Theoretical and Applied Genetics

, Volume 130, Issue 3, pp 495–504 | Cite as

Fine mapping of the chromosome 5B region carrying closely linked rust resistance genes Yr47 and Lr52 in wheat

  • Naeela Qureshi
  • Harbans Bariana
  • Kerrie Forrest
  • Matthew Hayden
  • Beat Keller
  • Thomas Wicker
  • Justin Faris
  • Elena Salina
  • Urmil Bansal
Original Article

Abstract

Key message

Fine mapping ofYr47andLr52in chromosome arm 5BS of wheat identified close linkage of the markersun180to both genes and its robustness for marker-assisted selection was demonstrated.

Abstract

The widely effective and genetically linked rust resistance genes Yr47 and Lr52 have previously been mapped in the short arm of chromosome 5B in two F3 populations (Aus28183/Aus27229 and Aus28187/Aus27229). The Aus28183/Aus27229 F3 population was advanced to generate an F6 recombinant inbred line (RIL) population to identify markers closely linked with Yr47 and Lr52. Diverse genomic resources including flow-sorted chromosome survey sequence contigs representing the orthologous region in Brachypodium distachyon, the physical map of chromosome arm 5BS, expressed sequence tags (ESTs) located in the 5BS6-0.81-1.00 deletion bin and resistance gene analog contigs of chromosome arm 5BS were used to develop markers to saturate the target region. Selective genotyping was also performed using the iSelect 90 K Infinium wheat SNP assay. A set of SSR, STS, gene-based and SNP markers were developed and genotyped on the Aus28183/Aus27229 RIL population. Yr47 and Lr52 are genetically distinct genes that mapped 0.4 cM apart in the RIL population. The SSR marker sun180 co-segregated with Lr52 and mapped 0.4 cM distal to Yr47. In a high resolution mapping population of 600 F2 genotypes Yr47 and Lr52 mapped 0.2 cM apart and marker sun180 was placed 0.4 cM distal to Lr52. The amplification of a different sun180 amplicon (195 bp) than that linked with Yr47 and Lr52 (200 bp) in 204 diverse wheat genotypes demonstrated its robustness for marker-assisted selection of these genes.

Notes

Acknowledgements

Naeela Qureshi acknowledges the University of Sydney for the USYDIS award. We thank the Grains Research and Development Corporation (GRDC) Australia for financial support through the Australian Cereal Rust Control Program.

Compliance with ethical standards

Conflict of interest

All authors have read the manuscript and declare that they have no conflict of interest.

Supplementary material

122_2016_2829_MOESM1_ESM.pdf (25 kb)
Supplementary material 1 (PDF 25 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Faculty of Agriculture, Food and Natural ResourcesThe University of Sydney Plant Breeding InstituteNarellanAustralia
  2. 2.Department of Economic Development, Jobs, Transport and ResourcesLa Trobe University AgriBioBundooraAustralia
  3. 3.Department of Plant and Microbial BiologyUniversity of ZurichZurichSwitzerland
  4. 4.USDA-ARS Cereal Crops Research UnitRed River Valley Agricultural Research CenterFargoUSA
  5. 5.Institute of Cytology and Genetics SB RASNovosibirskRussia

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