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Use of single nucleotide polymorphisms and haplotypes to identify genomic regions associated with protein content and water-soluble protein content in soybean

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Abstract

Key message

Four major SPC-specific loci were identified, and these accounted for 8.5–15.1 % of the phenotypic variation, thus explaining why certain soybean varieties have a high PC but a low SPC.

Abstract

Water-soluble protein content (SPC) is a critical factor in both food quality and the production of isolated soybean proteins. However, few data are available regarding the genetic control and the mechanisms contributing to elevated SPC. In this study, a soybean collection of 192 accessions from a wide geographic range was used to identify genomic regions associated with soybean protein content (PC) and SPC using an association mapping approach employing 1,536 SNP makers and 232 haplotypes. The diverse panel revealed a large genetic variation in PC and SPC. Association mapping was performed using three methods to minimize false-positive associations. This resulted in 4/8 SNPs and 3/6 haplotypes that were significantly associated with soybean PC/SPC in two or more environments based on the mixed model. An SNP that was highly significantly associated with PC, BARC-021267-04016, was localized 0.28 cM away from a published glycinin gene, G7, and was detected across all four environments. Four major SPC-specific loci, BARC-029149-06088, BARC-018023-02499, BARC-041663-08059 and haplotype 15 (hp15), were stably identified on chromosomes five and eight and explained 8.5–15.1 % of the phenotypic variation. Moreover, a glutelin type-B 2-like gene was identified on chromosome eight and may be related to soybean protein solubility. These markers, which are located in previously reported QTL, reconfirmed previous findings and may be important targets for the identification of protein-related genes. These novel SNPs and haplotypes are important for further understanding the genetic basis of PC and SPC. In addition, by comparing the correlation and genetic loci between PC and SPC, we provide new insights into why certain soybean varieties have a high protein content but a low SPC.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program) (2010CB125906), the National Natural Science Foundation of China (31171573, 31201230, 31301336, 31301341) and the Jiangsu Provincial Programs (BE2012328, BK2012768).

Conflict of interest

We declare that we have no conflicts of interest.

Author information

Correspondence to Dan Zhang.

Additional information

D. Zhang and G. Kan contributed equally to this work.

Communicated by Istvan Rajcan.

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Zhang, D., Kan, G., Hu, Z. et al. Use of single nucleotide polymorphisms and haplotypes to identify genomic regions associated with protein content and water-soluble protein content in soybean. Theor Appl Genet 127, 1905–1915 (2014). https://doi.org/10.1007/s00122-014-2348-1

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Keywords

  • Single Nucleotide Polymorphism
  • Association Mapping
  • Single Nucleotide Polymorphism Marker
  • Soybean Protein
  • Soybean Variety