Theoretical and Applied Genetics

, Volume 128, Issue 2, pp 273–282 | Cite as

Variation in genome composition of blue-aleurone wheat

  • Veronika Burešová
  • David Kopecký
  • Jan Bartoš
  • Petr Martinek
  • Nobuyoshi Watanabe
  • Tomáš Vyhnánek
  • Jaroslav Doležel
Original Paper

Abstract

Key message

Different blue-aleurone wheats display major differences in chromosome composition, ranging from disomic chromosome additions, substitutions, single chromosome arm introgressions and chromosome translocation ofThinopyrum ponticum.

Abstract

Anthocyanins are of great importance for human health due to their antioxidant, anti-inflammatory, anti-microbial and anti-cancerogenic potential. In common wheat (Triticum aestivum L.) their content is low. However, elite lines with blue aleurone exhibit significantly increased levels of anthocyanins. These lines carry introgressed chromatin from wild relatives of wheat such as Thinopyrum ponticum and Triticum monococcum. The aim of our study was to characterize genomic constitutions of wheat lines with blue aleurone using genomic and fluorescence in situ hybridization. We used total genomic DNA of Th. ponticum and two repetitive DNA sequences (GAA repeat and the Afa family) as probes to identify individual chromosomes. This enabled precise localization of introgressed Th. ponticum chromatin. Our results revealed large variation in chromosome constitutions of the blue-aleurone wheats. Of 26 analyzed lines, 17 carried an introgression from Th. ponticum; the remaining nine lines presumably carry T. monococcum chromatin undetectable by the methods employed. Of the Th. ponticum introgressions, six different types were present, ranging from a ditelosomic addition (cv. Blue Norco) to a disomic substitution (cv. Blue Baart), substitution of complete (homologous) chromosome arms (line UC66049) and various translocations of distal parts of a chromosome arm(s). Different types of introgressions present support a hypothesis that the introgressions activate the blue aleurone trait present, but inactivated, in common wheat germplasm.

