Theoretical and Applied Genetics

, Volume 127, Issue 10, pp 2267–2277 | Cite as

Molecular mapping of genes Yr64 and Yr65 for stripe rust resistance in hexaploid derivatives of durum wheat accessions PI 331260 and PI 480016

  • P. Cheng
  • L. S. Xu
  • M. N. Wang
  • D. R. See
  • X. M. Chen
Original Paper

Abstract

Key message

This manuscript reports two new genes (Yr64andYr65) for effective resistance to stripe rust and usefulness of their flanking SSR markers for marker-assisted selection.

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide and resistance is the best control strategy. Durum wheat accessions PI 331260 and PI 480016 were resistant to all tested Pst races. To transfer the resistance genes to common wheat and map them to wheat chromosomes, both accessions were crossed with the stripe rust-susceptible spring wheat ‘Avocet S’. Resistant F3 plants with 42 chromosomes were selected cytologically and by rust phenotype. A single dominant gene for resistance was identified in segregating F4 lines from each cross. F6 populations for each cross were developed from single F5 plants and used for genetic mapping. Different genes from PI 331260 and PI 480016 were mapped to different loci in chromosome 1BS using simple sequence repeat markers. The gene from PI 331260 was flanked by Xgwm413 and Xgdm33 in bin 1BS9-0.84-1.06 at genetic distances of 3.5 and 2.0 cM; and the gene from PI 480016 was flanked by Xgwm18 and Xgwm11 in chromosome bin C-1BS10-0.50 at 1.2 and 2.1 cM, respectively. Chromosomal locations and race and allelism tests indicated that the two genes are different from previously reported stripe rust resistance genes, and therefore are named as Yr64 from PI 331260 and Yr65 from PI 480016. These genes and their flanking markers, and selected common wheat lines with the genes should be valuable for diversifying resistance genes used in breeding wheat cultivars with stripe rust resistance.

Supplementary material

122_2014_2378_MOESM1_ESM.docx (30 kb)
Supplement Table 1 Alleles of SSR markers flanking the Yr64 locus in PI 331260, the Yr65 locus in PI 480016, Avocet S (AvS) and 140 US Pacific Northwest wheat cultivars and breeding lines. (DOCX 29 kb)

