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Theoretical and Applied Genetics

, Volume 131, Issue 2, pp 377–388 | Cite as

Development of a complete set of wheat–barley group-7 Robertsonian translocation chromosomes conferring an increased content of β-glucan

  • Tatiana V. Danilova
  • Bernd Friebe
  • Bikram S. Gill
  • Jesse Poland
  • Eric Jackson
Original Article

Abstract

Key message

A complete set of six compensating Robertsonian translocation chromosomes involving barley chromosome 7H and three chromosomes of hexaploid wheat was produced. Grain β-glucan content increased in lines containing 7HL.

Abstract

Many valuable genes for agronomic performance, disease resistance and increased yield have been transferred from relative species to wheat (Triticum aestivum L.) through whole-arm Robertsonian translocations (RobT). Although of a great value, the sets of available translocations from barley (Hordeum vulgare L.) are limited. Here, we present the production of a complete set of six compensating RobT chromosomes involving barley chromosome 7H and three group-7 chromosomes of wheat. The barley group-7 long-arm RobTs had a higher grain β-glucan content compared to the wheat control. The β-glucan levels varied depending on the temperature and were higher under hot conditions. Implicated in this increase, the barley cellulose synthase-like F6 gene (CslF6) responsible for β-glucan synthesis was physically mapped near the centromere in the long arm of barley chromosome 7H. Likewise, wheat CslF6 homoeologs were mapped near the centromere in the long arms of all group-7 wheat chromosomes. With the set of novel wheat–barley translocations, we demonstrate a valuable increase of β-glucan, along with a resource of genetic stocks that are likely to carry many other important genes from barley into wheat.

Notes

Acknowledgements

We thank W. John Raupp for critical editorial review of the manuscript and Duane Wilson for technical assistance; Paul St Amand for consultations on KASP; Rebecca Miller, for expertise and providing equipment for grain moisture measurement and milling; Alina Akhunova, for expertise and lab equipment for spectrophotometry and real time PCR; Anita Dille and Krishna S.V. Jagadish for providing growth chambers. This work was supported through sponsored research agreement between Kansas State University and General Mills Inc. EJ is an employee and stakeholder of General Mills Inc. This is Contribution number 18-078-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502, USA. Funding was provided by General Mills (US) (Grant nos. BG4412 and BG3641).

Compliance with ethical standards

Conflict of interest

The authors declare that they do not have conflict of interest.

Supplementary material

122_2017_3008_MOESM1_ESM.pdf (112 kb)
Supplementary material 1 (PDF 112 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences CenterKansas State UniversityManhattanUSA
  2. 2.General Mills NTS [AI]2 LabMinneapolisUSA

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