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Promoter variations in a homeobox gene, BnA10.LMI1, determine lobed leaves in rapeseed (Brassica napus L.)

  • Limin Hu
  • Hao Zhang
  • Qingyong Yang
  • Qingwei Meng
  • Shaoqing Han
  • Chinedu Charles Nwafor
  • Muhammad Hafeez Ullah Khan
  • Chuchuan Fan
  • Yongming Zhou
Original Article

Abstract

Key message

BnA10.LMI1 positively regulates the development of leaf lobes in Brassica napus, and cis-regulatory divergences cause the different allele effects.

Abstract

Leaf shape is an important agronomic trait, and large variations in this trait exist within the Brassica germplasm. The lobed leaf is a unique morphological characteristic for Brassica improvement. Nevertheless, the molecular basis of leaf lobing in Brassica is poorly understood. Here, we show that an incompletely dominant locus, BnLLA10, is responsible for the lobed-leaf shape in rapeseed. A LATE MERISTEM IDENTITY1 (LMI1)-like gene (BnA10.LMI1) encoding an HD-Zip I transcription factor is the causal gene underlying the BnLLA10 locus. Sequence analysis of parental alleles revealed no sequence variations in the coding sequences, whereas abundant variations were identified in the regulatory region. Consistent with this finding, the expression levels of BnLMI1 were substantially elevated in the lobed-leaf parent compared with its near-isogenic line. The knockout mutations of BnA10.LMI1 gene were induced using the CRISPR/Cas9 system in both HY (the lobed-leaf parent) and J9707 (serrated leaf) genetic backgrounds. BnA10.LMI1 null mutations in the HY background were sufficient to produce unlobed leaves, whereas null mutations in the J9707 background showed no obvious changes in leaf shape compared with the control. Collectively, our results indicate that BnA10.LMI1 positively regulates the development of leaf lobes in B. napus, with cis-regulatory divergences causing the different allelic effects, providing new insights into the molecular mechanism of leaf lobe formation in Brassica crops.

Notes

Acknowledgements

We thank Prof. Yaoguang Liu (South China Agriculture University) for kindly providing the sgRNA-Cas9 plant expression vectors. This study was supported by Grants 2015CB150202 from the National Key Basic Research Program of China and the Fundamental Research Funds for the Central Universities (programme No. 2662015PY172).

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Supplementary material

122_2018_3184_MOESM1_ESM.pdf (1.6 mb)
Supplementary material 1 (PDF 1619 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina
  2. 2.Hubei Key Laboratory of Agricultural Bioinformatics, College of InformaticsHuazhong Agricultural UniversityWuhanChina

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