References

  1. Abdalla O, Dieseth JA, Singh RP (1992) Breeding durum wheat at CIMMYT. In: Rajaram S, Saari EE, Hettel GP (eds) Durum wheats: challenge and opportunities. CIMMYT Wheat Special Report No. 9. CIMMYT, Mexico, pp 1–13Google Scholar
  2. Bariana HS, Brown GN, Ahmed NU, Khatkar S, Conner RL, Wellings CR, Haley S, Sharp PJ, Laroche A (2002) Characterisation of Triticum vavilovii-derived stripe rust resistance using genetic, cytogenetic and molecular analyses and its marker-assisted selection. Theor Appl Genet 104:315–320Google Scholar
  3. Chague V, Fahima T, Dahan A, Sun GL, Korol AB, Ronin YI, Grama A, Roder MS, Nevo E (1999) Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis. Genome 42:1050–1056PubMedCrossRefGoogle Scholar
  4. Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp tritici] on wheat. Can J Plant Pathol 27:314–337CrossRefGoogle Scholar
  5. Chen XM (2007) Challenges and solutions for stripe rust control in the United States. Aust J Agric Res 58:648–655CrossRefGoogle Scholar
  6. Chen XM (2013) Review article: high-temperature adult-plant resistance, key for sustainable control of stripe rust. Am J Plant Sci 4:608–627CrossRefGoogle Scholar
  7. Chen XM, Line RF (1992a) Inheritance of stripe rust resistance in wheat cultivars used to differentiate races of Puccinia striiformis in North America. Phytopathology 82:633–637CrossRefGoogle Scholar
  8. Chen XM, Line RF (1992b) Identification of stripe rust resistance genes in wheat genotypes used to differentiate North American races of Puccinia striiformis. Phytopathology 82:1428–1434CrossRefGoogle Scholar
  9. Chen XM, Jones SS, Line RF (1995a) Chromosomal location of genes for stripe rust resistance in spring wheat cultivars Compair, Fielder, Lee, and Lemhi and interactions of aneuploid wheats with races of Puccinia striiformis. Phytopathology 85:375–381CrossRefGoogle Scholar
  10. Chen XM, Line RF, Jones SS (1995b) Chromosomal location of genes for resistance to Puccinia striiformis in winter wheat cultivars Heines VII, Clement, Moro, Tyee, Tres, and Daws. Phytopathology 85:1362–1367CrossRefGoogle Scholar
  11. Chen XM, Penman L, Wan AM, Cheng P (2010) Virulence races of Puccinia striiformis f. sp tritici in 2006 and 2007 and development of wheat stripe rust and distributions, dynamics, and evolutionary relationships of races from 2000 to 2007 in the United States. Can J Plant Pathol 32:315–333CrossRefGoogle Scholar
  12. Cheng P, Chen XM (2010) Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s. Theor Appl Genet 121:195–204PubMedCrossRefGoogle Scholar
  13. Chhuneja P, Kaur S, Garg T, Ghai M, Kaur S, Prashar M, Bains NS, Goel RK, Keller B, Dhaliwal HS, Singh K (2008) Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat. Theor Appl Genet 116:313–324PubMedCrossRefGoogle Scholar
  14. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  15. Kunert A, Naz AA, Dedeck O, Pillen K, Léon J (2007) AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides x T. tauschii) as a source of favourable alleles for milling and baking quality traits. Theor Appl Genet 115:683–695PubMedCrossRefGoogle Scholar
  16. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg I (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedCrossRefGoogle Scholar
  17. Li GQ, Li ZF, Yang WY, Zhang Y, He ZH, Xu SC, Singh RP, Qu YY, Xia XC (2006) Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26. Theor Appl Genet 112:1434–1440PubMedCrossRefGoogle Scholar
  18. Li Q, Chen XM, Wang MN, Jing JX (2011) Yr45, a new wheat gene for stripe rust resistance on the long arm of chromosome 3D. Theor Appl Genet 122:189–197PubMedCrossRefGoogle Scholar
  19. Lin F, Chen XM (2007) Genetics and molecular mapping of genes for race-specific all-stage resistance and non-race specific high-temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theor Appl Genet 114:1277–1287PubMedCrossRefGoogle Scholar
  20. Line RF (2002) Stripe rust of wheat and barley in North America: a retrospective historical review. Annu Rev Phytopathol 40:75–118PubMedCrossRefGoogle Scholar
  21. Line RF, Qayoum A (1992) Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America. 1968–87 USDA-ARS. Tech Bull 1788:44Google Scholar