References

  1. Abdel-Aal ESM, Young JC, Rabalski I (2006) Anthocyanin composition in black, blue, pink, purple, and red cereal grains. J Agric Food Chem 54:4696–4704CrossRefGoogle Scholar
  2. Abrouk M, Šimková H, Pingault L, Martis M, Jarve K, Paux E, International Wheat Genome Sequencing Consortium, Dolezel J, Valarik M (2014) In silico identification and characterization of wheat 4AL—T. militinae introgression. Plant and Animal Genome XXII Web. https://www.pag.confex.com/pag/xxii/webprogram/Paper10789.html. Accessed 11 August 2014
  3. Afaq F, Syed DN, Malik A, Hadi N, Sarfaraz S, Kweon MH, Khan N, Zaid MA, Mukhtar H (2007) Delphinidin, an anthocyaindin in pigmented fruits and vegetables, protects human HaCaT keratinocytes and mouse skin against UVB-mediated oxidative stress and apoptosis. J Invest Dermatol 127:222–232PubMedCrossRefGoogle Scholar
  4. Anderson JW, Hanna TJ, Peng X, Kryscio R (2000) Whole grain foods and heart disease risk. J Am Coll Nutr 19:291–299CrossRefGoogle Scholar
  5. Arbuzova VS, Badaeva ED, Efremova TT, Osadchaya TS, Trubacheeva NV, Dobrovolskaya OB (2012) Cytogenetic study of the blue grain line of the common wheat cultivar Saratovskaya 29. Russ J Genet 48:785–791CrossRefGoogle Scholar
  6. Bowen-Forbes CS, Zhang Y, Nair MG (2010) Anthocyanin content, antioxidant, anti-inflammatory and anticancer properties of blackberry and raspberry fruits. J Food Compos Anal 23:554–560CrossRefGoogle Scholar
  7. Cermeno MC, Zeller FJ (1986) Identity and cytological characterisation of alien chromosomes conferring blue aleurone colour in common wheat. 1st International. symp chromosome engineering in plants. Xian, China, pp 123–125Google Scholar
  8. Clifford MN (2000) Anthocyanins—nature, occurrence and dietary burden. J Sci Food Agric 80:1063–1072CrossRefGoogle Scholar
  9. Dubcovsky J, Luo MC, Zhong GY, Bransteitter R, Desai A, Kilian A, Kleinhofs A, Dvorak J (1996) Genetic map of diploid wheat, Triticum monococcum L, and its comparison with maps of Hordeum vulgare L. Genetics 143:983–999PubMedCentralPubMedGoogle Scholar
  10. Escribano-Bailon MT, Santos-Buelga C, Rivas-Gonzalo JC (2004) Anthocyanins in cereals. J Chromatogr A 1054:129–141PubMedCrossRefGoogle Scholar
  11. Ficco DBM, Mastrangelo AM, Trono D, Borrelli GM, De Vita P, Fares C, Beleggia R, Platani C, Papa R (2014) The colours of durum wheat: a review. Crop Pasture Sci 65:1–15Google Scholar
  12. Gao L, Mazza G (1994) Quantitation and distribution of simple and acylated anthocyanins and other phenolics in blueberries. J Food Sci 59:1057–1059CrossRefGoogle Scholar
  13. Gao JW, Liu JZ, Li B, Feng BS, Yu GQ, Li ZS (2000) Preliminary study on pigments in seed aleurone layer of blue-grained wheat. Acta Botanica Boreali-Occidentalia Sinica 20:936–941Google Scholar
  14. Giorgi D, Farina A, Grosso V, Gennaro A, Ceoloni C, Lucretti S (2013) FISHIS: fluorescence in situ hybridization in suspension and chromosome flow sorting made easy. PLOS ONE 8:e57994PubMedCentralPubMedCrossRefGoogle Scholar
  15. Harborne JB, Williams CA (2001) Anthocyanins and other flavonoids. Nat Prod Rep 18:310–333PubMedCrossRefGoogle Scholar
  16. Himi E, Noda K (2004) Isolation and location of three homoeologous dihydroflavonol-4-reductase (DFR) genes of wheat and their tissue-dependent expression. J Exp Bot 55:365–375PubMedCrossRefGoogle Scholar
  17. Horbowicz M, Kosson R, Grzesiuk A, Debski H (2008) Anthocyanins of fruits and vegetables—their occurrence, analysis and role in human nutrition. Veg Crop Res Bull 68:5–22Google Scholar
  18. Hou D-X, Fujii M, Terahara N, Yoshimoto M (2004) Molecular mechanisms behind the chemopreventive effects of anthocyanidins. J Biomed Biotechnol 2004:321–325PubMedCentralPubMedCrossRefGoogle Scholar
  19. Jan CC, Dvorak J, Qualset CO, Soliman KM (1981) Selection and identification of a spontaneous alien chromosome translocation in wheat. Genetics 98:389–398PubMedCentralPubMedGoogle Scholar
  20. Katsube N, Iwashita K, Tsushida T, Yamaki K, Kobori M (2003) Induction of apoptosis in cancer cells by bilberry (Vaccinium myrtillus) and the anthocyanins. J Agric Food Chem 51:68–75PubMedCrossRefGoogle Scholar
  21. Keppenne VD, Baenziger PS (1990) Inheritance of the blue aleurone trait in diverse wheat crosses. Genome 33:525–529CrossRefGoogle Scholar
  22. Knievel DC, Abdel-Aal E-SM, Rabalski I, Nakamura T, Hucl P (2009) Grain color development and the inheritance of high anthocyanin blue aleurone and purple pericarp in spring wheat (Triticum aestivum L.). J Cereal Sci 50:113–120CrossRefGoogle Scholar
  23. Knott DR (1958) The inheritance in wheat of a blue endosperm color derived from Agropyron elongatum. Can J Bot 36:571–574CrossRefGoogle Scholar
  24. Kubaláková M, Kovářová P, Suchánková P, Číhalíková J, Bartoš J, Lucretti S, Watanabe N, Kianian SF, Doležel J (2005) Chromosome sorting in tetraploid wheat and its potential for genome analysis. Genetics 170:823–829PubMedCentralPubMedCrossRefGoogle Scholar
  25. Lamy S, Blanchette M, Michaud-Levesque J, Lafleur R, Durocher Y, Moghrabi A, Barrette S, Gingras D, Beliveau R (2006) Delphidin, a dietary anthocyanidin, inhibits vascular endothelial growth factor receptor-2 phosphorylation. Cancerogenesis 27:989–996CrossRefGoogle Scholar
  26. Lila MA (2004) Anthocyanins and human health: an in vitro investigative approach. J Biomed Biotechnol 5:306–313CrossRefGoogle Scholar
