Theoretical and Applied Genetics

, Volume 128, Issue 10, pp 2037–2046 | Cite as

Novel glucosinolate composition lacking 4-methylthio-3-butenyl glucosinolate in Japanese white radish (Raphanus sativus L.)

  • Masahiko Ishida
  • Tomohiro Kakizaki
  • Yasujiro Morimitsu
  • Takayoshi Ohara
  • Katsunori Hatakeyama
  • Hitoshi Yoshiaki
  • Junna Kohori
  • Takeshi NishioEmail author
Original Article


Key message

Genetic analysis and gene mapping of the 4-methylthio-3-butenyl glucosinolate-less trait of white radish were performed and a white radish cultivar with new glucosinolate composition was developed.


A spontaneous mutant having significantly low 4-methylthio-3-butenyl glucosinolate (4MTB-GSL) content was identified from a landrace of Japanese white radish (Raphanus sativus L.) through intensive evaluation of glucosinolate profiles of 632 lines including genetic resources and commercial cultivars using high-performance liquid chromatography (HPLC) analysis. A line lacking 4MTB-GSL was developed using the selected mutant as a gene source. Genetic analyses of F1, F2, and BC1F1 populations of this line suggested that the 4MTB-GSL-less trait is controlled by a single recessive allele. Using SNP and SCAR markers, 96 F2 plants were genotyped, and a linkage map having nine linkage groups with a total map distance of 808.3 cM was constructed. A gene responsible for the 4MTB-GSL-less trait was mapped between CL1753 and CL5895 at the end of linkage group 1. The genetic distance between these markers was 4.2 cM. By selfing and selection of plants lacking 4MTB-GSL, a new cultivar, ‘Daikon parental line No. 5', was successfully developed. This cultivar was characterized by glucoerucin, which accounted for more than 90 % of the total glucosinolates (GSLs). The total GSL content in roots was ca. 12 μmol/g DW, significantly lower than those in common white radish cultivars. Significance of this line in radish breeding is discussed.


Sinigrin Methanethiol BC1F1 Population White Radish Aliphatic GSLs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was partly supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN), Japan.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2015_2564_MOESM1_ESM.pdf (486 kb)
Supplementary Fig. 1. A linkage map of DNA markers for mapping of the 4MTB-GSL-less gene (4-MTB) (PDF 486 kb)
122_2015_2564_MOESM2_ESM.pdf (26 kb)
Supplementary Fig. 2. Segregation of glucoerucin content and 4MTB-GSL contents in F2 between ‘NMR154 N’ and ‘HAGHN’ (PDF 26 kb)
122_2015_2564_MOESM3_ESM.pdf (15.5 mb)
Supplementary Fig. 3. New white radish cultivar ‘Parental Line No. 5′ without 4MTB-GSL (PDF 15,864 kb)
122_2015_2564_MOESM4_ESM.pdf (94 kb)
Supplementary material 4 (PDF 94 kb)
122_2015_2564_MOESM5_ESM.pdf (9 kb)
Supplementary material 5 (PDF 8 kb)
122_2015_2564_MOESM6_ESM.pdf (51 kb)
Supplementary material 6 (PDF 50 kb)
122_2015_2564_MOESM7_ESM.pdf (55 kb)
Supplementary material 7 (PDF 55 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Masahiko Ishida
    • 1
  • Tomohiro Kakizaki
    • 2
  • Yasujiro Morimitsu
    • 3
  • Takayoshi Ohara
    • 2
  • Katsunori Hatakeyama
    • 2
  • Hitoshi Yoshiaki
    • 2
  • Junna Kohori
    • 2
  • Takeshi Nishio
    • 4
    Email author
  1. 1.NARO Institute of Vegetable and Tea Science, Tsukuba Vegetable Research StationTsukubaJapan
  2. 2.NARO Institute of Vegetable and Tea ScienceTsuJapan
  3. 3.The Department of Food and Nutritional Sciences, The Graduate School of Humanities and SciencesOchanomizu UniversityTokyoJapan
  4. 4.Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan

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