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Theoretical and Applied Genetics

, Volume 128, Issue 10, pp 2037–2046 | Cite as

Novel glucosinolate composition lacking 4-methylthio-3-butenyl glucosinolate in Japanese white radish (Raphanus sativus L.)

  • Masahiko Ishida
  • Tomohiro Kakizaki
  • Yasujiro Morimitsu
  • Takayoshi Ohara
  • Katsunori Hatakeyama
  • Hitoshi Yoshiaki
  • Junna Kohori
  • Takeshi NishioEmail author
Original Article

Abstract

Key message

Genetic analysis and gene mapping of the 4-methylthio-3-butenyl glucosinolate-less trait of white radish were performed and a white radish cultivar with new glucosinolate composition was developed.

Abstract

A spontaneous mutant having significantly low 4-methylthio-3-butenyl glucosinolate (4MTB-GSL) content was identified from a landrace of Japanese white radish (Raphanus sativus L.) through intensive evaluation of glucosinolate profiles of 632 lines including genetic resources and commercial cultivars using high-performance liquid chromatography (HPLC) analysis. A line lacking 4MTB-GSL was developed using the selected mutant as a gene source. Genetic analyses of F1, F2, and BC1F1 populations of this line suggested that the 4MTB-GSL-less trait is controlled by a single recessive allele. Using SNP and SCAR markers, 96 F2 plants were genotyped, and a linkage map having nine linkage groups with a total map distance of 808.3 cM was constructed. A gene responsible for the 4MTB-GSL-less trait was mapped between CL1753 and CL5895 at the end of linkage group 1. The genetic distance between these markers was 4.2 cM. By selfing and selection of plants lacking 4MTB-GSL, a new cultivar, ‘Daikon parental line No. 5', was successfully developed. This cultivar was characterized by glucoerucin, which accounted for more than 90 % of the total glucosinolates (GSLs). The total GSL content in roots was ca. 12 μmol/g DW, significantly lower than those in common white radish cultivars. Significance of this line in radish breeding is discussed.

Keywords

Sinigrin Methanethiol BC1F1 Population White Radish Aliphatic GSLs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was partly supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN), Japan.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2015_2564_MOESM1_ESM.pdf (486 kb)
Supplementary Fig. 1. A linkage map of DNA markers for mapping of the 4MTB-GSL-less gene (4-MTB) (PDF 486 kb)
122_2015_2564_MOESM2_ESM.pdf (26 kb)
Supplementary Fig. 2. Segregation of glucoerucin content and 4MTB-GSL contents in F2 between ‘NMR154 N’ and ‘HAGHN’ (PDF 26 kb)
122_2015_2564_MOESM3_ESM.pdf (15.5 mb)
Supplementary Fig. 3. New white radish cultivar ‘Parental Line No. 5′ without 4MTB-GSL (PDF 15,864 kb)
122_2015_2564_MOESM4_ESM.pdf (94 kb)
Supplementary material 4 (PDF 94 kb)
122_2015_2564_MOESM5_ESM.pdf (9 kb)
Supplementary material 5 (PDF 8 kb)
122_2015_2564_MOESM6_ESM.pdf (51 kb)
Supplementary material 6 (PDF 50 kb)
122_2015_2564_MOESM7_ESM.pdf (55 kb)
Supplementary material 7 (PDF 55 kb)

