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Correction to: Development and characterization of a spring hexaploid wheat line with no functional VRN2 genes

  • Nestor Kippes
  • Andrew Chen
  • Xiaoqin Zhang
  • Adam J. Lukaszewski
  • Jorge Dubcovsky
Correction

Correction to: Theor Appl Genet (2016) 129:1417–1428 https://doi.org/10.1007/s00122-016-2713-3

In the original version of this article, PCR fragments and digestion product sizes for the VRN-B2 and VRN-D2 markers were not accurate. The corrected sizes are detailed below:

Primers SNF-B2-3p-F1 and SNFB2-3p-R2 for the SNF-B2 gene tightly linked to VRN-B2 amplified a 1281 bp product. Digestion of the amplified product with restriction enzyme HpyCH4IV yielded two fragments (1041 and 240 bp) for the allele linked to the vrnB2-null deletion and three fragments (633, 408 and 240 bp) for the wild type allele from the hexaploid winter wheat variety Goodstreak.

Primers for the VRN-D2 gene amplified a 687-bp fragment from the functional allele in the hexaploid wheat Goodstreak and a 681-bp fragment from the non-functional allele from Ae. tauschii accession E1. Digestion of the amplified PCR products with the restriction enzyme MboII yielded three fragments (396, 196 and 95 bp) in hexaploid wheat and two fragments (586 and 95 bp) in Ae. tauschii E1.

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Nestor Kippes
    • 1
  • Andrew Chen
    • 1
  • Xiaoqin Zhang
    • 1
  • Adam J. Lukaszewski
    • 2
  • Jorge Dubcovsky
    • 1
    • 3
  1. 1.Department of Plant SciencesUniversity of CaliforniaDavisUSA
  2. 2.Department of Botany and Plant SciencesUniversity of CaliforniaRiversideUSA
  3. 3.Howard Hughes Medical Institute and Gordon and Betty Moor Foundation InvestigatorDavisUSA

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