Abstract
Key message
We identified 15 potentially novel loci in addition to previously characterized leaf rust resistance genes from 1032 spring wheat accessions. Targeted AM subset panels were instrumental in revealing interesting loci.
Abstract
Leaf rust is a common disease of wheat, consistently reducing yields in many wheat-growing regions of the world. Although fungicides are commonly applied to wheat in the United States (US), genetic resistance can provide less expensive, yet effective control of the disease. Our objectives were to map leaf rust resistance genes in a large core collection of spring wheat accessions selected from the United States Department of Agriculture-Agricultural Research Service National Small Grains Collection (NSGC), determine whether previously characterized race-nonspecific resistance genes could be identified with our panel, and evaluate the use of targeted panels to identify seedling and adult plant resistance (APR) genes. Association mapping (AM) detected five potentially novel leaf rust resistance loci on chromosomes 2BL, 4AS, and 5DL at the seedling stage, and 2DL and 7AS that conditioned both seedling and adult plant resistance. In addition, ten potentially novel race-nonspecific resistance loci conditioned field resistance and lacked seedling resistance. Analyses of targeted subsets of the accessions identified additional loci not associated with resistance in the complete core panel. Using molecular markers, we also confirmed the presence and effectiveness of the race-nonspecific genes Lr34, Lr46, and Lr67 in our panel. Although most of the accessions in this study were susceptible to leaf rust in field and seedling tests, many resistance loci were identified with AM. Through the use of targeted subset panels, more loci were identified than in the larger core panels alone.
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Acknowledgements
We would like to acknowledge Amy Fox for organizing and curating all data collected, performing the seedling tests, and assisting with field evaluations. We would like to thank Kun Xiao for preparing the inoculum for seedling and greenhouse tests. We appreciate the hard work of Steve Haring, Nate Drews, and Arianh Smith for collecting data in the field. We are grateful to Liang Gao, Ahmed Sallam, and Liana Nice for providing expertise with analysis. Sheri Rynearson provided marker analysis on known resistance genes. We would like to thank the Triticeae Coordinated Agriculture Project (T-CAP) National Research Initiative Competitive Grant 2011-68002-30029 for providing funding for this project as well as training and an interactive, collaborative network throughout the project. All phenotypic data and genotypes are publicly available at the T3 database (http://triticeaetoolbox.org/) and the ARS-GRIN system (http://www.ars-grin.gov/).
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Turner, M.K., Kolmer, J.A., Pumphrey, M.O. et al. Association mapping of leaf rust resistance loci in a spring wheat core collection. Theor Appl Genet 130, 345–361 (2017). https://doi.org/10.1007/s00122-016-2815-y
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DOI: https://doi.org/10.1007/s00122-016-2815-y