  27. Masoudi-Nejad A, Nasuda S, McIntosh RA, Endo TR (2002) Transfer of rye chromosome segments to wheat by a gametocidal system. Chromosome Res 10:349–357PubMedCrossRefGoogle Scholar
  28. Matsumoto H, Nakamura Y, Tachibanaki S, Kawamura S, Hirayama M (2003) Stimulatory effect of cyanidin 3-glycosides on the regeneration of rhodopsin. J Agric Food Chem 51:3560–3563PubMedCrossRefGoogle Scholar
  29. Mazza G (2007) Anthocyanins and heart health. Annali dell’Istituto Superiore di Sanita 43:369–374PubMedGoogle Scholar
  30. Mazza G, Miniati E (1993) Anthocyanins in fruits, vegetables and grains. CRC Press Incorporation, Boca RatonGoogle Scholar
  31. Morrison LA, Metzger RJ, Lukaszewski AJ (2004) Origin of the Blue-Aleurone Gene in Sebesta blue wheat genetic stocks and a protocol for its use in apomixes screening. Crop Sci 44:2063–2067CrossRefGoogle Scholar
  32. Pisha E, Pezzuto JM (1994) Fruits and vegetables containing compounds that demonstrate pharmacological activity in humans. Econ Med Plant Res 6:189–233Google Scholar
  33. Qualset CO, Sollman KM, Jan C-C, Dvorak J, McGuire PE, Vogt HE (2005) Registration of UC66049 Triticum aestivum blue aleurone genetic stock. Crop Sci 45:432CrossRefGoogle Scholar
  34. Rechner AR, Kroner C (2005) Anthocyanins and colonic metabolites of dietary polyphenols inhibit platelet function. Thromb Res 116:327–334PubMedCrossRefGoogle Scholar
  35. Sears ER (1981) Transfer of alien genetic material to wheat. In: Evans LT, Peacock WJ (eds) Wheat science—today and tomorrow. Cambridge University Press, New York, pp 75–89Google Scholar
  36. Shi F, Endo TR (1999) Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilops cylindrica. Genes Genet Syst 74:49–54CrossRefGoogle Scholar
  37. Singh K, Ghai M, Garg M, Chhuneja P, Kaur P, Schnurbusch T, Keller B, Dhaliwal HS (2007) An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum × T. monococcum RIL population. Theor Appl Genet 115:301–312PubMedCrossRefGoogle Scholar
  38. Trojan V, Musilová M, Vyhnánek T, Klejdus B, Hanáček P, Havel L (2014) Chalcone synthase expression and pigment deposition in wheat with purple and blue colored caryopsis. J Cereal Sci 59:48–55CrossRefGoogle Scholar
  39. Wang J, Mazza G (2002) Effects of anthocyanins and other phenolic compounds on the production of tumor necrosis factor alpha in LPS/IFN-gamma-activated RAW 264.7 macrophages. J Agric Food Chem 50:4183–4189PubMedCrossRefGoogle Scholar
  40. Wang L-S, Stoner GD (2008) Anthocyanins and their role in cancer prevention. Cancer Lett 269:281–290PubMedCentralPubMedCrossRefGoogle Scholar
  41. Wang H, Cao GH, Prior RL (1997) Oxygen radical absorbing capacity of anthocyanins. J Agric Food Chem 45:304–309CrossRefGoogle Scholar
  42. Whelan EDP (1989) Transmission of an alien telocentric addition chromosome in common wheat that confers blue seed colour. Genome 32:30–34CrossRefGoogle Scholar
  43. William MDHM, Mujeeb-Kazi A (1993) Thinopyrum bessarabicum: biochemical and cytological markers for detection of genetic introgression in its hybrid derivatives with Triticum aestivum L. Theor Appl Genet 86:365–370PubMedGoogle Scholar
  44. Yang GH, Li B, Gao JW, Liu JZ, Zhao XQ, Zheng Q, Tong YP, Li ZS (2004) Cloning and expression of two chalcone synthase and a flavonoid 3′5′-hydroxylase 3′end cDNAs from developing seeds of blue-grained wheat involved in anthocyanin biosynthetic pathway. Acta Bot Sin 46:588–594Google Scholar
  45. Yoshinaga K (1986) Liquor with pigments of red rice. J Brew Soc Jpn 81:337–342CrossRefGoogle Scholar
  46. Youdim KA, Martin A, Joseph JA (2000) Incorporation of the elderberry anthocyanins by endothelial cells increases protection against oxidative stress. Free Rad Biol Med 29:51–60PubMedCrossRefGoogle Scholar
  47. Zeller FJ, Cermeno MC, Miller TE (1991) Cytological analysis on the distribution and origin of the alien chromosome pair conferring blue aleurone color in several European common wheat (Triticum aestivum L.) strains. Theor Appl Genet 81:551–558PubMedCrossRefGoogle Scholar
  48. Zeven AC (1991) Wheats with purple and blue grains: a review. Euphytica 56:243–258CrossRefGoogle Scholar
  49. Zhang XY, Dong YS, Wang RRC (1996) Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum × Thinopyrum ponticum by in situ hybridization, isozyme analysis and RAPD. Genome 39:1062–1071PubMedCrossRefGoogle Scholar
  50. Zheng Q, Li B, Zhang XY, Mu SM, Zhou HP, Li ZS (2006a) Physical mapping of the blue-grained gene from Thinopyrum ponticum by GISH and FISH in a set of translocation lines with different seed colors in wheat. Genome 49:1109–1114PubMedCrossRefGoogle Scholar
  51. Zheng Q, Li B, Zhang XY, Mu SM, Zhou HP, Li ZS (2006b) Molecular cytogenetic characterization of wheat-Thinopyrum ponticum translocations bearing blue-grained gene(s) induced by r-ray irradiation. Euphytica 152:51–60CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Veronika Burešová
    • 1
  • David Kopecký
    • 1
  • Jan Bartoš
    • 1
  • Petr Martinek
    • 2
  • Nobuyoshi Watanabe
    • 3
  • Tomáš Vyhnánek
    • 4
  • Jaroslav Doležel
    • 1
  1. 1.Institute of Experimental BotanyCentre of the Region Haná for Biotechnological and Agricultural ResearchOlomoucCzech Republic
  2. 2.Agrotest Fyto, Ltd.KroměřížCzech Republic
  3. 3.College of AgricultureIbaraki UniversityInashikiJapan
  4. 4.Mendel University in BrnoBrnoCzech Republic

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