Theoretical and Applied Genetics

, Volume 128, Issue 11, pp 2341–2350 | Cite as

Multiple loci not only Rf3 involved in the restoration ability of pollen fertility, anther exsertion and pollen shedding to S type cytoplasmic male sterile in maize

  • Yang Feng
  • Qi Zheng
  • Hui Song
  • Yi Wang
  • Hui Wang
  • Lijing Jiang
  • Jianbing Yan
  • Yonglian Zheng
  • Bing YueEmail author
Original Article


Key message

Thirty loci for fertility restoration of pollen fertility, anther exsertion and pollen shedding to maize CMS-S were identified by GWAS.


S type cytoplasmic male sterile (CMS-S) is the main type of CMS in maize; poor understanding of the genetic architecture of fertility restoration to CMS-S is one of the reasons to impede its utility in hybrid breeding. In this study, genome-wide identification of genetic loci for fertility restoration ability to CMS-S was firstly conducted with a set of testcrossing association mapping panel in three environments. A total of 19, 3 and 8 significant loci (P < 1.8 × 10−6, α = 1) for pollen fertility, anther exsertion and pollen shedding were identified, respectively, and individual locus explained up to 28.26 % of phenotypic variation. Of them, only Rf3, the main restorer-fertility gene of CMS-S, was identified for the three traits simultaneously. In addition, 83 candidate genes within the 100 kb extension regions of these loci were predicted. These results revealed that besides Rf3 multiple genetic loci and mechanisms are involved in the fertility restoration ability to CMS-S. Results in this study would provide important information for understanding the genetic architecture of fertility restoration to CMS-S in maize.


Cytoplasmic Male Sterility Favorable Allele Pollen Fertility Fertility Restoration Association Panel 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by a grant from the National Natural Science Foundation of China (31371635).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The authors declare that the experiments comply with the current laws of the country in which they were performed.

Supplementary material

122_2015_2589_MOESM1_ESM.pdf (99 kb)
Supplementary material 1 (PDF 99 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Yang Feng
    • 1
  • Qi Zheng
    • 1
  • Hui Song
    • 1
  • Yi Wang
    • 2
  • Hui Wang
    • 2
  • Lijing Jiang
    • 2
  • Jianbing Yan
    • 1
  • Yonglian Zheng
    • 1
  • Bing Yue
    • 1
    Email author
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina
  2. 2.Industrial Crops Research InstitutionHeilongjiang Academy of Land Reclamation of SciencesHarbinChina

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