Theoretical and Applied Genetics

, Volume 127, Issue 6, pp 1449–1459 | Cite as

Mapping of Yr62 and a small-effect QTL for high-temperature adult-plant resistance to stripe rust in spring wheat PI 192252

  • Yan Lu
  • Meinan Wang
  • Xianming Chen
  • Deven See
  • Shiaoman Chao
  • Jinxue Jing
Original Paper

Abstract

Key message

This manuscript reports a new gene (Yr62) and a small-effect QTL for potentially durable resistance to stripe rust and usefulness ofYr62markers for marker-assisted selection.

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease of wheat worldwide. Spring wheat germplasm PI 192252 showed a high level of high-temperature adult-plant (HTAP) resistance to stripe rust in germplasm evaluation over 8 years in the State of Washington. To elucidate the genetic basis of resistance, PI 192252 was crossed with ‘Avocet susceptible’. A mapping population of 150 F5 recombinant inbred lines was developed using single-seed descent. Stripe rust tests were conducted with selected Pst races in a greenhouse and in field conditions under natural infections. The relative area under the disease progress curve (rAUDPC) data showed continuous distributions, indicating that HTAP resistance of PI 192252 was controlled by quantitative trait loci (QTL). Two QTL were identified in PI 192252, explaining 74.2 % of the total phenotypic variation for rAUDPC. These two QTL were mapped to chromosomes 4BL (QYrPI192252.wgp-4BL) and 5BS (QYrPI192252.wgp-5BS) with SSR and SNP markers and explained 40–60 and 22–27 %, respectively, of the phenotypic variation across the four environments. Because the major-effect QTL on 4BL is different from previously named Yr genes and inherited as a single gene, it is named Yr62. The SSR marker alleles Xgwm192222 and Xgwm251133 flanking Yr62 were different from the alleles in various wheat varieties, suggesting that these markers could be useful in marker-assisted selection for incorporating Yr62 into commercial cultivars.

Supplementary material

122_2014_2312_MOESM1_ESM.docx (24 kb)
Supplementary Table 1 (DOCX 24 kb)

