani G, Morgante M, Testolin R, Di Gaspero G (2009) Resistance to Plasmopara viticola in grapevine ‘Bianca’ is controlled by a major dominant gene causing localized necrosis at the infection site. Theor Appl Genet 120:163–176CrossRefGoogle Scholar
- Brown MV, Moore JN, Fenn P, McNew RW (1999) Comparison of leaf disk, greenhouse, and field screening procedures for evaluation of grape seedlings for downy mildew resistance. HortScience 34:331–333Google Scholar
- Einset J, Pratt C (1975) Grapes. In: Janick J, Moore JN (eds) Advances in fruit breeding. Purdue University Press, West Lafayette, pp 130–153Google Scholar
- Ganal MW, Polley A, Graner EM, Plieske J, Wieseke R, Luerssen H, Durstewitz G (2012) Large SNP arrays for genotyping in crop plants. J Biol Sci 37:821–828Google Scholar
- Kono A, Sato A, Reisch B, Cadle-Davidson L (2015) Effect of detergent on the quantification of grapevine downy mildew sporangia from leaf discs. HortScience 50:656–660Google Scholar
- Langcake P, Lovell E (1980) Light and electron microscopical studies of the infection of Vitis spp. by Plasmopara viticola, the downy mildew pathogen. Vitis 19:321–337Google Scholar
- Ochssner I, Hausmann L, Töpfer R (2016) Rpv14, a new genetic source for Plasmopara viticola resistance conferred by Vitis cinerea. Vitis 55:79–81Google Scholar
- Polesani M, Bortesi L, Ferrarini A, Zamboni A, Fasoli M, Zadra C, Lovato A, Pezzotti M, Delledonne M, Polverari A (2010) General and species-specific transcriptional responses to downy mildew infection in a susceptible (Vitis vinifera) and a resistant (V. riparia) grapevine species. BMC Genom 11:117CrossRefGoogle Scholar
- Pootakham W, Ruang-Areerate P, Jomchai N, Sonthirod C, Sangsrakru D, Yoocha T, Theerawattanasuk K, Nirapathpongporn K, Romruensukharom P, Tragoonrung S (2015) Construction of a high-density integrated genetic linkage map of rubber tree (Hevea brasiliensis) using genotyping-by-sequencing (GBS). Front Plant Sci 6:367CrossRefGoogle Scholar
- Spindel J, Wright M, Chen C, Cobb J, Gage J, Harrington S, McCouch S (2013) Bridging the genotyping gap: using genotyping by sequencing (GBS) to add high-density SNP markers and new value to traditional bi-parental mapping and breeding populations. Theor Appl Genet 126:2699–2716CrossRefGoogle Scholar
- van Ooijen JW (2006) JoinMap 4, software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, WageningenGoogle Scholar
- van Ooijen JW (2009) MapQTL 6, software for the mapping of quantitative trait loci in experimental populations of diploid species. Kyazma BV, WageningenGoogle Scholar
- VIVC (2018) Table of Loci for Traits in Grapevine Relevant for Breeding and Genetics. http://www.vivc.de/docs/dataonbreeding/20181001_Table%20of%20Loci%20for%20Traits%20in%20Grapevine.pdf
- Wiedemann-Merdinoglu S, Prado E, Coste P, Dumas V, Butterlin G, Bouquet A, Merdinoglu D (2006) Genetic analysis of resistance to downy mildew from Muscadinia rotundifolia. In: 9th international conference on grape genetics and breeding 2006Google Scholar
- Zhou X, Xia Y, Ren X, Chen Y, Huang L, Huang S, Liao B, Lei Y, Yan L, Jiang H (2014) Construction of a SNP-based genetic linkage map in cultivated peanut based on large scale marker development using next-generation double-digest restriction-site-associated DNA sequencing (ddRADseq). BMC Genom 15:351CrossRefGoogle Scholar