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Resynthesis of Brassica napus through hybridization between B. juncea and B. carinata


Key message

First report for the resynthesis of Brassica napus by recombining A and C genome from B. juncea and B. carinata , respectively. Also documents B genome introgressions in resynthesized B. napus.


Resynthesis of Brassica napus (AACC) was achieved by hybridizing Brassica juncea (AABB) with Brassica carinata (BBCC). This was facilitated by spontaneous chromosome doubling in the F1 hybrid (ABBC) to yield octaploid (AABBBBCC), elimination of extra B genome chromosomes in the resulting octaploid and in subsequent selfed generations, aided with directed selection for fertile plants having B. napus morphology. Twenty-five plants with varying degrees of resemblance to natural B. napus were identified from 17 A5 progenies and assayed for cytogenetic stability and genetic diversity. Majority of these plants, except six (2n = 38) were hyperploids (2n = 40–56). The six plants with 2n = 38 were designated as derived B. napus types. These showed an expected meiotic configuration of 19II at metaphase-I, with 19−19 distribution at anaphase-I. Genotyping based on A and C genome specific primers confirmed genetic identity of six derived (2n = 38) B. napus plants with natural types whereas genotyping with B genome specific primers indicated introgression of B genome segments. This was also confirmed by genomic in situ hybridization (GISH). Strong signals of B genome probe were detected, proving hitherto unreported genetic exchanges between B and A/C chromosomes. These introgressions possibly occurred en route five generations of selfing. Derived plants yielded fertile hybrids in crosses with natural B. napus var. GSC 6. The selfed derived plants as evaluated in A6 plant to progeny rows were morphologically similar to natural B. napus, and meiotically stable. Agronomic assessment of these progenies revealed variation for key morpho-physiological traits. Of special interest were the progenies with plants having oil content exceeding 47 % as against about 39–41 % in existing cultivars.

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We are grateful to Pat Heslop-Harrison and Trude Scharzacher for actually teaching us the technique of genomic in situ hybridization during their visit to our laboratory in 2012.

Author information

Correspondence to Surinder Singh Banga.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding information

Researches were carried out with the financial assistance from Grains Research & Development Corporation, Australia under the research project “Expanding the Brassica Germplasm Base through collaboration with China and India” awarded to Shashi Banga. SSB acknowledges salary support from Indian Council of Agricultural Research.

Additional information

Communicated by C. F. Quiros.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file 1 Meiotic configurations in hyperploid segregants: (a) 17II+1I, (b) 16II+3I, (c) 15II+5I, (d) 25II+5I, (e) 24II+4I,(f) early anaphase with 56 chromosomes, (g) 22II+2I, (h) 18II+4I. (i) 20II, (j) 19II+2I, (k) Pollen stainability of derivedB. napus (l) Pollen stainability of aberrant samples (TIFF 7217 kb)

Supplementary file 2 Variation for main fruting raceme in different derived B. napus genotypes and natural B. napus

Supplementary file 3 Graphical genotyping based on polymorphism for candidate gene based markers (TIFF 579 kb)

Supplementary file 4 Projected patterns of B genome chromatin substitutions in A genome chromosomes of derived B. napus (TIFF 1416 kb)

Supplementary file 5 Projected patterns of B genome chromatin substitutions in A genome chromosomes of derived B. napus (TIFF 1416 kb)

Supplementary file 6 Projected patterns of B genome chromatin substitutions in C genome chromosomes of derived B. napus (TIFF 1477 kb)

Supplementary file 7 Projected patterns of B genome chromatin substitutions in C genome chromosomes of derived B. napus (TIFF 701 kb)

Supplementary file 8 Dendrogramme showing relatedness among normal and derived B. napus genotypes (TIFF 1128 kb)

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Chatterjee, D., Banga, S., Gupta, M. et al. Resynthesis of Brassica napus through hybridization between B. juncea and B. carinata . Theor Appl Genet 129, 977–990 (2016). https://doi.org/10.1007/s00122-016-2677-3

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  • Ethylene Diamine Tetra Acetic Acid
  • Ethylene Diamine Tetra Acetic Acid
  • Genome Chromosome
  • Meiotic Metaphase
  • Segmental Introgression