  22. Liu RH, Meng JL (2003) MapDraw: a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data. Yi Chuan 25:317–321PubMedGoogle Scholar
  23. Lu Y, Wang MN, Chen XM, See D, Chao SM, Jing JX (2014) Mapping of Yr62 and a small effect QTL for high-temperature adult-plant resistance to stripe rust in spring wheat PI 192252. Theor Appl Genet 127:1449–1459. doi:10.1007/s00122-014-2312-0 PubMedCrossRefGoogle Scholar
  24. Ma H, Singh RP, Mujeeb-Kazi A (1995) Resistance to stripe rust in Triticum turgidum, T. tauschii and their synthetic hexaploids. Euphytica 82:117–124CrossRefGoogle Scholar
  25. Ma H, Singh RP, Abdalla O (1997a) Resistance to stripe rust in five durum wheat cultivars. Plant Dis 81:27–30CrossRefGoogle Scholar
  26. Ma H, Singh RP, Mujeeb KA (1997b) Resistance to stripe rust in durum wheats, A-genome diploids, and their amphiploids. Euphytica 94:279–286CrossRefGoogle Scholar
  27. Ma JX, Zhou RH, Dong YS, Wang LF, Wang XM, Jia JZ (2001) Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica 120:219–226CrossRefGoogle Scholar
  28. Macer RCF (1966) The formal and monosomic genetic analysis of stripe rust (Puccinia striiformis) resistance in wheat. In: Proceedings of 2nd International Wheat Genetic Symposium, Lund, Sweden, pp 127–142Google Scholar
  29. Mamluk OF (1992) Durum wheat diseases in West Asia and North Africa (WANA). In: Rajaram S, Saari EE, Hettel GP (eds) Durum Wheats: Challegen and Opportunities. CIMMYT Wheat Special Report No. 9. CIMMYT, Mexico, pp 89–106Google Scholar
  30. McIntosh RA, Lagudah ES (2000) Cytogenetical studies in wheat. XVIII. Gene Yr24 for resistance to stripe rust. Plant Breed 119:81–83CrossRefGoogle Scholar
  31. McIntosh RA, Silk J, The TT (1996) Cytogenetical studies in wheat XVII. Monosomic analysis and linkage relationships of gene Yr15 for resistance to stripe rust. Euphytica 89:395–399Google Scholar
  32. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat. In: 12th International Wheat Genetic Symposium, 8–13 Sep 2013, Yokohama, Japan. Online: http://www.shigen.nig.ac.jp/wheat/komugi/genes/download.jsp. Accessed 8 Jan 2014
  33. Metzger RJ, Silbaugh BA (1970) Inheritance of resistance to stripe rust and its association with brown glume colour in Triticum aestivum L. PI 178383. Crop Sci 10:567–568CrossRefGoogle Scholar
  34. Murphy LR, Santra D, Kidwell K, Yan GP, Chen XM, Campbell KG (2009) Linkage maps of wheat stripe rust resistance genes Yr5 and Yr15 for Use in marker-assisted selection. Crop Sci 49:1786–1790CrossRefGoogle Scholar
  35. Peng JH, Fahima T, Roder MS, Huang QY, Dahan A, Li YC, Grama A, Nevo E (2000) High-density molecular map of chromosome region harboring stripe-rust resistance genes YrH52 and Yr15 derived from wild emmer wheat, Triticum dicoccoides. Genetica 109:199–210PubMedCrossRefGoogle Scholar
  36. Raats D, Frenkel Z, Krugman T, Dodek I, Sela H, Šimková H, Magni F, Cattonaro F, Vautrin S, Bergès H, Wicker T, Keller B, Leroy P, Philippe R, Paux E, Doležel J, Feuillet C, Korol A, Fahima T (2013) The physical map of wheat chromosome 1BS provides insights into its gene space organization and evolution. Genome Biol 14:R138PubMedCrossRefPubMedCentralGoogle Scholar
  37. Randhawa HS, Mutti JS, Kidwell K, Morris CF, Chen XM, Gill KS (2009) Rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection. PLoS One 4:e5752PubMedCrossRefPubMedCentralGoogle Scholar
  38. Ren RS, Wang MN, Chen XM, Zhang ZJ (2012) Characterization and molecular mapping of Yr52 for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI 183527. Theor Appl Genet 125:847–857PubMedCrossRefGoogle Scholar
  39. Riede CR, Anderson JA (1996) Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci 36:905–909CrossRefGoogle Scholar
  40. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedPubMedCentralGoogle Scholar
  41. Rosewarne GM, Herrera-Foessel SA, Singh RP, Huerta-Espino J, Lan CX, He ZH (2013) Quantitative trait loci of stripe rust resistance in wheat. Theor Appl Genet 126:2427–2449PubMedCrossRefPubMedCentralGoogle Scholar
