Mapping and validation of a new QTL for adult-plant resistance to powdery mildew in Chinese elite bread wheat line Zhou8425B
Four QTLs for adult-plant resistance to powdery mildew were mapped in the Zhou8425B/Chinese Spring population, and a new QTL on chromosome 3B was validated in 103 wheat cultivars derived from Zhou8425B.
Zhou8425B is an elite wheat (Triticum aestivum L.) line widely used as a parent in Chinese wheat breeding programs. Identification of genes for adult-plant resistance (APR) to powdery mildew in Zhou8425B is of high importance for continued controlling the disease. In the current study, the high-density Illumina iSelect 90K single-nucleotide polymorphism (SNP) array was used to map quantitative trait loci (QTL) for APR to powdery mildew in 244 recombinant inbred lines derived from the cross Zhou8425B/Chinese Spring. Inclusive composite interval mapping identified QTL on chromosomes 1B, 3B, 4B, and 7D, designated as QPm.caas-1BL.1, QPm.caas-3BS, QPm.caas-4BL.2, and QPm.caas-7DS, respectively. Resistance alleles at the QPm.caas-1BL.1, QPm.caas-3BS, and QPm.caas-4BL.2 loci were contributed by Zhou8425B, whereas that at QPm.caas-7DS was from Chinese Spring. QPm.caas-3BS, likely to be a new APR gene for powdery mildew resistance, was detected in all four environments. One SNP marker closely linked to QPm.caas-3BS was transferred into a semi-thermal asymmetric reverse PCR (STARP) marker and tested on 103 commercial wheat cultivars derived from Zhou8425B. Cultivars with the resistance allele at the QPm.caas-3BS locus had averaged maximum disease severity reduced by 5.3%. This STARP marker can be used for marker-assisted selection in improvement of the level of powdery mildew resistance in wheat breeding.
KeywordsAPR Blumeria tritici f. sp. tritici STARP marker Triticum aestivum Wheat 90K SNP array
Analysis of variance
Best linear unbiased estimates
Cleaved amplified polymorphic sequences
Quantitative trait locus (loci)
Inclusive composite interval mapping
Kompetitive allele-specific PCR
Logarithm of odds
Maximum disease severity
Phenotypic variance explained
Restriction fragment length polymorphism
Recombinant inbred line
Semi-thermal asymmetric reverse PCR
The authors are grateful to Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, for review of this manuscript. This study was supported by the National Key Research and Development Program of China (2016YFD0101802), National Natural Science Foundation of China (31461143021), Natural Science Foundation of Henan province (162300410348), and CAAS Science and Technology Innovation Program.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
We declare that these experiments complied with the ethical standards in China.
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