Theoretical and Applied Genetics

, Volume 128, Issue 7, pp 1421–1430 | Cite as

Marker-assisted breeding of a LOX-3-null rice line with improved storability and resistance to preharvest sprouting

  • Yasuhiro SuzukiEmail author
  • Kiyoyuki Miura
  • Akiko Shigemune
  • Hideki Sasahara
  • Hisatoshi Ohta
  • Yasuki Uehara
  • Tetsuya Ishikawa
  • Shigeki Hamada
  • Kenta Shirasawa
Original Paper


Key message

Breakage of the tight linkage between rice seed lipoxygenase - 3 and easy preharvest sprouting trait led to breeding of lines with few stale flavors after long storage and desirable preharvest sprouting resistance.


Lipoxygenase-3 (LOX-3) is involved in the production of volatile constituents in stored rice, and the development of stale flavor is delayed in LOX-3 null rice. In the process of breeding new LOX-3-null lines with long storability, we found a close association between LOX-3 and preharvest sprouting resistance. To determine whether this relationship was due to the tight linkage of two genes or the pleiotropic effect of LOX-3, we performed marker-assisted selection using a BC3F3 population derived from crosses between LOX-3-present/preharvest sprouting-resistant lines and LOX-3-null/preharvest susceptible lines. In one individual, a recombination event occurred 13 kb downstream of LOX-3 (RM15750) and a significant quantitative trait locus, namely qPHS3, for easy preharvest sprouting trait (LOD = 10.4) was detected in an 842-kb region between RM15711 and RM15768. Using BC3F4 and BC3F5 populations, we succeeded in selecting LOX-3-absent and preharvest sprouting-resistant lines with only a 393-kb introgressed chromosome segment from the donor line for LOX-3-null at the LOX-3 locus on chromosome 3. This result indicated that the LOX-3 gene and the locus affecting preharvest sprouting are distinct. The selected line was named ‘Hokuriku 244’. Sensory testing of rice grains with and without LOX-3 confirmed that stale flavor production in LOX-3-null rice during storage was lower than in normal LOX-3 rice. These results indicated that rice varieties with little stale flavor after long storage and preharvest sprouting resistance had been selected.


Simple Sequence Repeat Marker Rice Variety Seed Dormancy Brown Rice Composite Interval Mapping 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Ms. Chika Hashimoto and Sumiko Sakurai for technical assistance, Dr. Tsuyu Ando of the STAFF Institute for genotyping and Dr. Ikuo Ashikawa (NARO Inst. Crop Sci.) for helpful discussions of an early version of this manuscript. This work was supported in part by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (the research project; Development of New rice Varieties using Genomic Breeding Technology). We would like to thank Enago ( for the English language review.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2015_2516_MOESM1_ESM.pptx (1.6 mb)
Supplementary material 1 (PPTX 158 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Yasuhiro Suzuki
    • 1
    Email author
  • Kiyoyuki Miura
    • 1
    • 2
  • Akiko Shigemune
    • 2
    • 3
  • Hideki Sasahara
    • 2
  • Hisatoshi Ohta
    • 2
    • 4
  • Yasuki Uehara
    • 2
    • 5
  • Tetsuya Ishikawa
    • 1
    • 4
  • Shigeki Hamada
    • 1
    • 6
  • Kenta Shirasawa
    • 1
    • 7
  1. 1.NAROInstitute of Crop ScienceTsukubaJapan
  2. 2.NAROAgric. Res. Cent., Hokuriku Res. Cent.JoetsuJapan
  3. 3.NAROKinki-Chuugoku-Shikoku Agric. Res. Cent.FukuyamaJapan
  4. 4.NAROTohoku Agric. Res. Cent.MoriokaJapan
  5. 5.Dainihon-noukaiMinato-kuJapan
  6. 6.Fac. Agric. and Life Sci.Hirosaki Univ.HirosakiJapan
  7. 7.Kazusa DNA Res. Inst.ChibaJapan

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