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Theoretical and Applied Genetics

, Volume 129, Issue 6, pp 1191–1201 | Cite as

FaRXf1: a locus conferring resistance to angular leaf spot caused by Xanthomonas fragariae in octoploid strawberry

  • Jack A. Roach
  • Sujeet Verma
  • Natalia A. Peres
  • Andrew R. Jamieson
  • W. Eric van de Weg
  • Marco C. A. M. Bink
  • Nahla V. Bassil
  • Seonghee Lee
  • Vance M. Whitaker
Original Article

Abstract

Key message

Angular leaf spot is a devastating bacterial disease of strawberry. Resistance from two wild accessions is highly heritable and controlled by a major locus on linkage group 6D.

Abstract

Angular leaf spot caused by Xanthomonas fragariae is the only major bacterial disease of cultivated strawberry (Fragaria ×ananassa). While this disease may cause reductions of up to 8 % of marketable yield in Florida winter annual production, no resistant cultivars have been commercialized. Wild accessions US4808 and US4809 were previously identified as resistant to the four genetic clades of X. fragariae, and introgression of the trait into commercial quality perennial-type germplasm was initiated. Previous reports indicated high heritability for the trait but proposed both single-locus and multi-locus inheritance models. The objective of this study was to determine the mode of inheritance of resistance, to identify causal loci, and to begin introgression of resistance into Florida-adapted germplasm. Resistance was observed in two years of field trials with inoculated plants that assayed four full-sib families descended from US4808 to US4809. Resistance segregated 1:1 in all families indicating control by a dominant allele at a single locus. Using a selective genotyping approach with the IStraw90 Axiom® SNP array and pedigree-based QTL detection, a single major-effect QTL was identified in two full-sib families, one descended from each resistant accession. High-resolution melt curve analysis validated the presence of the QTL in separate populations. The QTL was delimited to the 33.1–33.6 Mbp (F. vesca vesca v1.1 reference) and 34.8–35.3 Mbp (F. vesca bracteata v2.0 reference) regions of linkage group 6D for both resistance sources and was designated FaRXf1. Characterization of this locus will facilitate marker-assisted selection toward the development of resistant cultivars.

Keywords

Selective Genotyping Angular Leaf Spot Diseased Leaf Area Resistant Progeny Overhead Irrigation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors acknowledge Tomas Hasing, Dr. James Mertely, and Teresa Seijo for their assistance and guidance. This work was funded in part by the National Institute of Food and Agriculture, U.S. Department of Agriculture Specialty Crop Research Initiative project “RosBREED: Combining disease resistance with horticultural quality in new rosaceous cultivars” under award No 2014-51181-22378.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2016_2695_MOESM1_ESM.pdf (65 kb)
Supplementary material 1 (PDF 65 kb)
122_2016_2695_MOESM2_ESM.pdf (453 kb)
Supplementary material 2 (PDF 452 kb)
122_2016_2695_MOESM3_ESM.pdf (125 kb)
Supplementary material 3 (PDF 125 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Jack A. Roach
    • 1
  • Sujeet Verma
    • 1
  • Natalia A. Peres
    • 2
  • Andrew R. Jamieson
    • 3
  • W. Eric van de Weg
    • 4
  • Marco C. A. M. Bink
    • 5
  • Nahla V. Bassil
    • 6
  • Seonghee Lee
    • 1
  • Vance M. Whitaker
    • 1
  1. 1.Department of Horticultural Science, IFAS Gulf Coast Research and Education CenterUniversity of FloridaWimaumaUSA
  2. 2.Department of Plant Pathology, IFAS Gulf Coast Research and Education CenterUniversity of FloridaWimaumaUSA
  3. 3.Atlantic Food and Horticulture Research Centre, Agriculture and Agri-Food CanadaKentvilleCanada
  4. 4.Wageningen UR Plant BreedingWageningenThe Netherlands
  5. 5.Biometris, Wageningen University and Research CentreWageningenThe Netherlands
  6. 6.USDA-ARS, National Clonal Germplasm RepositoryCorvallisUSA

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