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Theoretical and Applied Genetics

, Volume 128, Issue 9, pp 1827–1837 | Cite as

Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length

  • Jing Zhang
  • Jinpeng Zhang
  • Weihua Liu
  • Haiming Han
  • Yuqing Lu
  • Xinming Yang
  • Xiuquan Li
  • Lihui LiEmail author
Original Paper

Abstract

Key message

This study explored the genetic constitutions of wheat-Agropyron cristatum 6P chromosomal translocation and determined the effects of 6P intercalary chromosome segment on thousand-grain weight and spike length in wheat.

Abstract

Crop wild relatives provide rich genetic resources for wheat improvement. Introduction of alien genes from Agropyron cristatum into common wheat can broaden its genetic diversity. In this study, radiation-induced wheat-A. cristatum translocation line Pubing3035 derived from the offspring of wheat-A. cristatum 6P chromosomes addition line was identified and analyzed using genomic in situ hybridization (GISH), dual-color fluorescence in situ hybridization (FISH), and molecular markers. GISH analysis revealed that Pubing3035 was a Ti1AS-6PL-1AS·1AL intercalary translocation. The breakpoint was pinpointed to locate near the centromeric region on the short arm of wheat chromosome 1A based on a constructed F2 linkage map and it was flanked by markers SSR12 and SSR263. The genotypic data, combined with the phenotypes, indicated that A. cristatum 6P chromosomal segment played an important role in regulating the thousand-grain weight and spike length. On average, the thousand-grain weight and spike length in translocation individuals were approximately 2.5 g higher and 0.7 cm longer than those in non-translocation individuals in F2 and BC1F1 populations. The clusters of quantitative trait loci for thousand-grain weight, spike length, and spikelet density contributed by 6P chromosome segment were mapped between A. cristatum unique marker Agc7155 and wheat marker SSR263, which, respectively, explained phenotypic variance of 24.96, 12.38 and 17.20 % with an LOD of 10.63, 4.89 and 5.59. Overall, the translocation Pubing3035 had a positive effect on the yield of wheat, which laid the foundation for the localization of A. cristatum excellent genes and made itself a promising and valuable germplasm for wheat improvement.

Keywords

Quantitative Trait Locus Common Wheat Translocation Line BC1F1 Population Spike Length 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the Grants from the National Basic Research Program of China (973 Grant No. 2011CB100104), the National High Technology Research and Development Program of China (863 Grant no. 2011AA100101), the National Natural Science Foundation of China (Grant No. 31071416).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2015_2550_MOESM1_ESM.xlsx (11 kb)
Supplementary material 1 (XLSX 11 kb)
122_2015_2550_MOESM2_ESM.xlsx (20 kb)
Supplementary material 2 (XLSX 19 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Jing Zhang
    • 1
  • Jinpeng Zhang
    • 1
  • Weihua Liu
    • 1
  • Haiming Han
    • 1
  • Yuqing Lu
    • 1
  • Xinming Yang
    • 1
  • Xiuquan Li
    • 1
  • Lihui Li
    • 1
    Email author
  1. 1.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina

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