Resistance to Rhynchosporium commune in a collection of European spring barley germplasm
- 468 Downloads
Association analyses of resistance to Rhynchosporium commune in a collection of European spring barley germplasm detected 17 significant resistance quantitative trait loci. The most significant association was confirmed as Rrs1.
Rhynchosporium commune is a fungal pathogen of barley which causes a highly destructive and economically important disease known as rhynchosporium. Genome-wide association mapping was used to investigate the genetic control of host resistance to R. commune in a collection of predominantly European spring barley accessions. Multi-year disease nursery field trials revealed 8 significant resistance quantitative trait loci (QTL), whilst a separate association mapping analysis using historical data from UK national and recommended list trials identified 9 significant associations. The most significant association identified in both current and historical data sources, collocated with the known position of the major resistance gene Rrs1. Seedling assays with R. commune single-spore isolates expressing the corresponding avirulence protein NIP1 confirmed that this locus is Rrs1. These results highlight the significant and continuing contribution of Rrs1 to host resistance in current elite spring barley germplasm. Varietal height was shown to be negatively correlated with disease severity, and a resistance QTL was identified that co-localised with the semi-dwarfing gene sdw1, previously shown to contribute to disease escape. The remaining QTL represent novel resistances that are present within European spring barley accessions. Associated markers to Rrs1 and other resistance loci, identified in this study, represent a set of tools that can be exploited by breeders for the sustainable deployment of varietal resistance in new cultivars.
We thank the IMPROMALT consortium for making the germplasm collection as well as the genotypic and historical phenotypic data available for this study and the BSPB and the AHDB Cereals and Oilseeds division for the provision of the National and Recommended List data. We also thank Richard Keith, Chris Warden, Dave Guy and Alfred Barth for all their technical help in this work. This work was funded by the Scottish Government Rural and Environment Science and Analytical Services (RESAS) and the Bavarian State Ministry of Food, Agriculture and Forestry and the BMBF under Grant-no 031B0199D. MEL and WTBT were also funded by the BBSRC IMPROMALT project BB/J019593/1. LLG was funded by the BBSRC training Grant (BB/K501906/1). AA was also funded by the BBSRC-CIRC project BB/J019569/1.
Compliance with ethical standards
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Ariyadasa R, Mascher M, Nussbaumer T, Schulte D, Frenkel Z, Poursarebani N, Zhou R, Steuernagel B, Gundlach H, Taudien S, Felder M, Platzer M, Himmelbach A, Schmutzer T, Hedley PE, Muehlbauer GJ, Scholz U, Korol A, Mayer KFX, Waugh R, Langridge P, Graner A, Stein N (2014) A sequence-ready physical map of barley anchored genetically by two million single-nucleotide polymorphisms. Plant Physiol 164:412–423CrossRefGoogle Scholar
- Comadran J, Kilian B, Russell J, Ramsay L, Stein N, Ganal M, Shaw P, Bayer M, Thomas W, Marshall D, Hedley P, Tondelli A, Pecchioni N, Francia E, Korzun V, Walther A, Waugh R (2012) Natural variation in a homolog of Antirrhinum CENTRORADIALIS contributed to spring growth habit and environmental adaptation in cultivated barley. Nat Genet 44:1388–1392CrossRefGoogle Scholar
- Fitt BD, Atkins SD, Fraaije BA, Lucas JA, Newton AC, Looseley ME, Werner P, Harrap D, Ashworth M, Southgate J, Phillips H, Gilchrist A (2010) Role of inoculum sources in Rhynchosporium population dynamics and epidemiology on barley. HGCA final report. Project number RD-2004-3099Google Scholar