References

  1. Agenbag GM, Pretorius ZA, Boyd LA, Bender CM, Prins R (2012) Identification of adult plant resistance to stripe rust in the wheat cultivar Cappelle–Desprez. Theor Appl Genet 125:109–120PubMedCrossRefGoogle Scholar
  2. Bansal U, Forrest K, Hayden M, Miah H, Singh D, Bariana H (2011) Characterisation of a new stripe rust resistance gene Yr47 and its genetic association with the leaf rust resistance gene Lr52. Theor Appl Genet 122:1461–1466PubMedCrossRefGoogle Scholar
  3. Cavanagh CR, Chao S, Wang S, Huang BE, Stephen S, Kiani S, Forrest K, Saintenac C, Brown-Guedira GL, Akhunova A, See D, Bai G, Pumphrey M, Tomar L, Wong D, Kong S, Reynolds M, da Silva ML, Bockelman H, Talbert L, Anderson JA, Dreisigacker S, Baenziger S, Carter A, Korzun V, Morrell PL, Dubcovsky J, Morell MK, Sorrells ME, Hayden MJ, Akhunov E (2013) Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proc Natl Acad Sci USA 110:8057–8062PubMedCentralPubMedCrossRefGoogle Scholar
  4. Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337CrossRefGoogle Scholar
  5. Chen XM (2007) Challenges and solutions for stripe rust control in the United States. Aust J Agric Res 58:648–655CrossRefGoogle Scholar
  6. Chen XM (2013) Review article: high-temperature adult-plant resistance, key for sustainable control of stripe rust. Am J Plant Sci 4:608–627CrossRefGoogle Scholar
  7. Chen XM, Line RF (1992a) Identification of stripe rust resistance genes in wheat cultivars used to differentiate North American races of Puccinia striiformis. Phytopathology 82:1428–1434CrossRefGoogle Scholar
  8. Chen XM, Line RF (1992b) Inheritance of stripe rust resistance in wheat cultivars used to differentiate races of Puccinia striiformis in North America. Phytopathology 82:633–637CrossRefGoogle Scholar
  9. Chen XM, Line RF (1995a) Gene action in wheat cultivars for durable high-temperature adult-plant resistance and interactions with race-specific, seedling resistance to stripe rust caused by Puccinia striiformis. Phytopathology 85:567–572CrossRefGoogle Scholar
  10. Chen XM, Line RF (1995b) Gene number and inheritability of wheat cultivars with durable, high-temperature, adult-plant resistance and race-specific resistance to Puccinia striiformis. Phytopathology 85:573–578CrossRefGoogle Scholar
  11. Chen XM, Penman L, Wan AM, Cheng P (2010) Virulence races of Puccinia striiformis f. sp. tritici in 2006 and 2007 and development of wheat stripe rust and distributions, dynamics, and evolutionary relationships of races from 2000 to 2007 in the United States. Can J Plant Pathol 32:315–333CrossRefGoogle Scholar
  12. Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307CrossRefGoogle Scholar
  13. Hao YF, Chan ZB, Wang YY, Bland D, Buck J, Brown-Guedira G, Johnson J (2011) Characterization of a major QTL for adult plant resistance to stripe rust in US soft red winter wheat. Theor Appl Genet 123:1401–1411PubMedCrossRefGoogle Scholar
  14. He ZH, Lan CX, Chen XM, Zou YC, Zhuang QS, Xia XC (2011) Progress and perspective in research of adult-plant resistance to stripe rust and powdery mildew in wheat. Sci Agric Sin 44:2193–2215Google Scholar
  15. Jagger LJ, Newell C, Berry ST, MacCormack R, Boyd LA (2011) The genetic characterisation of stripe rust resistance in the German wheat cultivar Alcedo. Theor Appl Genet 122:723–733PubMedCrossRefGoogle Scholar
  16. Johnson R (1981) Durable resistance: definition, genetic control, and attainment in plant breeding. Phytopathology 71:567–568CrossRefGoogle Scholar
  17. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  18. Lin F, Chen XM (2007) Genetics and molecular mapping of genes for race-specific all-stage resistance and non-race-specific high temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theor Appl Genet 114:1277–1287PubMedCrossRefGoogle Scholar
  19. Line RF (2002) Stripe rust of wheat and barley in North America: a retrospective historical review. Annu Rev Phytopathol 40:75–118PubMedCrossRefGoogle Scholar
  20. Line RF, Chen XM (1995) Successes in breeding for and managing durable resistance to wheat rusts. Plant Dis 79:1254–1255Google Scholar
  21. Line RF, Qayoum A (1992) Virulence aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968-87. U.S. Department of Agriculture Technical Bulletin No. 1788, the National Technical Information Service, Springfield, p 44Google Scholar
  22. Liu J, Chang ZJ, Zhang XJ, Yang ZJ, Li X, Jia JQ, Zhan HX, Guo HJ, Wang JM (2013) Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL. Theor Appl Genet 126:265–274PubMedCrossRefGoogle Scholar
  23. Lu YM, Lan CX, Liang SS, Zhou XC, Liu D, Zhou G, Lu QL, Jing JX, Wang MN, Xia XC, He ZH (2009) QTL mapping for adult plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli. Theor Appl Genet 119:1349–1359PubMedCrossRefGoogle Scholar
  24. Melichar JPE, Berry S, Newell C, MacCormack R, Boyd LA (2008) QTL identification and microphenotype characterization of the developmentally regulated yellow rust resistance in the UK wheat cultivar Guardian. Theor Appl Genet 117:391–399PubMedCrossRefGoogle Scholar
  25. Milus EA, Line RF (1986) Number of genes controlling high temperature adult-plant resistance to stripe rust in wheat. Phytopathology 76:93–96CrossRefGoogle Scholar
  26. Qayoum A, Line RF (1985) High-temperature, adult-plant resistance to stripe rust of wheat. Phytopathology 75:1121–1125CrossRefGoogle Scholar
  27. Riede CR, Anderson JA (1996) Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci 36:905–909CrossRefGoogle Scholar
  28. Sears ER (1966) Nullisomic–tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis KR (eds) Chromosome manipulations and plant genetics. Oliver and Boyd, Edinburgh, pp 29–45CrossRefGoogle Scholar
  29. Sears ER, Sears LMS (1978) The telocentric chromosomes of common wheat. In: Ramanujan S (ed) Proceedings of the 5th International Wheat Genet Symposium Indian Society of Genetics and Plant Breeding. Indian Agri Res Inst, New Delhi, pp 389–407Google Scholar
  30. Sharma-Poudyal D, Chen XM, Wan AM, Zhan GM, Kang ZS, Cao SQ, Jin SL, Morgounov A, Akin B, Mert Z, Shah SJA, Bux H, Ashraf M, Sharma RC, Madariaga R, Puri KD, Wellings C, Xi KQ, Wanyera R, Manninger K, Ganzález MI, Koyda M, Sanin S, Patzek LJ (2013) Virulence characterization of international collections of the wheat stripe rust pathogen, Puccinia striiformis f. sp. tritici. Plant Dis 97:379–386CrossRefGoogle Scholar
  31. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  32. Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25PubMedCrossRefGoogle Scholar
  33. Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890PubMedCrossRefGoogle Scholar
  34. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  35. Wan AM, Chen XM (2012) Virulence, frequency, and distribution of races of Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei identified in the United States in 2008 and 2009. Plant Dis 96:67–74CrossRefGoogle Scholar
  36. Wang S, Basten JC, Zeng ZB (2010) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, RaleighGoogle Scholar
  37. Wang MN, Chen XM, Xu LS, Cheng P, Bockelman H (2012) Registration of 70 common spring wheat germplasm lines resistant to stripe rust. J Plant Regist 6:104–110CrossRefGoogle Scholar
  38. Wellings CR, Boyd LA, Chen XM (2012) Resistance to stripe rust in wheat: Pathogen biology driving resistance breeding. In: Sharma I (ed) Disease resistance in wheat. CAB International, Wallingford, pp 63–83CrossRefGoogle Scholar
  39. William HM, Singh RP, Huerta-Espino J, Palacios G, Suenaga K (2006) Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat. Genome 49:977–990PubMedCrossRefGoogle Scholar
  40. Yang J, Bai G, Shaner GE (2005) Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang. Theor Appl Genet 111:1571–1579PubMedCrossRefGoogle Scholar
  41. Zwart RS, Thompson JP, Milgate AW, Bansal UK, Williamson PM, Raman H, Bariana HS (2010) QTL mapping of multiple foliar disease and root-lesion nematode resistances in wheat. Mol Breed 26:107–124CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2014

Authors and Affiliations

  • Yan Lu
    • 1
    • 2
  • Meinan Wang
    • 2
  • Xianming Chen
    • 2
    • 3
  • Deven See
    • 2
    • 3
  • Shiaoman Chao
    • 4
  • Jinxue Jing
    • 1
  1. 1.State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant ProtectionNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Department of Plant PathologyWashington State UniversityPullmanUSA
  3. 3.US Department of AgricultureAgricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research UnitPullmanUSA
  4. 4.US Department of AgricultureAgricultural Research Service, Cereal Crops ResearchFargoUSA

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