Molecular insights into the origin of the brown rust resistance gene Bru1 among Saccharum species
Analysis of 387 sugarcane clones using Bru 1 diagnostic markers revealed two possible sources of Bru 1 in Chinese cultivars: one from Saccharum spontaneum and another from Saccharum robustum of New Guinea.
Sugarcane brown rust (SBR) is an important fungal disease in many sugarcane production areas around the world, and can cause considerable yield losses in susceptible sugarcane cultivars. One major SBR resistance gene, named Bru1, initially identified from cultivar R570, was shown to be a major SBR resistance source in most of the sugarcane producing areas of the world. In this study, by using the two Bru1-associated markers, R12H16 and 9O20-F4, we surveyed the presence of Bru1 in a Chinese sugarcane germplasm collection of 387 clones, consisting of 228 hybrid cultivars bred by different Chinese sugarcane breeding establishments, 54 exotic hybrid cultivars introduced from other countries and 105 clones of sugarcane ancestral species. The Bru1-bearing haplotype was detected in 43.4% of Chinese sugarcane cultivars, 20.4% of exotic hybrid cultivars, and only 3.8% of ancestral species. Among the 33 Chinese cultivars for which phenotypes of resistance to SBR were available, Bru1 was present in 69.2% (18/26) of the resistant clones. Analyses of the allelic sequence variations of R12H16 and 9O20-F4 suggested two possible sources of Bru1 in Chinese cultivars: one from S. spontaneum and another from S. robustum of New Guinea. In addition, we developed an improved Bru1 diagnostic marker, 9O20-F4-HaeIII, which can eliminate all the false results of 9O20-F4-RsaI observed among S. spontaneum, as well as a new dominant Bru1 diagnostic marker, R12E03-2, from the BAC ShCIR12E03. Our results provide valuable information for further efforts of breeding SBR-resistant varieties, searching new SBR resistance sources and cloning of Bru1 in sugarcane.
This work was supported by a startup fund for distinguished scholars of Fujian Agriculture and Forestry University, and the Project of Education and Scientific Research of Young Teacher of Fujian (JA13102). The authors would like to thank Xin Lu for providing facilities and help in obtaining the leaf tissues from NNSGR.
Author contribution statement
YL conceived and designed the experiments; HW, PC and YY conducted the experiments; HW and YL processed the data; YL wrote the manuscript; AD reviewed the manuscript; all authors read and approved the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The authors declare that the experiments presented in this publication comply with the current laws of China and France.
Informed consent was obtained from all individual participants included in the study.
- Bremer G (1961b) Problems in breeding and cytology of sugarcane. II. The sugar cane breeding from a cytological view-point. Euphytica 10(2):121–258Google Scholar
- Canilha L, Chandel AK, Milessi TSS, Antunes FAF, Freitas WLC, Felipe MGA, Silva SS (2012) Bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification and ethanol fermentation. J Biomed Biotechnol 8:989572Google Scholar
- Costet L, Le Cunff L, Royaert S, Raboin LM, Hervouet C, Toubi L, Telismart H, Garsmeur O, Rousselle Y, Pauquet J, Nibouche S, Glaszmann JC, Hoarau JY, D’Hont A (2012) Haplotype structure around Bru1 reveals a narrow genetic basis for brown rust resistance in modern sugarcane cultivars. Theor Appl Genet 125(5):825–836CrossRefPubMedGoogle Scholar
