Theoretical and Applied Genetics

, Volume 128, Issue 7, pp 1307–1318 | Cite as

Multi-location wheat stripe rust QTL analysis: genetic background and epistatic interactions

  • M. Dolores Vazquez
  • Robert Zemetra
  • C. James Peterson
  • Xianming M. Chen
  • Adam Heesacker
  • Christopher C. Mundt
Original Paper


Key message

Epistasis and genetic background were important influences on expression of stripe rust resistance in two wheat RIL populations, one with resistance conditioned by two major genes and the other conditioned by several minor QTL.


Stripe rust is a foliar disease of wheat (Triticum aestivum L.) caused by the air-borne fungus Puccinia striiformis f. sp. tritici and is present in most regions around the world where commercial wheat is grown. Breeding for durable resistance to stripe rust continues to be a priority, but also is a challenge due to the complexity of interactions among resistance genes and to the wide diversity and continuous evolution of the pathogen races. The goal of this study was to detect chromosomal regions for resistance to stripe rust in two winter wheat populations, ‘Tubbs’/‘NSA-98-0995’ (T/N) and ‘Einstein’/‘Tubbs’ (E/T), evaluated across seven environments and mapped with diversity array technology and simple sequence repeat markers covering polymorphic regions of ≈1480 and 1117 cM, respectively. Analysis of variance for phenotypic data revealed significant (P < 0.01) genotypic differentiation for stripe rust among the recombinant inbred lines. Results for quantitative trait loci/locus (QTL) analysis in the E/T population indicated that two major QTL located in chromosomes 2AS and 6AL, with epistatic interaction between them, were responsible for the main phenotypic response. For the T/N population, eight QTL were identified, with those in chromosomes 2AL and 2BL accounting for the largest percentage of the phenotypic variance.


Stripe Rust Epistatic Interaction Adult Plant Resistance Quantitative Trait Locus Stripe Rust Resistance 



We thank the Oregon Wheat Commission and Warren Kronstad Endowment for funding the project. We thank the Columbia Basin Agricultural Research Center staff, Kathryn Sackett, Larae Wallace, Mark Larson, and Paul Severns for their excellent technical assistance.

Conflict of interest

The authors declare that they have no conflict of interests.

Ethical standards

The experiments comply with the current US laws.

Supplementary material

122_2015_2507_MOESM1_ESM.pdf (48 kb)
Supplementary Fig. 1 Wheat cross Tubbs/NSA-98-0995 (T/N) population linkage maps (PDF 48 kb)
122_2015_2507_MOESM2_ESM.pdf (43 kb)
Supplementary Fig. 2 Wheat cross Einstein/Tubbs (E/T) population linkage maps (PDF 43 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • M. Dolores Vazquez
    • 1
  • Robert Zemetra
    • 2
  • C. James Peterson
    • 3
  • Xianming M. Chen
    • 4
  • Adam Heesacker
    • 2
  • Christopher C. Mundt
    • 1
  1. 1.Department of Botany and Plant PathologyOregon State UniversityCorvallisUSA
  2. 2.Department of Crop and Soil ScienceOregon State UniversityCorvallisUSA
  3. 3.Limagrain Cereals SeedsFort CollinsUSA
  4. 4.US Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology, and Disease Research Unit, Department of Plant PathologyWashington State UniversityPullmanUSA

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