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Theoretical and Applied Genetics

, Volume 127, Issue 9, pp 1873–1883 | Cite as

QTL mapping of adult-plant resistance to leaf rust in a RIL population derived from a cross of wheat cultivars Shanghai 3/Catbird and Naxos

  • Yue Zhou
  • Yan Ren
  • Morten Lillemo
  • Zhanjun Yao
  • Peipei Zhang
  • Xianchun Xia
  • Zhonghu He
  • Zaifeng Li
  • Daqun Liu
Original Paper

Abstract

Key message

Six QTL for adult plant resistance to leaf rust, including two QTL effective against additional diseases, were identified in a RIL population derived from a cross between Shanghai 3/Catbird and Naxos.

Abstract

Leaf rust is an important wheat disease and utilization of adult-plant resistance (APR) may be the best approach to achieve long-term protection from the disease. The CIMMYT spring wheat line Shanghai 3/Catbird (SHA3/CBRD) showed a high level of APR to Chinese Puccinia triticina pathotypes in the field. To identify APR genes in this line, a mapping population of 164 recombinant inbred lines (RILs) was developed from a cross of this line and Naxos, a moderately susceptible German cultivar. The RILs were evaluated for final disease severity (FDS) at Baoding, Hebei province, and Zhoukou, Henan province, in the 2010–2011 and 2011–2012 cropping seasons. QTL analysis detected one major QTL derived from SHA3/CBRD on chromosome 2BS explaining from 15 to 37 % of the phenotypic variance across environments. In addition one minor resistance QTL on chromosome 1AL from SHA3/CBRD and four minor QTL from Naxos on chromosomes 2DL, 5B, 7BS, and 7DS were also detected. SHA3/CBRD also possessed seedling resistance gene Lr26, and Naxos contained Lr1 based on gene postulation following tests with an array of P. triticina pathotypes and molecular marker assays. These seedling resistance and APR genes and their closely linked molecular markers are potentially useful for improving leaf rust resistance in wheat breeding programs.

Keywords

Powdery Mildew Simple Sequence Repeat Marker Leaf Rust Stripe Rust Leaf Rust Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are grateful to the critical review of this manuscript by Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, Australia. The project was supported by National Natural Science Foundation of China (31361140367 and 31300562), the National Key Basic Research Program of China (2013CB127700), International Science & Technology Cooperation Program of China, the China Agriculture Research System (CARS-3-1-3), the Key Project of Hebei Applied Foundation Research Plan (11960145D), and the scientific research team of Baoding University (KYTD2013001).

Conflict of interest

The authors declare that there are no conflicts of interest in the reported research.

Ethical standards

The authors note that this research is performed and reported in accordance with ethical standards of the scientifc conduct.