- Daniels J, Daniels CA (1975) Geographical, historical and cultural aspects of the origin of the Indian and Chinese sugarcanes S. barberi and S. sinense. Sugarcane Breed Newslett 36:4–23Google Scholar
- Daniels J, Smith P, Paton N, Williams CA (1975) The origin of the genus Saccharum. Sugarcane Breed Newsett 36:24–39Google Scholar
- Dean JL, Tai PYP, Todd EH (1979) Sugarcane rust in Florida. Sugar J 42:10Google Scholar
- D’Hont A, Lu YH, Feldmann P, Glaszmann JC (1993) Cytoplasmic diversity in sugarcane revealed by heterologous probes. Sugar Cane 1993(1):12–15Google Scholar
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
- Glaszmann JC, Lu YH, Lanaud C (1990) Variation of nuclear ribosomal DNA in sugarcane. J Genet Breed 44:191–198Google Scholar
- Grivet L, Glaszmann JC, D’Hont A (2005) Molecular evidence of sugarcane evolution and domestication. In: Motley TJ, Zerega N, Cross H (eds) Darwin’s harvest: new approaches to the origins, evolution, and conservation of crops. Columbia University Press, New York, pp 49–66Google Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–99Google Scholar
- Le Cunff L, Garsmeur O, Raboin LM, Pauquet J, Telismart H, Selvi A, Grivet L, Philippe R, Begum D, Deu M, Costet L, Wing R, Glaszmann JC, D’Hont A (2008) Diploid/polyploid syntenic shuttle mapping and haplotype-specific chromosome walking toward a rust resistance gene (Bru1) in highly polyploid sugarcane (2n ~ 12x ~ 115). Genetics 180(1):649–660CrossRefPubMedPubMedCentralGoogle Scholar
- Liu YQ, Li YP, Liang WH, Song QD, Qin XL, Ye L (2015) Current situation of sugar cane industry in the world. World Agriculture 2015(8):147–152Google Scholar
- Luo J, Pan YB, Xu L, Zhang H, Yuan Z, Deng Z, Chen R, Que Y (2014) Cultivar evaluation and essential test locations identification for sugarcane breeding in China. Sci World J 2014:302753Google Scholar
- Panje RR (1972) The role of Saccharum spontaneum in sugarcane breeding. Proc Int Soc Sugar Cane Technol 14:217–223Google Scholar
- Peng SG (1980) The general situation of evolution of sugarcane varieties at home and abroad. J South Agric 10:12–15Google Scholar
- Peng SG (1996) Varietal evolution of sugarcane in Taiwan. Southwest China J Agric Sci 9(1):117–124Google Scholar
- Raboin LM, Oliveira KM, Lecunff L, Telismart H, Roques D, Butterfield M, Hoarau JY, D’Hont A (2006) Genetic mapping in sugarcane, a high polyploid, using bi-parental progeny: identification of a gene controlling stalk colour and a new rust resistance gene. Theor Appl Genet 112:1382–1391CrossRefPubMedGoogle Scholar
- Roach BT (1972) Nobilization of sugarcane. Proc Int Soc Sugar Cane Technol 14:206–216Google Scholar
- Roach BT (1977) Utilization of Saccharum spontaneum in sugarcane breeding. Proc Int Soc Sugar Cane Technol 16:43–57Google Scholar
- Ruan XY, Yan F, Sun CJ (1983) Occurrence of Puccinia erianthi on sugarcane in Yunnan province. Acta Mycologica Sinia 2:260–261Google Scholar
- Sreenivasan TV, Ahloowalia BS, Heinz DJ (1987) Cytogenetics. In: Heinz DJ (ed) Sugarcane improvement through breeding. Elsevier, Amsterdam, pp 143–210Google Scholar
- Wei Q, Yang BL, Gao ZJ (2015) Analysis of current situation of sugar cane industry. J Agric Mech Res 4:247–254Google Scholar
- Zhang XY (1996) Utilization of S. robustum descendants in sugarcane breeding. Sugarcane 3(1):14–18Google Scholar
- Zhang YX (2003) Sugarcane breeding strategies in Taiwan. Sugarcane Canesugar 2003(1):22–25Google Scholar
- Zhang Q, Qi YW, Zhang CM, Chen YS, Deng HH (2009) Pedigree analysis of genetic relationship among core parents of sugarcane in Mainland China. Guangdong Agric Sci 2009(10):44–48Google Scholar