References

  1. Banga O (1976) Radish, Raphanus sativus (Cruciferae). In: Simmonds NW (ed) Evolution of Crop Plants. Longman, London, pp 60–62Google Scholar
  2. Bidart-Bouzat M, Kliebenstein DJ (2008) Differential levels of insect herbivory in the field associated with genotypic variation in glucosinolates in Arabidopsis thaliana. J Chem Ecol 34:1026–1037CrossRefPubMedGoogle Scholar
  3. Bjerg B, Sørensen H (1987) Quantitative analysis of glucosinolates and HPLC of intact glucosinolates. In: Wathelet J-P (ed) Glucosinolates in rapeseeds: Analytical aspects. Martinus Nijhoff Publishers, Dordrecht, pp 125–150CrossRefGoogle Scholar
  4. Bones AM, Rossiter JT (1996) The myrosinase-glucosinolate system, its organisation and biochemistry. Physiol Plantarum 97:194–208CrossRefGoogle Scholar
  5. Carlson DG, Axenbichler ME, van Etten CH (1985) Glucosinolate in radish cultivars. J Amer Soc Hort Sci 110:634–638Google Scholar
  6. Clarke DB (2010) Glucosinolates, structures and analysis in food. Analytical Methods 2:310–325CrossRefGoogle Scholar
  7. Cohen JH, Kristal AR, Stanford JL (2000) Fruit and vegetable intakes and prostate cancer risk. J Natl Cancer Inst 92:61–68CrossRefPubMedGoogle Scholar
  8. Ediage EN, Di Mavungu JD, Scippo ML, Schneider YJ, Larondelle Y, Callebaut A, Robbens J, Van Peteghem C, De Saeger S (2011) Screening, identification and quantification of glucosinolates in black radish (Raphanus sativus L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography-mass spectrometry. J Chromatogr A 1218:4395–4405CrossRefPubMedGoogle Scholar
  9. Fahey JW, Zalcmann AT, Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5–51CrossRefPubMedGoogle Scholar
  10. Fenwick GR, Heaney RK, Mullin WJ (1983) Glucosinolate and their breakdown products in food and plants. Crit Rev Food Sci Nutr 18:123–201CrossRefPubMedGoogle Scholar
  11. Friis P, Kjær A (1966) 4-Methylthio-3-butenyl isothiocyanate, the pungent principle of radish root. Acta Chem Scand 20:698–705CrossRefGoogle Scholar
  12. Gao M, Li G, McCombie W, Quiros C (2005) Comparative analysis of a transposon-rich Brassica oleracea BAC clone with its corresponding sequence in A. thaliana. Theor Appl Genet 111:949–955CrossRefPubMedGoogle Scholar
  13. Giamoustaris A, Mithen R (1996) Genetics of aliphatic glucosinolates. IV. Side-chain modification in Brassica oleracea. Theor Appl Genet 93:1006–1010CrossRefPubMedGoogle Scholar
  14. Halkier BA, Gershenzon J (2006) Biology and biochemistry of glucosinolates. Ann. Rev. Plant Biology 57:303–333CrossRefGoogle Scholar
  15. Herr I, Büchler MW (2010) Dietary constituents of broccoli and other cruciferous vegetables: implications for prevention and therapy of cancer. Cancer Treat Rev 36:377–383CrossRefPubMedGoogle Scholar
  16. Hirani AH, Li G, Zelmer CD, McVetty PBE, Asif M, Goyal A (2012) Molecular genetics of glucosinolate biosynthesis in Brassicas: Genetic manipulation and application aspects. In: Goyal A (ed) Crop Plant. doi:  10.5772/45646. Available from: http://www.intechopen.com/books/crop-plant/molecular-geneticsof-glucosinolate-biosynthesis-in-brassicas
  17. Ishida M, Morimitsu Y (2013) Chemical changes of the breakdown compounds of glucosinolate in processed food of the daikon without containing 4-methylthio-3-butenyl glucosinolate. J Japan Associ Odor Environ 44:307–314 (In Japanese) Google Scholar
  18. Ishida M, Takahata Y, Kaizuma N (2003) Simple and rapid method for the selection of individual rapeseed plants low in glucosinolates. Breed Sci 53:291–296CrossRefGoogle Scholar
  19. Ishida M, Kakizaki T, Ohara T, Morimitsu Y (2011) Development of a simple and rapid extraction method of glucosinolates from radish roots. Breed Sci 61:208–211CrossRefGoogle Scholar