  42. Sharma-Poudyal D, Chen XM (2011) Models for predicting potential yield loss of wheat caused by stripe rust in the U.S. Pacific Northwest. Phytopathology 101:544–554PubMedCrossRefGoogle Scholar
  43. Sharma-Poudyal D, Chen XM, Wan AM, Zhan GM, Kang ZS, Cao SQ, Jin SL, Morgounov A, Akin B, Mert Z, Shah SJA, Bux H, Ashraf M, Sharma RC, Madariaga R, Puri KD, Wellings C, Xi KQ, Wanyera R, Manninger K, Ganzález MI, Koyda M, Sanin S, Patzek LJ (2013) Virulence characterization of international collections of the wheat stripe rust pathogen, Puccinia striiformis f. sp. tritici. Plant Dis 97:379–386CrossRefGoogle Scholar
  44. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  45. Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25PubMedCrossRefGoogle Scholar
  46. Stubbs RW (1985) Stripe rust. In: Roelfs AP, Bushnell WR (eds) The Cereal Rusts, vol 2., Diseases, distribution, epidemiology and controlAcademic Press, Orlando, pp 61–101Google Scholar
  47. Uauy C, Brevis JC, Chen XM, Khan I, Jackson L, Chicaiza O, Distelfeld A, Fahima T, Dubcovsky J (2005) High-temperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 112:97–105PubMedCrossRefGoogle Scholar
  48. Wan AM, Chen XM (2012) Virulence, frequency, and distribution of races of Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei identified in the United States in 2008 and 2009. Plant Dis 96:67–74CrossRefGoogle Scholar
  49. Wan AM, Chen XM (2014) Virulence characterization of Puccinia striiformis f. sp. tritici using a new set of Yr single-gene line differentials in the United States in 2010. Plant Dis. 000:000-000. (In press)Google Scholar
  50. Wang LF, Ma JX, Zhou RH, Wang XM, Jia JZ (2002) Molecular tagging of the yellow rust resistance gene Yr10 in common wheat, P.I.178383 (Triticum aestivum L.). Euphytica 124:71–73CrossRefGoogle Scholar
  51. Wang CM, Zhang YP, Han DJ, Kang ZS, Chen PD (2008) SSR and STS markers for wheat stripe rust resistance gene Yr26. Euphytica 159:359–366CrossRefGoogle Scholar
  52. Wang MN, Chen XM, Xu LS, Cheng P, Bockelman H (2012) Registration of 70 common spring wheat germplasm lines resistant to stripe rust. J Plant Regist 6:104–110CrossRefGoogle Scholar
  53. Wellings CR (2011) Global status of stripe rust: a review of historical and current threats. Euphytica 179:129–141CrossRefGoogle Scholar
  54. Xu LS, Wang MN, Cheng P, Kang ZS, Hulbert SH, Chen XM (2013) Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat. Theor Appl Genet 126:523–533PubMedCrossRefGoogle Scholar
  55. Yan GP, Chen XM, Line RF, Wellings CR (2003) Resistance gene-analog polymorphism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust. Theor Appl Genet 106:636–643PubMedGoogle Scholar
  56. Yildirim A, Jones SS, Murray TD, Line RF (2000) Evaluation of Daspyrum villosum populations for resistance to cereal eyespot and stripe rust pathogens. Plant Dis 84:40–44CrossRefGoogle Scholar
  57. Zakari A, McIntosh RA, Hovmøller MS, Wellings CR, Shariflou MR, Hayden M, Bariana HS (2003) Recombination of Yr15 and Yr24 in chromosome 1BS. In: Pogna NE, Romano N, Pogna EA, Galterio G (eds.) Proceedings of 10th Internationla Wheat Genetic Symposium Instituto Sperimentale per la Cerealcoltura, Rome 1, pp 417–420Google Scholar
  58. Zhang X, Han D, Zeng Q, Duan Y, Yuan F, Shi J, Wang Q, Wu J, Huang L, Kang Z (2013) Fine mapping of wheat stripe rust resistance gene Yr26 based on collinearity of wheat with Brachypodium distachyon and rice. PLoS One 8:e57885PubMedCrossRefPubMedCentralGoogle Scholar
  59. Zhou XL, Wang MN, Chen XM, Lu Y, Kang ZS, Jing JX (2014) Identification of Yr59 conferring high-temperature adult-plant resistance to stripe rust in wheat germplasm PI 178759. Theor Appl Genet 127:935–945PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2014

Authors and Affiliations

  • P. Cheng
    • 1
  • L. S. Xu
    • 1
  • M. N. Wang
    • 1
  • D. R. See
    • 1
    • 2
  • X. M. Chen
    • 1
    • 2
  1. 1.Department of Plant PathologyWashington State UniversityPullmanUSA
  2. 2.Wheat Genetics, Quality, Physiology and Disease Research Unit, Agricultural Research ServiceUS Department of AgriculturePullmanUSA

Personalised recommendations