Theoretical and Applied Genetics

, Volume 127, Issue 4, pp 935–945 | Cite as

Identification of Yr59 conferring high-temperature adult-plant resistance to stripe rust in wheat germplasm PI 178759

  • X. L. Zhou
  • M. N. Wang
  • X. M. Chen
  • Y. Lu
  • Z. S. Kang
  • J. X. Jing
Original Paper


Key message

This manuscript reports a new gene for non-race-specific resistance to stripe rust and molecular markers for incorporating it into wheat cultivars for control of the disease with durable resistance.


Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most destructive wheat diseases worldwide. The spring wheat germplasm ‘PI 178759’ originating from Iraq showed effective resistance to stripe rust in field evaluations over 8 years in Washington state, USA. To map the resistance gene(s), PI 178759 was crossed with ‘Avocet Susceptible’, and the parents and 176 F2:3 lines were phenotyped in the fields under natural infection and in a greenhouse with selected races of P. striiformis f. sp. tritici. PI 178759 was identified to have high-temperature adult-plant (HTAP) resistance. Resistance gene analog polymorphism and simple sequence repeat techniques were used to identify molecular markers linked to the resistance gene and a chromosome region was mapped using a quantitative trait locus approach. One major gene was mapped to the long arm of chromosome 7B. Flanked by Xwgp5175 and Xbarc32 in a 2.1 cM region, the gene explained 31.8 and 54.7 % of the phenotypic variation in rAUDPC and IT, respectively. Based on genetic distances among markers and allelism tests, the HTAP resistance gene in PI 178759 is different from the previously reported Yr39, Yr52, YrZH84, and YrC591, also located on chromosome 7BL, and is therefore designated as Yr59. The gene and its flanking markers should be useful for developing wheat cultivars with durable resistance.

Supplementary material

122_2014_2269_MOESM1_ESM.docx (33 kb)
Supplementary material 1 (DOCX 32 kb)
122_2014_2269_MOESM2_ESM.pptx (1.2 mb)
Supplementary material 2 (PPTX 1230 kb)