  20. Ishida M, Nagata M, Ohara T, Kakizaki T, Hatakeyama K, Nishio T (2012) Small variation of glucosinolate composition in Japanese cultivars of radish (Raphanus sativus L.) requires simple quantitative analysis for breeding of glucosinolate component. Breed Sci 62:63–70PubMedCentralCrossRefPubMedGoogle Scholar
  21. Kitamura S (1958) Varieties and transitions of radish. In: Nishiyama I (ed) Japanese radish. Jpn Soc from Sci Tokyo, Japan, pp 1–19 (in Japanese) Google Scholar
  22. Kitashiba H, Li F, Hirakawa H, Kawanabe T, Zou Z, Hasegawa Y, Tonosaki K, Shirasawa S, Fukushima A, Yokoi S, Takahata Y, Kakizaki T, Ishida M, Okamoto S, Sakamoto K, Shirasawa K, Tabata S, Nishio T (2014) Draft Sequences of the radish (Raphanus sativus L.) Genome. DNA Res 21:481–490PubMedCentralCrossRefPubMedGoogle Scholar
  23. Kliebenstein DJ, Lambrix VM, Reichelt M, Gershenzon J, Mitchell-Olds T (2001) Gene duplication in the diversification of secondary metabolism: tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in Arabidopsis. Plant Cell 13:681–693PubMedCentralCrossRefPubMedGoogle Scholar
  24. Kroymann J, Textor S, Tokuhisa JG, Falk KL, Bartram S, Gershenzon J, Mitchell-Olds T (2001) A gene controlling variation in Arabidopsis glucosinolate composition is part of the methionine chain elongation pathway. Plant Phys 127:1077–1088CrossRefGoogle Scholar
  25. Li G, Quiros CF (2002) Genetic analysis, expression and molecular characterization of BoGSL-ELONG, a major gene involved in the aliphatic glucosinolate pathway of Brassica species. Genetics 162:1937–1943PubMedCentralPubMedGoogle Scholar
  26. Li G, Quiros CF (2003) In planta side-chain glucosinolate modification in Arabidopsis by introduction of dioxygenase Brassica homolog BoGSL-ALK. Theor Appl Genet 106:1116–1121PubMedGoogle Scholar
  27. Li G, Gao M, Yang B, Quiros CF (2003) Gene to gene alignment between the Brassica and Arabidopsis genomes by transcriptional mapping. Theor Appl Genet 107:168–180CrossRefPubMedGoogle Scholar
  28. Li F, Hasegawa Y, Saito M, Shirasawa S, Fukushima A, Ito T, Fujii H, Kishitani S, Kitashiba H, Nishio T (2011) Extensive chromosome homoeology among Brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.). DNA Res 18:401–411PubMedCentralCrossRefPubMedGoogle Scholar
  29. Liu et al (2014) The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes. Nat Commun 5:3930PubMedCentralPubMedGoogle Scholar
  30. Melchini A, Traka MH (2010) Biological profile of erucin: a new promising anticancer agent from cruciferous vegetables. Toxins 2:593–612PubMedCentralCrossRefPubMedGoogle Scholar
  31. Millan S, Sampedro MC, Gallejones P, Castellon A, Ibargoitia ML, Goicolea MA, Barrio RJ (2009) Identification and quantification of glucosinolates in rapeseed using liquid chromatography-ion trap mass spectrometry. Anal Bioanal Chem 394:1661–1669CrossRefPubMedGoogle Scholar
  32. Mithen RF, Clarke J, Lister C, Dean C (1995) Genetics of aliphatic glucosinolates. III. Side chain structure of aliphatic glucosinolates in Arabidopsis thaliana. Heredity 74:210–215CrossRefGoogle Scholar
  33. Mithen RF, Dekker M, Verkerk R, Rabot S, Johnson LT (2000) The nutritional significance, biosynthesis and bioavailability of glucosinolates in human foods. J Sci Food Agric 80:967–984CrossRefGoogle Scholar
  34. Montaut S, Barillari J, Iori R, Rollin P (2010) Glucoraphasatin: chemistry, occurrence, and biological properties. Phytochemistry 71:6–12CrossRefPubMedGoogle Scholar
  35. Moon JK, Kim JR, Ahn YJ, Shibamoto T (2010) Analysis and anti-Helicobacter activity of sulforaphane and related compounds present in broccoli (Brassica oleracea L.) sprouts. J Agric Food Chem 58:6672–6677CrossRefPubMedGoogle Scholar