References

  1. Allard RW (1960) Principles of plant breeding. Wiley, New YorkGoogle Scholar
  2. Bansal UK, Hayden MJ, Venkata BP, Khanna R, Saini RG, Bariana HS (2008) Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in common wheat. Theor Appl Genet 117:307–312PubMedCrossRefGoogle Scholar
  3. Bjarko ME, Line RF (1988) Heritability and number of genes controlling leaf rust resistance in four cultivars of wheat. Phytopathology 78:457–461CrossRefGoogle Scholar
  4. Bossolini E, Krattinger SG, Keller B (2006) Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theor Appl Genet 113:1049–1062PubMedCrossRefGoogle Scholar
  5. Caldwell RM (1968) Breeding for general and/or specific plant disease resistance. In: Finlay KW, Shepherd KW (eds) Proc 3rd Int Wheat Genet Symp. Australian Academy of Sciences, Canberra, pp 263–272Google Scholar
  6. Chai JF, Zhou RH, Jia JZ, Liu X (2006) Development and application of a new codominant PCR marker for detecting 1BL.1RS wheat–rye chromosome translocations. Plant Breed 125:302–304CrossRefGoogle Scholar
  7. Dubin HJ, Johnson R, Stubbs RW (1989) Postulated genes to stripe rust in selected CIMMYT and related wheats. Plant Dis 73:472–475CrossRefGoogle Scholar
  8. Dyck PL (1977) Genetics of leaf rust reaction in three introductions of common wheat. Can J Genet Cytol 19:711–716Google Scholar
  9. Dyck PL (1987) The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29:467–469CrossRefGoogle Scholar
  10. Dyck PL, Samborski DJ (1982) The inheritance of resistance to Puccinia recondita in a group of common wheat cultivars. Can J Genet Cytol 24:273–283Google Scholar
  11. Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M, Gautier M-F, Joudrier P, Schlatter AR, Dubcovsky J, De la Pena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422PubMedCrossRefGoogle Scholar
  12. Herrera-Foessel SA, Lagudah ES, Huerta-Espino J, Hayden MJ, Bariana HS, Singh D, Singh RP (2011) New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor Appl Genet 122:239–249PubMedCrossRefGoogle Scholar
  13. Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L, Calvo-Salazar V, Lan C, Lagudah ES (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124:1475–1486PubMedCrossRefGoogle Scholar
  14. Herrera-Foessel SA, Singh RP, Lillemo M, Huerta-Espino J, Bhavani S, Singh S, Lan C, Calvo-Salazar V, Lagudah ES (2014) Lr67/Yr46 confers adult plant resistance to stem rust and powdery mildew in wheat. Theor Appl Genet. doi: 10.1007/s00122-013-2256-9 PubMedGoogle Scholar
  15. Knott (1989) The wheat-rust breeding for resistance. Monographs on Theoretical and Applied Genetics, 12th edn. Springer Berlin, GermanyCrossRefGoogle Scholar
  16. Kosambi DD (1944) The estimation of map distance from recombination values. Annu Eugen 12:172–175CrossRefGoogle Scholar
  17. Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363PubMedCrossRefGoogle Scholar
  18. Krattinger SG, Lagudah ES, Wicker T, Risk JM, Ashton AR, Selter LL, Matsumoto T, Keller B (2011) Lr34 multi-pathogen resistance ABC transporter: molecular analysis of homoeologous and orthologous genes in hexaploid wheat and other grass species. Plant J 65:392–403PubMedCrossRefGoogle Scholar
  19. Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898PubMedCrossRefGoogle Scholar
  20. Li HH, Ye GY, Wang JK (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374PubMedCentralPubMedCrossRefGoogle Scholar
  21. Li ZF, Lan CX, He ZH, Singh RP, Rosewarne GM, Chen XM, Xia XC (2014) Overview and application of QTL for adult plant resistance to leaf rust and powdery mildew in wheat. Crop Sci CROP-2014-02-0162Google Scholar
  22. Lillemo M, Asalf B, Singh RP, Huerta-Espino J, Chen XM, He ZH, Bjørnstad Å (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166PubMedCrossRefGoogle Scholar
  23. Long DL, Kolmer JA (1989) A North American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology 79:525–529CrossRefGoogle Scholar
  24. Lu QX, Bjørnstad Å, Ren Y, Asad MA, Xia XC, Chen XM, Ji F, Shi JR, Lillemo M (2012) Partial resistance to powdery mildew in German spring wheat ‘Naxos’ is based on multiple genes with stable effects in diverse environments. Theor Appl Genet 125:297–309PubMedCrossRefGoogle Scholar
  25. Manly KF, Cudmore RH Jr, Meer JM (2001) Map manager QTX, cross-platform software for genetic mapping. Genome 12:930–932Google Scholar
  26. McCartney CA, Somers DJ, McCallum BD, Thomas J, Humphreys DG, Menzies JG, Brown PD (2005) Microsatellite tagging of the leaf rust resistance gene Lr16 on wheat chromosome 2BSc. Mol Breed 15:329–337CrossRefGoogle Scholar
  27. McDonald BA, Linde C (2002) Pathogen population genetics, evolutionary potential, and durable resistance. Annu Rev Phytopathol 40:349–379PubMedCrossRefGoogle Scholar
  28. McIntosh RA (1992) Close genetic linkage of genes conferring adult plant resistance to leaf rust and stripe rust in wheat. Plant Pathol 41:523–527CrossRefGoogle Scholar
  29. McIntosh RA, Dyck PL (1975) Cytogenetical studies in wheat VII. Gene Lr23 for reaction to Puccinia recondita in Gabo and related cultivars. Aust J Biol Sci 28:201–211Google Scholar
  30. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat: 2013. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/2013/GeneCatalogueIntroduction.pdf