- Mascher M, Gundlach H, Himmelbach A, Beier S, Twardziok SO, Wicker T, Radchuk V, Dockter C, Hedley PE, Russell J, Bayer M, Ramsay L, Liu H, Haberer G, Zhang X-Q, Zhang Q, Barrero RA, Li L, Taudien S, Groth M, Felder M, Hastie A, Šimková H, Staňková H, Vrána J, Chan S, Muñoz-Amatriaín M, Ounit R, Wanamaker S, Bolser D, Colmsee C, Schmutzer T, Aliyeva-Schnorr L, Grasso S, Tanskanen J, Chailyan A, Sampath D, Heavens D, Clissold L, Cao S, Chapman B, Dai F, Han Y, Li H, Li X, Lin C, McCooke JK, Tan C, Wang P, Wang S, Yin S, Zhou G, Poland JA, Bellgard MI, Borisjuk L, Houben A, Doležel J, Ayling S, Lonardi S, Kersey P, Langridge P, Muehlbauer GJ, Clark MD, Caccamo M, Schulman AH, Mayer KFX, Platzer M, Close TJ, Scholz U, Hansson M, Zhang G, Braumann I, Spannagl M, Li C, Waugh R, Stein N (2017) A chromosome conformation capture ordered sequence of the barley genome. Nature 544:427–433CrossRefGoogle Scholar
- Muñoz-Amatriaín M, Moscou MJ, Bhat PR, Svensson JT, Bartoš J, Suchánková P, Šimková H, Endo TR, Fenton RD, Lonardi S, Castillo AM, Chao S, Cistué L, Cuesta-Marcos A, Forrest KL, Hayden MJ, Hayes PM, Horsley RD, Makoto K, Moody D, Sato K, Vallés MP, Wulff BBH, Muehlbauer GJ, Doležel J, Close TJ (2011) An improved consensus linkage map of barley based on flow-sorted chromosomes and single nucleotide polymorphism markers. Plant Genome 4:238–249CrossRefGoogle Scholar
- Newton AC, Searle J, Guy DC, Hackett CA, Cooke DEL (2001) Variability in pathotype, aggressiveness, RAPD profile, and rDNA ITS1 sequences of UK isolates of Rhynchosporium secalis. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz-J Plant Dis Protect 108:446–458Google Scholar
- Schweizer G, Herz M, Mikolajewski S, Brenner M, Hartl L, Baumer M (2004) Genetic mapping of a novel scald resistance gene Rrs15CI8288 in barley. In: Proceedings of the 9th international barley genetics symposium, Brno, Czech Republic, pp 258–265Google Scholar
- Thomas WTB, Powell W, Waugh R, Chalmers KJ, Barua UM, Jack P, Lea V, Forster BP, Swanston JS, Ellis RP, Hanson PR, Lance RCM (1995) Detection of quantitative trait loci for agronomic, yield, grain and disease characters in spring barley (Hordeum vulgare L.). Theor Appl Genet 91:1037–1047PubMedGoogle Scholar
- Thomas W, Comadran J, Ramsay L, Shaw P, Marshall D, Newton AC, O’Sullivan DM, Cockram J, Mackay IJ, Bayles R, White J, Kearsey M, Luo Z, Wang M, Tapsell C, Harrap D, Werner P, Klose S, Bury P, Wroth J, Argillier O, Habgood R, Glew M, Bochard A-M, Gymer P, Vequaud D, Christerson T, Allvin B, Davies N, Broadbent R, Brosnan J, Bringhurst T, Booer C, Waugh R (2014) Project Report No. 528: association genetics of UK elite barley (AGOUEB). HGCAGoogle Scholar
- Tondelli A, Xu X, Moragues M, Sharma R, Schnaithmann F, Ingvardsen C, Manninen O, Comadran J, Russell J, Waugh R, Schulman AH, Pillen K, Rasmussen SK, Kilian B, Cattivelli L, Thomas WTB, Flavell AJ (2013) Structural and temporal variation in genetic diversity of European spring two-row barley cultivars and association mapping of quantitative traits. Plant Genome. https://doi.org/10.3835/plantgenome2013.03.0007 CrossRefGoogle Scholar
- VSN International (2011) Genstat for windows, 14th edn. VSN International, Hemel HempsteadGoogle Scholar
- Wagner C, Schweizer G, Kramer M, Dehmer-Badani AG, Ordon F, Friedt W (2008) The complex quantitative barley-Rhynchosporium secalis interaction: newly identified QTL may represent already known resistance genes. Theor Appl Genet 118:113–122. https://doi.org/10.1007/s00122-008-0881-5 CrossRefPubMedGoogle Scholar
- Waugh R, Flavell JA, Russell J, Thomas W, Ramsay L, Comadran J (2014) Exploiting barley genetic resources for genome wide association scans (GWAS). In: Tuberosa R, Graner A, Frison E (eds) Genomics of plant genetic resources, vol 1. Managing, sequencing and mining genetic resources. Springer, Dordrecht, pp 237–254CrossRefGoogle Scholar
- Xu X, Sharma R, Tondelli A, Russell J, Comadran J, Schnaithmann F, Pillen K, Kilian B, Cattivelli L, Thomas WTB, Flavell AJ (2018) Genome-wide association analysis of grain yield-associated traits in a pan-European barley cultivar collection. Plant Genome. https://doi.org/10.3835/plantgenome2017.08.0073 CrossRefPubMedGoogle Scholar
- Zhan J, Fitt BDL, Pinnschmidt HO, Oxley SJP, Newton AC (2008) Resistance, epidemiology and sustainable management of Rhynchosporium secalis populations on barley. Plant Pathol 57:1–14Google Scholar