  36. Nishio T, Kusaba M, Watanabe M, Hinata K (1996) Registration of S alleles in Brassica campestris L by the restriction fragment sizes of SLGs. Theor Appl Genet 92:388–394CrossRefPubMedGoogle Scholar
  37. Ozawa Y, Kawakishi S, Uda Y, Maeda Y (1990a) Isolation and identification of a novel b-carboline derivative in salted radish roots, Raphanus sativus L. Agr Biol Chem 54:1241–1245CrossRefGoogle Scholar
  38. Ozawa Y, Uda Y, Kawakishi S (1990b) Generation of b-carboline derivative, the yellowish precursor of processed radish roots, from 4-methylthio-3-butenyl isothiocyanate and L-tryptophan. Agr Biol Chem 54:1849–1851CrossRefGoogle Scholar
  39. Pedras MS, Chumala PB, Suchy M (2003) Phytoalexins from Thlaspi arvense, a wild crucifer resistant to virulent Leptosphaeria maculans: structures, syntheses and antifungal activity. Phytochemistry 64:949–956CrossRefPubMedGoogle Scholar
  40. Rosa EAS, Heaney RK, Fenwick GR, Portas CAM (1997) Glucosinolates in crop plants. Hortic Rev 19:99–125Google Scholar
  41. Shiokai S, Kitashiba H, Nishio T (2010a) Prediction of the optimum hybridization conditions of dot-blot-SNP analysis using estimated melting temperature of oligonucleotide probes. Plant Cell Rep 29:829–834CrossRefPubMedGoogle Scholar
  42. Shiokai S, Shirasawa K, Sato Y, Nishio T (2010b) Improvement of the dot-blot-SNP technique for efficient and cost-effective genotyping. Mol Breed 25:179–185CrossRefGoogle Scholar
  43. Shirasawa K, Shiokai S, Yamaguchi M, Kishitani S, Nishio T (2006) Dot-blot-SNP analysis for practical plant breeding and cultivar identification in rice. Theor Appl Genet 113:147–155CrossRefPubMedGoogle Scholar
  44. Sønderby IE, Geu-Flores F, Halkier BA (2010) Biosynthesis of glucosinolates-gene discovery and beyond. Trends Plant Sci 15:283–290CrossRefPubMedGoogle Scholar
  45. Takahashi A, Yamada T, Uchiyama Y, Hayashi S, Kumakura K, Takahashi H, Kimura H, Matsuoka H (2015) Generation of the antioxidant yellow pigment derived from 4-methylthio-3-butenyl isothiocyanate in salted radish roots (takuan-zuke). Biosci Biotech Biochem. doi: 10.1080/09168451.2015.1032881 Google Scholar
  46. Uda Y, Matsuoka H, Kumagai H, Maeda Y (1993) Stability and antimicrobial property of 4-methylthio-3-butenyl isothiocyanate, the pungent principle in radish. Nippon Shokuhin Kogyo Gakkaishi 40:743–746CrossRefGoogle Scholar
  47. Wang H, Wu J, Sun S, Liu B, Cheng F, Sun R, Wang X (2011) Glucosinolate biosynthetic genes in Brassica rapa. Gene 487:135–142CrossRefPubMedGoogle Scholar
  48. Wang Y, Pan Y, Liu Z, Zhu X, Zhai L, Xu L, Yu R, Gong Y, Liu L (2013) De novo transcriptome sequencing of radish (Raphanus sativus L.) and analysis of major genes involved in glucosinolate metabolism. BMC Genom 14:836CrossRefGoogle Scholar
  49. Zasada IA, Ferris H (2004) Nematode suppression with brassicaceous amendments: application based upon glucosinolate profiles. Soil Biology and Biochemistry 36:1017–1024CrossRefGoogle Scholar
  50. Zou Z, Ishida M, Li F, Kakizaki T, Suzuki S, Kitashiba H, Nishio T (2013) QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L. PLoS One 8:e53541PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Masahiko Ishida
    • 1
  • Tomohiro Kakizaki
    • 2
  • Yasujiro Morimitsu
    • 3
  • Takayoshi Ohara
    • 2
  • Katsunori Hatakeyama
    • 2
  • Hitoshi Yoshiaki
    • 2
  • Junna Kohori
    • 2
  • Takeshi Nishio
    • 4
    Email author
  1. 1.NARO Institute of Vegetable and Tea Science, Tsukuba Vegetable Research StationTsukubaJapan
  2. 2.NARO Institute of Vegetable and Tea ScienceTsuJapan
  3. 3.The Department of Food and Nutritional Sciences, The Graduate School of Humanities and SciencesOchanomizu UniversityTokyoJapan
  4. 4.Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan

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