  31. Messmer MM, Seyfarth S, Keller M, Schachermayr G, Winzeler M, Zanetti S, Feuillet C, Keller B (2000) Genetic analysis of durable leaf rust resistance in winter wheat. Theor Appl Genet 100:419–431CrossRefGoogle Scholar
  32. Nelson JC, Singh RP, Autrique JE, Sorrells ME (1997) Mapping genes conferring and suppressing leaf rust resistance in wheat. Crop Sci 37:1928–1935CrossRefGoogle Scholar
  33. Parida SK, Anand Raj Kumar K, Dalal V, Singh NK, Mohapatra T (2006) Unigene derived microsatellite markers for the cereal genomes. Theor Appl Genet 112:808–817PubMedCrossRefGoogle Scholar
  34. Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697PubMedCrossRefGoogle Scholar
  35. Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Can J Res 26:496–500CrossRefGoogle Scholar
  36. Qiu JW, Schürch AC, Yahiaoui N, Dong LL, Fan HJ, Zhang ZJ, Keller B, Ling HQ (2007) Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat. Theor Appl Genet 115:159–168PubMedCrossRefGoogle Scholar
  37. Ren Y, He ZH, Li J, Lillemo M, Wu L, Bai B, Lu QG, Zhu HZ, Zhou G, Du JY, Lu QL, Xia XC (2012a) QTL mapping of adult-plant resistance to stripe rust in a population derived from common wheat cultivars Naxos and Shanghai 3/Catbird. Theor Appl Genet 125:1211–1221PubMedCrossRefGoogle Scholar
  38. Ren Y, Li ZF, He ZH, Wu L, Bai B, Lan CX, Wang CF, Zhou G, Zhu HZ, Xia XC (2012b) QTL mapping of adult-plant resistances to stripe rust and leaf rust in Chinese wheat cultivar Bainong 64. Theor Appl Genet 125:1253–1262PubMedCrossRefGoogle Scholar
  39. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedCentralPubMedGoogle Scholar
  40. Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, MexicoGoogle Scholar
  41. Schnurbusch T, Paillard S, Schori A, Messmer M, Schachermayr G, Winzeler M, Keller B (2004) Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat reveals a major resistance QTL in the Lr34 chromosome region. Theor Appl Genet 108:477–484PubMedCrossRefGoogle Scholar
  42. Seyfarth R, Feuillet C, Schachermayr G, Winzeler M, Keller B (1999) Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat. Theor Appl Genet 99:554–560PubMedCrossRefGoogle Scholar
  43. Seyfarth R, Feuillet C, Schachermayr G, Messmer M, Winzeler M, Keller B (2000) Molecular mapping of the adult-plant rust resistance gene Lr13 in wheat (Triticum aestivum L.). J Genet Breed 54:193–198Google Scholar
  44. Singh RP (1992) Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology 82:835–838CrossRefGoogle Scholar
  45. Singh RP, Mujeeb-Kazi A, Huerta-Espino J (1998) Lr46: a gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathology 88:890–894PubMedCrossRefGoogle Scholar
  46. Singh RP, Huerta-Espino J, Rajaram S (2000a) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Entomol Hung 35:133–139Google Scholar
  47. Singh RP, Nelson JC, Sorrells ME (2000b) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155CrossRefGoogle Scholar
  48. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  49. Song QJ, Fickus EW, Cregan PB (2002) Characterization of trinucleotide SSR motifs in wheat. Theor Appl Genet 104:286–293PubMedCrossRefGoogle Scholar
  50. Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25PubMedCrossRefGoogle Scholar
  51. Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111:731–735PubMedCrossRefGoogle Scholar
  52. William M, Singh RP, Huerta-Espino J, Ortiz Islas S, Hoisington D (2003) Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93:153–159PubMedCrossRefGoogle Scholar
  53. Xue SL, Zhang ZZ, Lin F, Kong ZX, Cao Y, Li CJ, Yi HY, Mei MF, Zhu HL, Wu JZ, Xu HB, Zhao DM, Tian DG, Zhang CQ, Ma ZQ (2008) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117:181–189PubMedCrossRefGoogle Scholar
  54. Yu JK, Dake TM, Singh S, Benscher D, Li WL, Gill B, Sorrells ME (2004) Development and mapping of EST-derived simple sequence repeat markers for hexaploid wheat. Genome 47:805–818PubMedCrossRefGoogle Scholar
  55. Zhang LJ, Li ZF, Lillemo M, Xia XC, Liu DQ, Yang WX, Luo JC, Wang HY (2009) QTL mapping for adult-plant resistance to leaf rust in CIMMYT wheat cultivar Saar. Sci Agric Sin 42:388–397Google Scholar
  56. Zhao XL, Zheng TC, Xia XC, He ZH, Liu DQ, Yang WX, Yin GH, Li ZF (2008) Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 117:1069–1075PubMedCrossRefGoogle Scholar
  57. Zhou HX, Xia XC, He ZH, Li X, Wang CF, Li ZF, Liu DQ (2013) Molecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671. Theor Appl Genet 126:2141–2147PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Yue Zhou
    • 1
    • 2
  • Yan Ren
    • 3
  • Morten Lillemo
    • 4
  • Zhanjun Yao
    • 1
  • Peipei Zhang
    • 1
  • Xianchun Xia
    • 5
  • Zhonghu He
    • 5
    • 6
  • Zaifeng Li
    • 1
  • Daqun Liu
    • 1
  1. 1.Department of Plant Pathology, College of Plant ProtectionHebei Agricultural University, Biological Control Center for Plant Diseases and Plant Pests of HebeiBaodingChina
  2. 2.Baoding UniversityBaodingChina
  3. 3.College of AgronomyHenan Agricultural UniversityZhengzhouChina
  4. 4.Department of Plant SciencesNorwegian University of Life SciencesÅsNorway
  5. 5.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS)BeijingChina
  6. 6.International Maize and Wheat Improvement Center (CIMMYT) China OfficeBeijingChina

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