Theoretical and Applied Genetics

, Volume 131, Issue 4, pp 959–971 | Cite as

The effect of INDEHISCENT point mutations on silique shatter resistance in oilseed rape (Brassica napus)

  • Janina Braatz
  • Hans-Joachim Harloff
  • Nazgol Emrani
  • Chirlon Elisha
  • Lars Heepe
  • Stanislav N. Gorb
  • Christian JungEmail author
Original Article


Key message

This study elucidates the influence of indehiscent mutations on rapeseed silique shatter resistance. A phenotype with enlarged replum-valve joint area and altered cell dimensions in the dehiscence zone is described.


Silique shattering is a major factor reducing the yield stability of oilseed rape (Brassica napus). Attempts to improve shatter resistance often include the use of mutations in target genes identified from Arabidopsis (Arabidopsis thaliana). A variety of phenotyping methods assessing the level of shatter resistance were previously described. However, a comparative and comprehensive evaluation of the methods has not yet been undertaken. We verified the increase of shatter resistance in indehiscent double knock-down mutants obtained by TILLING with a systematic approach comparing three independent phenotyping methods. A positive correlation of silique length and shatter resistance was observed and accounted for in the analyses. Microscopic studies ruled out the influence of different lignification patterns. Instead, we propose a model to explain increased shattering resistance of indehiscent rapeseed mutants by altered cell shapes and sizes within the contact surfaces of replum and valves.



This study was financed by the Stiftung Schleswig-Holsteinische Landschaft under Grant no. 2013/69. We thank Monika Bruisch, Hilke Jensen, Lara Wostupatsch, and Niloufar Nezaratizadeh for technical assistance; Mario Hasler for support on statistical analyses; Maria Mulisch and Cay Kruse for assistance with light microscopy of cryosections; the Institute of Clinical Molecular Biology in Kiel for Sanger sequencing; the breeding company Norddeutsche Pflanzenzucht Hans-Georg Lembke for collaboration regarding the EMS mutant population.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2018_3051_MOESM1_ESM.pptx (6.8 mb)
Supplementary material 1 (PPTX 6917 kb)
122_2018_3051_MOESM2_ESM.docx (29 kb)
Supplementary material 2 (DOCX 30 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Plant Breeding Institute, Christian-Albrechts-University of KielKielGermany
  2. 2.Zoological Institute, Functional Morphology and BiomechanicsChristian-Albrechts-University of KielKielGermany

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