  1. Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337CrossRefGoogle Scholar
  2. Chen XM (2007) Challenges and solutions for stripe rust control in the United States. Aust J Agric Res 58:648–655CrossRefGoogle Scholar
  3. Chen XM (2013) Review article: high-temperature adult-plant resistance, key for sustainable control of stripe rust. Am J Plant Sci 4:608–627CrossRefGoogle Scholar
  4. Chen XM, Line RF (1992a) Inheritance of stripe rust resistance in wheat cultivars used to differentiate races of Puccinia striiformis in North America. Phytopathology 82:633–637CrossRefGoogle Scholar
  5. Chen XM, Line RF (1992b) Identification of stripe rust resistance genes in wheat genotypes used to differentiate North American races of Puccinia striiformis. Phytopathology 82:1428–1434CrossRefGoogle Scholar
  6. Chen XM, Line RF (1995a) Gene action in wheat cultivars for durable high-temperature adult-plant resistance and interactions with race-specific, seedling resistance to stripe rust caused by Puccinia striiformis. Phytopathology 85:567–572CrossRefGoogle Scholar
  7. Chen XM, Line RF (1995b) Gene number and heritability of wheat cultivars with durable, high-temperature, adult-plant resistance and race-specific resistance to Puccinia striiformis. Phytopathology 85:573–578CrossRefGoogle Scholar
  8. Chen XM, Line RF, Leung H (1998) Genome scanning for resistance gene analogs in rice, barley and wheat by high-resolution electrophoresis. Theor Appl Genet 97:345–355CrossRefGoogle Scholar
  9. Chen XM, Penman L, Wan AM, Cheng P (2010) Virulence races of Puccinia striiformis f. sp. tritici in 2006 and 2007 and development of wheat stripe rust and distributions, dynamics, and evolutionary relationships of races from 2000 to 2007 in the United States. Can J Plant Pathol 32:315–333CrossRefGoogle Scholar
  10. Chen XM, Coram T, Huang XL, Wang MN, Dolezal A (2013) Understanding molecular mechanisms of durable and non-durable resistance to stripe rust in wheat using a transcriptomics approach. Curr Genom 14:111–126CrossRefGoogle Scholar
  11. Cheng P, Chen XM (2010) Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s. Theor Appl Genet 121:195–204PubMedCrossRefGoogle Scholar
  12. Huang XQ, Röder MS (2004) Molecular mapping of powdery resistance genes in wheat: a review. Euphytica 137:203–223CrossRefGoogle Scholar
  13. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  14. Li ZF, Zheng TC, He ZH, Li GQ, Xu SC, Li XP, Yang GY, Singh RP, Xia XC (2006) Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 112:1098–1103PubMedCrossRefGoogle Scholar
  15. Li HH, Ye GY, Wang JK (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374PubMedCentralPubMedCrossRefGoogle Scholar
  16. Li HH, Ribaut JM, Li Z, Wang JK (2008) Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations. Theor Appl Genet 116:243–260PubMedCrossRefGoogle Scholar
  17. Li Y, Niu YC, Chen XM (2009) Mapping a stripe rust resistance gene YrC591 in wheat variety C591 with SSR and AFLP markers. Theor Appl Genet 118:339–346PubMedCrossRefGoogle Scholar
  18. Li Q, Chen XM, Wang MN, Jing JX (2011) Yr45, a new wheat gene for stripe rust resistance mapped on the long arm of chromosome 3D. Theor Appl Genet 122:189–197PubMedCrossRefGoogle Scholar
  19. Lin F, Chen XM (2007) Genetic and molecular mapping of genes for race-specific all-stage resistance and non-race-specific high temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theor Appl Genet 114:1277–1287PubMedCrossRefGoogle Scholar
  20. Lin F, Chen XM (2009) Quantitative trait loci for non-race-specific, high-temperature adult-plant resistance to stripe rust in wheat cultivar express. Theor Appl Genet 118:631–642PubMedCrossRefGoogle Scholar
  21. Line RF (2002) Stripe rust of wheat and barley in North America: a retrospective historical review. Annu Rev Phytopathol 40:75–118PubMedCrossRefGoogle Scholar
  22. Line RF, Qayoum A (1992) Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968–87. US Dep Agric Tech Bull 1788:44Google Scholar
  23. McIntosh R, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers D, Appels R, Devos K (2008) Catalogue of gene symbols for wheat. In: Proceedings of 11th international wheat genetic symposium, University of Sydney Press, Australia.
  24. Qayoum A, Line RF (1985) High-temperature, adult-plant resistance to stripe rust of wheat. Phytopathology 75:1121–1125CrossRefGoogle Scholar
  25. Ren RS, Wang MN, Chen XM, Zhang ZJ (2012) Characterization and molecular mapping of a new gene for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI 183527. Theor Appl Genet 125:847–857PubMedCrossRefGoogle Scholar
  26. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:207–333Google Scholar
  27. Sears ER (1966) Nullisomic-tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis KR (eds) Chromosome manipulations and plant genetics. Oliver and Boyd, Edinburgh, pp 29–45CrossRefGoogle Scholar
  28. Shi ZX, Chen XM, Line RF, Leung H, Wellings CR (2001) Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome 44:509–516PubMedCrossRefGoogle Scholar
  29. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  30. Sourdille P, Gandon B, Chiquet V, Nicot N, Somers D, Murigneux A, Bernard M (2005) Wheat genoplante SSR mapping data release: a new set of markers and comprehensive genetic and physical mapping data. Accessed 26 Nov 2012
  31. Stubbs RW (1985) Stripe rust. In: Roelfs AP, Bushnell WR (eds) The cereal rusts, vol II. Academic Press, NY, pp 61–101Google Scholar
  32. Wan AM, Chen XM (2012) Virulence, frequency, and distribution of races of Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei identified in the United States in 2008 and 2009. Plant Dis 96:67–74CrossRefGoogle Scholar
  33. Wang JK (2009) Inclusive composite interval mapping of quantitative trait genes. Acta Agron Sinica 35:239–245CrossRefGoogle Scholar
  34. Wang MN, Chen XM, Xu LS, Cheng P, Bockelman H (2012) Registration of 70 common spring wheat germplasm lines resistant to stripe rust. J Plant Regist 6:104–110CrossRefGoogle Scholar
  35. Wellings CR, McIntosh RA (1990) Puccinia striiformis f. sp. tritici in Australasia: pathogenic changes during the first 10 years. Plant Pathol 39:316–325CrossRefGoogle Scholar
  36. Yan GP, Chen XM, Line RF, Wellings CR (2003) Resistance gene-analog polymorphism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust. Theor Appl Genet 106:636–643PubMedGoogle Scholar
  37. Yang J, Bai G, Shaner GE (2005) Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang. Theor Appl Genet 111:1571–1579PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2014

Authors and Affiliations

  • X. L. Zhou
    • 1
    • 2
  • M. N. Wang
    • 2
  • X. M. Chen
    • 2
    • 3
  • Y. Lu
    • 1
    • 2
  • Z. S. Kang
    • 1
  • J. X. Jing
    • 1
  1. 1.State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant ProtectionNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Department of Plant PathologyWashington State, UniversityPullmanUSA
  3. 3.Wheat Genetics, Quality, Physiology and Disease Research UnitUS Department of Agriculture, Agricultural Research ServicePullmanUSA

Personalised recommendations