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Fine mapping and candidate gene analysis of qHD5, a novel major QTL with pleiotropism for yield-related traits in rice (Oryza sativa L.)

Abstract

Key message

A major QTL for heading date, qHD5, was fine-mapped to a 52.59-kb region on the short arm of rice chromosome 5.

Abstract

Heading date (HD) is one of the most important traits that enables rice to adapt to seasonal differences and specific growth conditions in diverse growing regions. In this study, a major-effect quantitative trait locus (QTL), qHD5, was resolved as a single Medelian factor that causes NIL(BG1) and NIL(XLJ) (two near-isogenic lines (NILs) used in our study) to have at a minimum of 10-day difference in HD under both long-day and short-day conditions in rice. qHD5 was initially mapped to a 309.52-kb genomic region in our previous study. Here, using an advanced BC4F3 population and map-based cloning, we further narrowed the location of qHD5 to a 52.59-kb region between the H71 and RD502 markers. Sequence analysis revealed that Os05g03040, which putatively encodes an AP2 (APETALA2) transcription factor, has six single nucleotide polymorphisms (SNPs) between NIL(BG1) and NIL(XLJ). On this basis, this gene was concluded to be the most probable candidate gene for qHD5. Our results also showed that Hd3a, RFT1, Hd1, Ehd1, and Ghd7 were differentially expressed in the two NILs. Moreover, qHD5 was found to affect yield-related traits such as flag leaf width, flag leaf length, branch number, and 1000-grain weight.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (Grants 31101209, 31221004, and 31501290) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2013-CNRRI).

Author information

Correspondence to Li-Yong Cao or Shi-Hua Cheng.

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Conflict of interest

The authors declare no conflicts of interest regarding the publication of this paper.

Additional information

B. Sun and X.-D. Zhan contributed equally to this work.

The original version of this article was revised: A value (52.92-kb) under the heading ‘‘Key message’’ was incorrect. This error has been corrected.

An erratum to this article is available at http://dx.doi.org/10.1007/s00122-016-2821-0.

Communicated by M. Wissuwa.

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Sun, B., Zhan, X., Lin, Z. et al. Fine mapping and candidate gene analysis of qHD5, a novel major QTL with pleiotropism for yield-related traits in rice (Oryza sativa L.). Theor Appl Genet 130, 247–258 (2017). https://doi.org/10.1007/s00122-016-2787-y

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Keywords

  • Quantitative Trait Locus
  • Grain Yield
  • Major Quantitative Trait Locus
  • InDel Marker
  • Head Date