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Theoretical and Applied Genetics

, Volume 128, Issue 10, pp 2019–2035 | Cite as

Wide-genome QTL mapping of fruit quality traits in a tomato RIL population derived from the wild-relative species Solanum pimpinellifolium L.

  • Carmen Capel
  • Asunción Fernández del Carmen
  • Juan Manuel Alba
  • Viviana Lima-Silva
  • Francesc Hernández-Gras
  • María Salinas
  • Albert Boronat
  • Trinidad Angosto
  • Miguel A. Botella
  • Rafael Fernández-Muñoz
  • Antonio Granell
  • Juan Capel
  • Rafael Lozano
Original Article

Abstract

Key message

QTL and candidate genes associated to fruit quality traits have been identified in a tomato genetic map derived from Solanum pimpinellifolium L., providing molecular tools for marker-assisted breeding.

Abstract

The study of genetic, physiological, and molecular pathways involved in fruit development and ripening has considered tomato as the model fleshy-fruited species par excellence. Fruit quality traits regarding organoleptic and nutritional properties are major goals for tomato breeding programs since they largely decide the acceptance of tomato in both fresh and processing markets. Here we report the genetic mapping of single-locus and epistatic quantitative trait loci (QTL) associated to the fruit size and content of sugars, acids, vitamins, and carotenoids from the characterization of a RIL population derived from the wild-relative Solanum pimpinellifolium TO-937. A genetic map composed of 353 molecular markers including 13 genes regulating fruit and developmental traits was generated, which spanned 1007 cM with an average distance between markers of 2.8 cM. Genetic analyses indicated that fruit quality traits analyzed in this work exhibited transgressive segregation and that additive and epistatic effects are the major genetic basis of fruit quality traits. Moreover, most mapped QTL showed environment interaction effects. FrW7.1 fruit size QTL co-localized with QTL involved in soluble solid, vitamin C, and glucose contents, dry weight/fresh weight, and most importantly with the Sucrose Phosphate Synthase gene, suggesting that polymorphisms in this gene could influence genetic variation in several fruit quality traits. In addition, 1-deoxy-D-xylulose 5-phosphate synthase and Tocopherol cyclase genes were identified as candidate genes underlying QTL variation in beta-carotene and vitamin C. Together, our results provide useful genetic and molecular information regarding fruit quality and new chances for tomato breeding by implementing marker-assisted selection.

Keywords

Quantitative Trait Locus Lycopene Recombinant Inbred Line Quantitative Trait Locus Analysis Titratable Acidity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Thanks are due to Dr. Fernando Yuste-Lisbona and Dr. Antonio Monforte for critical review of the manuscript. This work was funded by the ESPSOL project from the Fundación Genoma of the Spanish Ministerio de Ciencia y Tecnología. We also thank research facilities provided by the Campus de Excelencia Internacional Agroalimentario (CeiA3).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2015_2563_MOESM1_ESM.pdf (118 kb)
Supplementary Fig. 1 Blox plot representation of the analyzed fruit quality traits (Fruit weight; Titriable acidity; pH; Citric acid; Malic acid; Total soluble solid; Glucose; Fructose; Fructose/Glucose; Beta-carotene; Lycopene). Center lines show the average values and box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; outliers are represented by dots. Depending on the trait considered, the number of sample points ranged from 163 to 169 in the first growing cycle, and it was 169 in the second growing cycle (PDF 117 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Carmen Capel
    • 1
  • Asunción Fernández del Carmen
    • 2
  • Juan Manuel Alba
    • 3
  • Viviana Lima-Silva
    • 3
  • Francesc Hernández-Gras
    • 4
  • María Salinas
    • 1
  • Albert Boronat
    • 4
  • Trinidad Angosto
    • 1
  • Miguel A. Botella
    • 3
  • Rafael Fernández-Muñoz
    • 3
  • Antonio Granell
    • 2
  • Juan Capel
    • 1
  • Rafael Lozano
    • 1
  1. 1.Departamento de Biología y Geología (Genética), Edificio CITE II-B, Centro de Investigación en Biotecnología Agroalimentaria (BITAL)Universidad de AlmeríaAlmeríaSpain
  2. 2.Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC)Universidad Politécnica de ValenciaValenciaSpain
  3. 3.Instituto de Hortofruticultura Subtropical y Mediterránea La MayoraUniversidad de Málaga-Consejo Superior de Investigaciones CientíficasAlgarrobo-CostaSpain
  4. 4.Departamento de Bioquímica y Biología Molecular, Facultad de BiologíaUniversidad de BarcelonaBarcelonaSpain

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